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Article

Method Standardization for Conducting Innate Color Preference Studies in Different Zebrafish Strains

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Department of Bioscience Technology, Chung Yuan Christian University, Chung-Li 320314, Taiwan
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Department of Chemistry, Chung Yuan Christian University, Chung-Li 320314, Taiwan
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Department of Chemistry, Chinese Culture University, Taipei 11114, Taiwan
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Department of Biological Science & Technology, College of Medicine, I-Shou University, Kaohsiung 82445, Taiwan
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Department of Applied Chemistry, National Pingtung University, Pingtung 900391, Taiwan
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Center for Nanotechnology, Chung Yuan Christian University, Chung-Li 320314, Taiwan
*
Authors to whom correspondence should be addressed.
These authors contributed equally to this work.
Biomedicines 2020, 8(8), 271; https://doi.org/10.3390/biomedicines8080271
Received: 22 June 2020 / Revised: 28 July 2020 / Accepted: 30 July 2020 / Published: 3 August 2020
(This article belongs to the Special Issue Zebrafish Models for Development and Disease 2.0)
The zebrafish has a tetrachromatic vision that is able to distinguish ultraviolet (UV) and visible wavelengths. Recently, zebrafish color preferences have gained much attention because of the easy setup of the instrument and its usefulness to screen behavior-linked stimuli. However, several published papers dealing with zebrafish color preferences have contradicting results that underscore the importance of method standardization in this field. Different laboratories may report different results because of variations in light source, color intensity, and other parameters such as age, gender, container size, and strain of fish. In this study, we aim to standardize the color preference test in zebrafish by measuring light source position, light intensity, gender, age, animal size to space ratio, and animal strain. Our results showed that color preferences for zebrafish are affected by light position, age, strain, and social interaction of the fish, but not affected by fish gender. We validated that ethanol can significantly induce color preference alteration in zebrafish which may be related to anxiety and depression. We also explored the potential use of the optimized method to examine color preference ranking and index differences in various zebrafish strains and species, such as the tiger barb and glass catfish. In conclusion, zebrafish color preference screening is a powerful tool for high-throughput neuropharmacological applications and the standardized protocol established in this study provides a useful reference for the zebrafish research community. View Full-Text
Keywords: zebrafish behavior; color preferences; toxicity assessment zebrafish behavior; color preferences; toxicity assessment
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MDPI and ACS Style

Siregar, P.; Juniardi, S.; Audira, G.; Lai, Y.-H.; Huang, J.-C.; Chen, K.H.-C.; Chen, J.-R.; Hsiao, C.-D. Method Standardization for Conducting Innate Color Preference Studies in Different Zebrafish Strains. Biomedicines 2020, 8, 271. https://doi.org/10.3390/biomedicines8080271

AMA Style

Siregar P, Juniardi S, Audira G, Lai Y-H, Huang J-C, Chen KH-C, Chen J-R, Hsiao C-D. Method Standardization for Conducting Innate Color Preference Studies in Different Zebrafish Strains. Biomedicines. 2020; 8(8):271. https://doi.org/10.3390/biomedicines8080271

Chicago/Turabian Style

Siregar, Petrus, Stevhen Juniardi, Gilbert Audira, Yu-Heng Lai, Jong-Chin Huang, Kelvin H.-C. Chen, Jung-Ren Chen, and Chung-Der Hsiao. 2020. "Method Standardization for Conducting Innate Color Preference Studies in Different Zebrafish Strains" Biomedicines 8, no. 8: 271. https://doi.org/10.3390/biomedicines8080271

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