The FcγRIII Engagement Augments PMA-Stimulated Neutrophil Extracellular Traps (NETs) Formation by Granulocytes Partially via Cross-Talk between Syk-ERK-NF-κB and PKC-ROS Signaling Pathways
Abstract
:1. Introduction
2. Materials and Methods
2.1. Cell Culture and Induction of HL-60 Cell Differentiation
2.2. Incubation of Human Monomeric IgG1, IgG2, and the Fab and Fc Fragments of Human IgG with dHL-60
2.3. Measurement NETs Formation
2.4. Determination of ROS Generation by PMA-Stimulated dHL-60 Cells
2.4.1. Quantification of NADPH/NADP+ Ratio
2.4.2. Measurement of Nitric Oxide
2.4.3. Assessment of the General ROS in Cells
2.5. Western Blot Analysis
2.6. Quantitation of Proinflammatory Cytokines
2.7. Statistical Analyses
3. Results
3.1. PMA-Stimulated NET Formation by dHL-60
3.2. The Effects of Preincubation of dHL-60 with Different Human Monomeric IgG Subclasses (IgG1 and IgG2) and IgG Fragments (Fab and Fc) on PMA-Stimulated NET Formation
3.3. Dose-Dependent Augmenting Effect of Human IgG Fc Receptor Type III (FcγRIII) Engagement on PMA-Stimulated dHL-60 NET Formation
3.4. Increased Intracellular ROS Production and ROS Released from dHL-60 via FcγRIII Engagement
3.4.1. Intracellular NADPH/NADP+ Ratio in dHL-60 and Nitrite in the Culture Supernatants without PMA Stimulation
3.4.2. Increased Intracellular ROS Concentration in dHL-60 Cells by IgG Molecules per se via Fc Region Engagement
3.5. Activation of Syk-ERK Signaling Pathway by IgG Fc Receptor Engagement
3.6. The Release of Proinflammatory Cytokines by dHL-60 Cells
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Lu, C.-H.; Li, K.-J.; Wu, C.-H.; Shen, C.-Y.; Kuo, Y.-M.; Hsieh, S.-C.; Yu, C.-L. The FcγRIII Engagement Augments PMA-Stimulated Neutrophil Extracellular Traps (NETs) Formation by Granulocytes Partially via Cross-Talk between Syk-ERK-NF-κB and PKC-ROS Signaling Pathways. Biomedicines 2021, 9, 1127. https://doi.org/10.3390/biomedicines9091127
Lu C-H, Li K-J, Wu C-H, Shen C-Y, Kuo Y-M, Hsieh S-C, Yu C-L. The FcγRIII Engagement Augments PMA-Stimulated Neutrophil Extracellular Traps (NETs) Formation by Granulocytes Partially via Cross-Talk between Syk-ERK-NF-κB and PKC-ROS Signaling Pathways. Biomedicines. 2021; 9(9):1127. https://doi.org/10.3390/biomedicines9091127
Chicago/Turabian StyleLu, Cheng-Hsun, Ko-Jen Li, Cheng-Han Wu, Chieh-Yu Shen, Yu-Min Kuo, Song-Chou Hsieh, and Chia-Li Yu. 2021. "The FcγRIII Engagement Augments PMA-Stimulated Neutrophil Extracellular Traps (NETs) Formation by Granulocytes Partially via Cross-Talk between Syk-ERK-NF-κB and PKC-ROS Signaling Pathways" Biomedicines 9, no. 9: 1127. https://doi.org/10.3390/biomedicines9091127