Early Gut Microbiota Profile in Healthy Neonates: Microbiome Analysis of the First-Pass Meconium Using Next-Generation Sequencing Technology
Abstract
:1. Introduction
2. Materials and Methods
2.1. Study Population and Sampling
2.2. DNA Extraction and Sequencing
2.3. Bioinformatic Analysis
3. Results
3.1. Study Cohort
3.2. Distinct Microbiota Community Structures in the Meconium Samples and Control Samples
3.3. A Similar Profile of the Microbiota Composition in the First-Pass Meconium
3.4. Relationship between Perinatal Characteristics and the First-Pass Meconium Microbiome
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Characteristics | N = 39 |
---|---|
Gender | |
Male | 19 |
Female | 20 |
Gestational age (weeks) | 38.1 (35.2–40.0) |
Birth weight (g) | 2880 (2020–3620) |
Mode of delivery | |
Vaginal | 19 |
C-section | 20 |
Primigravida | 9 (23.0%) |
Mother’s age (years) | 33.8 (23.9–45.9) |
Maternal intrapartum antibiotic treatment | 21 (53.8%) |
Prolonged rupture of membranes | 12 (30.7%) |
Feeding style | |
Exclusive breastfeeding or formula feeding | 0 |
Mixed (breast + formula feeding) | 39 (100%) |
Level | Organism | Abundance (%) | Level | Organism | Abundance (%) |
---|---|---|---|---|---|
Phylum | Proteobacteria | 85.45 | Family | Pseudomonadaceae | 77.11 |
Firmicutes | 9.74 | Enterococcaceae | 4.30 | ||
Bacteroidetes | 2.42 | Enterobacteriaceae | 3.88 | ||
Actinobacteria | 2.25 | Lactobacillaceae | 2.91 | ||
Fusobacteria | 0.03 | Sphingobacteriaceae | 1.43 | ||
Class | Gammaproteobacteria | 82.21 | Genus | Pseudomonas | 77.09 |
Bacilli | 9.44 | Enterococcus | 4.30 | ||
Actinobacteria | 2.25 | Escherichia/Shigella | 3.60 | ||
Alphaproteobacteria | 1.96 | Lactobacillus | 2.91 | ||
Sphingobacteriia | 1.43 | Sphingobacterium | 1.42 | ||
Order | Pseudomonadales | 78.06 | Species | Pseudomonas sp. (EU557337) | 17.35 |
Lactobacillales | 8.49 | Streptococcus salivarius | 0.83 | ||
Enterobacteriales | 3.88 | Parabacteroides distasonis | 0.69 | ||
Actinomycetales | 1.80 | Enterococcus faecalis | 0.65 | ||
Sphingobacteriales | 1.43 | Methylobacterium komagatae | 0.50 |
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Chang, Y.-S.; Li, C.-W.; Chen, L.; Wang, X.-A.; Lee, M.-S.; Chao, Y.-H. Early Gut Microbiota Profile in Healthy Neonates: Microbiome Analysis of the First-Pass Meconium Using Next-Generation Sequencing Technology. Children 2023, 10, 1260. https://doi.org/10.3390/children10071260
Chang Y-S, Li C-W, Chen L, Wang X-A, Lee M-S, Chao Y-H. Early Gut Microbiota Profile in Healthy Neonates: Microbiome Analysis of the First-Pass Meconium Using Next-Generation Sequencing Technology. Children. 2023; 10(7):1260. https://doi.org/10.3390/children10071260
Chicago/Turabian StyleChang, Yi-Sheng, Chang-Wei Li, Ling Chen, Xing-An Wang, Maw-Sheng Lee, and Yu-Hua Chao. 2023. "Early Gut Microbiota Profile in Healthy Neonates: Microbiome Analysis of the First-Pass Meconium Using Next-Generation Sequencing Technology" Children 10, no. 7: 1260. https://doi.org/10.3390/children10071260