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Comparison of Synthetic Media Designed for Expansion of Adipose-Derived Mesenchymal Stromal Cells

Biorepository, University of Arizona, AHSC 6122, P.O. Box 245221, Tucson, AZ 85724, USA
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Biomedicines 2018, 6(2), 54; https://doi.org/10.3390/biomedicines6020054
Received: 18 April 2018 / Revised: 8 May 2018 / Accepted: 9 May 2018 / Published: 14 May 2018
Mesenchymal stromal cells (MSCs) are multipotent cells that can differentiate into various cell types, such as osteoblasts, myocytes, and adipocytes. This characteristic makes the cells a useful tool in developing new therapies for a number of common maladies and diseases. The utilization of animal-derived growth serum, such as fetal bovine serum (FBS), for the expansion of MSCs has traditionally been used for cell culture. However, in clinical applications, animal-derived products present limitations and safety concerns for the recipient, as exposure to animal (xeno-) antigens and infectious agents is possible. Multiple synthetic, xeno-free media have been developed to combat these limitations of animal-derived growth serum and have the potential to be used in ex vivo MSC expansion for clinical use. The goal of this study was to determine if xeno-free media are adequate to significantly and efficiently expand MSCs derived from adipose tissue. MSCs were cultured in both standard FBS-containing as well as xeno-free media. The media were compared for cell yield, viability, and phenotypic expression via flow cytometry and directed differentiation. The xeno-free media that were tested were StemMACS MSC Expansion Media (Miltenyi Biotec, Bergisch Gladbach, Germany), PLTMax Human Platelet Lysate (Sigma-Aldrich, St. Louis, MO, USA), and MesenCult-hPL media (Stemcell Technologies, Vancouver, BC, Canada). All xeno-free media showed promise as a feasible replacement for animal-derived growth serums. The xeno-free media expanded MSCs more quickly than the FBS-containing medium and also showed great similarity in cell viability and phenotypic expression. In fact, each xeno-free media produced a greater viable cell yield than the standard FBS-containing medium. View Full-Text
Keywords: stem cells; MSC; tissue engineering; regenerative medicine; expansion; synthetic media stem cells; MSC; tissue engineering; regenerative medicine; expansion; synthetic media
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MDPI and ACS Style

Lensch, M.; Muise, A.; White, L.; Badowski, M.; Harris, D. Comparison of Synthetic Media Designed for Expansion of Adipose-Derived Mesenchymal Stromal Cells. Biomedicines 2018, 6, 54. https://doi.org/10.3390/biomedicines6020054

AMA Style

Lensch M, Muise A, White L, Badowski M, Harris D. Comparison of Synthetic Media Designed for Expansion of Adipose-Derived Mesenchymal Stromal Cells. Biomedicines. 2018; 6(2):54. https://doi.org/10.3390/biomedicines6020054

Chicago/Turabian Style

Lensch, Michelle, Angela Muise, Lisa White, Michael Badowski, and David Harris. 2018. "Comparison of Synthetic Media Designed for Expansion of Adipose-Derived Mesenchymal Stromal Cells" Biomedicines 6, no. 2: 54. https://doi.org/10.3390/biomedicines6020054

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