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Article

The Azurin-Derived Peptide CT-p19LC Exhibits Membrane-Active Properties and Induces Cancer Cell Death

1
iBB-Institute for Bioengineering and Biosciences, Biological Sciences Research Group, Instituto Superior Técnico, University of Lisbon, Av. Rovisco Pais, 1, 1049-001 Lisbon, Portugal
2
Associate Laboratory i4HB—Institute for Health and Bioeconomy, Instituto Superior Técnico, University of Lisbon, Av. Rovisco Pais, 1, 1049-001 Lisbon, Portugal
3
Department of Bioengineering, Instituto Superior Técnico, University of Lisbon, Av. Rovisco Pais, 1, 1049-001 Lisbon, Portugal
*
Author to whom correspondence should be addressed.
Academic Editor: Leonardo Caputo
Biomedicines 2021, 9(9), 1194; https://doi.org/10.3390/biomedicines9091194
Received: 11 August 2021 / Revised: 30 August 2021 / Accepted: 6 September 2021 / Published: 10 September 2021
(This article belongs to the Special Issue Recent Advances in the Discovery of Novel Drugs on Natural Molecules)
Peptides have been thoroughly studied as new therapeutic strategies for cancer treatment. In this work, we explored in vitro the anticancer potential of three novel peptides derived from the C-terminal of azurin, an anticancer bacterial protein produced by Pseudomonas aeruginosa. CT-p26, CT-p19 and CT-p19LC peptides were previously obtained through an in silico peptide design optimization process, CT-p19LC being the most promising as it presented higher hydrophobicity and solubility, positive total charge and, most importantly, greater propensity for anticancer activity. Therefore, in this study, through proliferation and apoptosis assays, CT-p19LC was tested in four cancer cell lines—A549, MCF-7, HeLa and HT-29—and in two non-cancer cell lines—16HBE14o- and MCF10A. Its membrane-targeting activity was further evaluated with zeta potential measurements and membrane order was assessed with the Laurdan probe. The results obtained demonstrated that CT-p19LC decreases cell viability through induction of cell death and binds to the plasma membrane of cancer cells, but not to non-cancer cells, making them less rigid. Overall, this study reveals that CT-p19LC is an auspicious selective anticancer peptide able to react with cancer cell membranes and cause effective action. View Full-Text
Keywords: anticancer peptide; azurin; peptide-based drug development; cytotoxic effect; membrane-based anticancer therapy anticancer peptide; azurin; peptide-based drug development; cytotoxic effect; membrane-based anticancer therapy
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MDPI and ACS Style

Garizo, A.R.; Coelho, L.F.; Pinto, S.; Dias, T.P.; Fernandes, F.; Bernardes, N.; Fialho, A.M. The Azurin-Derived Peptide CT-p19LC Exhibits Membrane-Active Properties and Induces Cancer Cell Death. Biomedicines 2021, 9, 1194. https://doi.org/10.3390/biomedicines9091194

AMA Style

Garizo AR, Coelho LF, Pinto S, Dias TP, Fernandes F, Bernardes N, Fialho AM. The Azurin-Derived Peptide CT-p19LC Exhibits Membrane-Active Properties and Induces Cancer Cell Death. Biomedicines. 2021; 9(9):1194. https://doi.org/10.3390/biomedicines9091194

Chicago/Turabian Style

Garizo, Ana R., Lígia F. Coelho, Sandra Pinto, Tiago P. Dias, Fábio Fernandes, Nuno Bernardes, and Arsénio M. Fialho. 2021. "The Azurin-Derived Peptide CT-p19LC Exhibits Membrane-Active Properties and Induces Cancer Cell Death" Biomedicines 9, no. 9: 1194. https://doi.org/10.3390/biomedicines9091194

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