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Open AccessArticle

HSP70 Inhibitor Suppresses IGF-I-Stimulated Migration of Osteoblasts through p44/p42 MAP Kinase

1
Department of Orthopedic Surgery, Nagoya City University Graduate School of Medical Sciences, Nagoya 467-8601, Japan
2
Department of Pharmacology, Gifu University Graduate School of Medicine, Gifu 501-1194, Japan
3
Department of Orthopedic Surgery, Toyokawa City Hospital, Toyokawa 442-8561, Japan
4
Department of Clinical Laboratory, National Center for Geriatrics and Gerontology, Obu 474-8511, Japan
5
Department of Rehabilitation Medicine, Nagoya City University Graduate School of Medical Sciences, Nagoya 467-8601, Japan
*
Author to whom correspondence should be addressed.
Biomedicines 2018, 6(4), 109; https://doi.org/10.3390/biomedicines6040109
Received: 28 August 2018 / Revised: 14 November 2018 / Accepted: 15 November 2018 / Published: 21 November 2018
Heat shock protein 70 (HSP70) is a ubiquitously expressed molecular chaperone in a variety of cells including osteoblasts. We previously showed that insulin-like growth factor-I (IGF-I) elicits migration of osteoblast-like MC3T3-E1 cells through the activation of phosphatidylinositol 3-kinase/Akt and p44/p42 mitogen-activated protein (MAP) kinase. In the present study, we investigated the effects of HSP70 inhibitors on the IGF-I-elicited migration of these cells and the mechanism involved. The IGF-I-stimulated osteoblast migration evaluated by a wound-healing assay and by a transwell cell migration was significantly reduced by VER-155008 and YM-08, which are both HSP70 inhibitors. VER-155008 markedly suppressed the IGF-I-induced phosphorylation of p44/p42 MAP kinase without affecting that of Akt. In conclusion, our results strongly suggest that the HSP70 inhibitor reduces the IGF-I-elicited migration of osteoblasts via the p44/p42 MAP kinase. View Full-Text
Keywords: migration; HSP70 inhibitor; IGF-I; p44/p42 MAP kinase; osteoblast migration; HSP70 inhibitor; IGF-I; p44/p42 MAP kinase; osteoblast
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MDPI and ACS Style

Kawabata, T.; Tokuda, H.; Sakai, G.; Fujita, K.; Matsushima-Nishiwaki, R.; Kuroyanagi, G.; Otsuka, T.; Kozawa, O. HSP70 Inhibitor Suppresses IGF-I-Stimulated Migration of Osteoblasts through p44/p42 MAP Kinase. Biomedicines 2018, 6, 109. https://doi.org/10.3390/biomedicines6040109

AMA Style

Kawabata T, Tokuda H, Sakai G, Fujita K, Matsushima-Nishiwaki R, Kuroyanagi G, Otsuka T, Kozawa O. HSP70 Inhibitor Suppresses IGF-I-Stimulated Migration of Osteoblasts through p44/p42 MAP Kinase. Biomedicines. 2018; 6(4):109. https://doi.org/10.3390/biomedicines6040109

Chicago/Turabian Style

Kawabata, Tetsu; Tokuda, Haruhiko; Sakai, Go; Fujita, Kazuhiko; Matsushima-Nishiwaki, Rie; Kuroyanagi, Gen; Otsuka, Takanobu; Kozawa, Osamu. 2018. "HSP70 Inhibitor Suppresses IGF-I-Stimulated Migration of Osteoblasts through p44/p42 MAP Kinase" Biomedicines 6, no. 4: 109. https://doi.org/10.3390/biomedicines6040109

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