Next Article in Journal
Unraveling the Role of Inwardly Rectifying Potassium Channels in the Hippocampus of an Aβ(1–42)-Infused Rat Model of Alzheimer’s Disease
Previous Article in Journal
The Biological Role of Apurinic/Apyrimidinic Endonuclease1/Redox Factor-1 as a Therapeutic Target for Vascular Inflammation and as a Serologic Biomarker
Article

Characterization of Osteoarthritis in a Medial Meniscectomy-Induced Animal Model Using Contrast-Enhanced X-ray Microtomography

1
Laboratory of Laboratory/Sports Medicine, Division of Clinical Medicine, Faculty of Medicine, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8577, Japan
2
Doctoral Program in Sports Medicine, Graduate School of Comprehensive Human Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8577, Japan
3
Master’s Program in Medical Sciences, Graduate School of Comprehensive Human Sciences, University of Tsukuba, T1-1-1 Tennodai, Tsukuba, Ibaraki 305-8577, Japan
4
Bonding and Manufacturing Field, Research Center for Structural Materials, National Institute for Materials Science, 1-2-1 Sengen, Tsukuba, Ibaraki 305-0047, Japan
5
Research Center for Advanced Science and Technology, The University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo 153-8904, Japan
6
Department of Medical Technology, Faculty of Health Sciences, Tsukuba International University, 6-20-1 Manabe, Tsuchiura, Ibaraki 300-0051, Japan
7
Nutrigenomics Research Group, Faculty of Medicine, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8577, Japan
*
Author to whom correspondence should be addressed.
Theses authors contributed equally to this work.
Biomedicines 2020, 8(3), 56; https://doi.org/10.3390/biomedicines8030056
Received: 8 February 2020 / Revised: 26 February 2020 / Accepted: 3 March 2020 / Published: 10 March 2020
(This article belongs to the Section Molecular and Translational Medicine)
The aim of this study was to clarify degradation characteristics in each tissue of the knee complex of a medial meniscectomy (MMx)-induced knee osteoarthritis (KOA) animal model using classical methods and an alternative comprehensive evaluation method called contrast-enhanced X-ray micro-computed tomography (CEX-μCT), which was developed in the study. Surgical MMx was performed in the right knee joints of five male Wistar rats to induce KOA. At four weeks post-surgery, the synovitis was evaluated using quantitative polymerase chain reaction (qPCR). Degradations of the articular cartilage of the tibial plateau were evaluated using classical methods and CEX-μCT. Evaluation of the synovitis demonstrated significantly increased expression levels of inflammation-associated marker genes in MMx-treated knees compared with those in sham-treated knees. Evaluation of the articular cartilage using classical methods showed that MMx fully induced degradation of the cartilage. Evaluation using CEX-μCT showed that local areas of the medial cartilage of the tibial plateau were significantly reduced in MMx-treated knees compared with those in sham-treated knees. On the other hand, total cartilage volumes were significantly increased in MMx-treated knees. On the basis of the findings of this study, the method could be relevant to study new treatments in KOA research. View Full-Text
Keywords: osteoarthritis; synovitis; articular cartilage; microfocus X-ray CT; 3D analysis osteoarthritis; synovitis; articular cartilage; microfocus X-ray CT; 3D analysis
Show Figures

Graphical abstract

MDPI and ACS Style

Sugasawa, T.; Kuji, T.; Aoki, K.; Yanazawa, K.; Takenouchi, A.; Watanabe, M.; Tome, Y.; Takeuchi, Y.; Aita, Y.; Yahagi, N.; Shishikura, Y.; Ono, S.; Yoshida, Y.; Kawakami, Y.; Takekoshi, K. Characterization of Osteoarthritis in a Medial Meniscectomy-Induced Animal Model Using Contrast-Enhanced X-ray Microtomography. Biomedicines 2020, 8, 56. https://doi.org/10.3390/biomedicines8030056

AMA Style

Sugasawa T, Kuji T, Aoki K, Yanazawa K, Takenouchi A, Watanabe M, Tome Y, Takeuchi Y, Aita Y, Yahagi N, Shishikura Y, Ono S, Yoshida Y, Kawakami Y, Takekoshi K. Characterization of Osteoarthritis in a Medial Meniscectomy-Induced Animal Model Using Contrast-Enhanced X-ray Microtomography. Biomedicines. 2020; 8(3):56. https://doi.org/10.3390/biomedicines8030056

Chicago/Turabian Style

Sugasawa, Takehito, Tomoaki Kuji, Kai Aoki, Koki Yanazawa, Akiko Takenouchi, Makoto Watanabe, Yoshiya Tome, Yoshinori Takeuchi, Yuichi Aita, Naoya Yahagi, Yasuhiro Shishikura, Seiko Ono, Yasuko Yoshida, Yasushi Kawakami, and Kazuhiro Takekoshi. 2020. "Characterization of Osteoarthritis in a Medial Meniscectomy-Induced Animal Model Using Contrast-Enhanced X-ray Microtomography" Biomedicines 8, no. 3: 56. https://doi.org/10.3390/biomedicines8030056

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop