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HSP70 Inhibitor Suppresses IGF-I-Stimulated Migration of Osteoblasts through p44/p42 MAP Kinase

1
Department of Orthopedic Surgery, Nagoya City University Graduate School of Medical Sciences, Nagoya 467-8601, Japan
2
Department of Pharmacology, Gifu University Graduate School of Medicine, Gifu 501-1194, Japan
3
Department of Orthopedic Surgery, Toyokawa City Hospital, Toyokawa 442-8561, Japan
4
Department of Clinical Laboratory, National Center for Geriatrics and Gerontology, Obu 474-8511, Japan
5
Department of Rehabilitation Medicine, Nagoya City University Graduate School of Medical Sciences, Nagoya 467-8601, Japan
*
Author to whom correspondence should be addressed.
Biomedicines 2018, 6(4), 109; https://doi.org/10.3390/biomedicines6040109
Received: 28 August 2018 / Revised: 14 November 2018 / Accepted: 15 November 2018 / Published: 21 November 2018
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Abstract

Heat shock protein 70 (HSP70) is a ubiquitously expressed molecular chaperone in a variety of cells including osteoblasts. We previously showed that insulin-like growth factor-I (IGF-I) elicits migration of osteoblast-like MC3T3-E1 cells through the activation of phosphatidylinositol 3-kinase/Akt and p44/p42 mitogen-activated protein (MAP) kinase. In the present study, we investigated the effects of HSP70 inhibitors on the IGF-I-elicited migration of these cells and the mechanism involved. The IGF-I-stimulated osteoblast migration evaluated by a wound-healing assay and by a transwell cell migration was significantly reduced by VER-155008 and YM-08, which are both HSP70 inhibitors. VER-155008 markedly suppressed the IGF-I-induced phosphorylation of p44/p42 MAP kinase without affecting that of Akt. In conclusion, our results strongly suggest that the HSP70 inhibitor reduces the IGF-I-elicited migration of osteoblasts via the p44/p42 MAP kinase. View Full-Text
Keywords: migration; HSP70 inhibitor; IGF-I; p44/p42 MAP kinase; osteoblast migration; HSP70 inhibitor; IGF-I; p44/p42 MAP kinase; osteoblast
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
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Kawabata, T.; Tokuda, H.; Sakai, G.; Fujita, K.; Matsushima-Nishiwaki, R.; Kuroyanagi, G.; Otsuka, T.; Kozawa, O. HSP70 Inhibitor Suppresses IGF-I-Stimulated Migration of Osteoblasts through p44/p42 MAP Kinase. Biomedicines 2018, 6, 109.

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