The Effectiveness of Multi-Session FMT Treatment in Active Ulcerative Colitis Patients: A Pilot Study

Department of Gastroenterology, Dietetics and Internal Medicine, Poznan University of Medical Sciences, Heliodor Święcicki Hospital, Przybyszewskiego 49, 60-355 Poznań, Poland

Department of Biophysics, Poznan University of Medical Sciences, Grunwaldzka 6, 60-780 Poznań, Poland

Department of Dermatology and Venereology, Poznan University of Medical Sciences, Przybyszewskiego 49, 60-355 Poznań, Poland

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Central Microbiological Laboratory, Święcicki University Hospital, Przybyszewskiego 49, 60-355 Poznań, Poland

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Institute of Human Genetics, Polish Academy of Sciences, Strzeszyńska 32, 60-479 Poznań, Poland

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Department of Computer Science and Statistics, Poznan University of Medical Sciences, Rokietnicka 7, 60-806 Poznań, Poland

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Department of Biochemistry and Biotechnology, University of Life Sciences, Dojazd 11, 60-632 Poznań, Poland

Author to whom correspondence should be addressed.

Biomedicines 2020, 8(8), 268; https://doi.org/10.3390/biomedicines8080268

Received: 8 July 2020 / Revised: 27 July 2020 / Accepted: 29 July 2020 / Published: 3 August 2020

(This article belongs to the Special Issue Microbial Ecology in Health and Disease)

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Abstract

The modification of the microbiome through fecal microbiota transplantation (FMT) is becoming a very promising therapeutic option for inflammatory bowel disease (IBD) patients. Our pilot study aimed to assess the effectiveness of multi-session FMT treatment in active ulcerative colitis (UC) patients. Ten patients with UC were treated with multi-session FMT (200 mL) from healthy donors, via colonoscopy/gastroscopy. Patients were evaluated as follows: at baseline, at week 7, and after 6 months, routine blood tests (including C reactive protein (CRP) and calprotectin) were performed. 16S rRNA gene (V3V4) sequencing was used for metagenomic analysis. The severity of UC was classified based on the Truelove–Witts index. The assessment of microbial diversity showed significant differences between recipients and healthy donors. FMT contributed to long-term, significant clinical and biochemical improvement. Metagenomic analysis revealed an increase in the amount of Lactobacillaceaea, Micrococcaceae, Prevotellaceae, and TM7 phylumsp.oral clone EW055 during FMT, whereas Staphylococcaceae and Bacillaceae declined significantly. A positive increase in the proportion of the genera Bifidobacterium, Lactobacillus, Rothia, Streptococcus, and Veillonella and a decrease in Bacillus, Bacteroides, and Staphylococcus were observed based on the correlation between calprotectin and Bacillus and Staphylococcus; ferritin and Lactobacillus, Veillonella, and Bifidobacterium abundance was indicated. A positive change in the abundance of Firmicutes was observed during FMT and after 6 months. The application of multi-session FMT led to the restoration of recipients’ microbiota and resulted in the remission of patients with active UC. View Full-Text

Keywords: fecal microbiota transplantation (FMT); inflammatory bowel disease (IBD); ulcerative colitis (UC); gut microbiota

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Mańkowska-Wierzbicka, D.; Stelmach-Mardas, M.; Gabryel, M.; Tomczak, H.; Skrzypczak-Zielińska, M.; Zakerska-Banaszak, O.; Sowińska, A.; Mahadea, D.; Baturo, A.; Wolko, Ł.; Słomski, R.; Dobrowolska, A. The Effectiveness of Multi-Session FMT Treatment in Active Ulcerative Colitis Patients: A Pilot Study. Biomedicines 2020, 8, 268. https://doi.org/10.3390/biomedicines8080268

AMA Style

Mańkowska-Wierzbicka D, Stelmach-Mardas M, Gabryel M, Tomczak H, Skrzypczak-Zielińska M, Zakerska-Banaszak O, Sowińska A, Mahadea D, Baturo A, Wolko Ł, Słomski R, Dobrowolska A. The Effectiveness of Multi-Session FMT Treatment in Active Ulcerative Colitis Patients: A Pilot Study. Biomedicines. 2020; 8(8):268. https://doi.org/10.3390/biomedicines8080268

Chicago/Turabian Style

Mańkowska-Wierzbicka, Dorota, Marta Stelmach-Mardas, Marcin Gabryel, Hanna Tomczak, Marzena Skrzypczak-Zielińska, Oliwia Zakerska-Banaszak, Anna Sowińska, Dagmara Mahadea, Alina Baturo, Łukasz Wolko, Ryszard Słomski, and Agnieszka Dobrowolska. 2020. "The Effectiveness of Multi-Session FMT Treatment in Active Ulcerative Colitis Patients: A Pilot Study" Biomedicines 8, no. 8: 268. https://doi.org/10.3390/biomedicines8080268

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