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Biomedicines 2016, 4(2), 9;

Genetic Modification of T Cells

Bluebird bio, 150 Second Street, Cambridge, MA 02141, USA
Author to whom correspondence should be addressed.
Academic Editor: Vincenzo Cerullo
Received: 27 February 2016 / Revised: 11 April 2016 / Accepted: 13 April 2016 / Published: 20 April 2016
(This article belongs to the Section Tumor Immunology and Immunotherapy)
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Gene transfer technology and its application to human gene therapy greatly expanded in the last decade. One area of investigation that appears particularly promising is the transfer of new genetic material into T cells for the potential treatment of cancer. Herein, we describe several core technologies that now yield high-efficiency gene transfer into primary human T cells. These gene transfer techniques include viral-based gene transfer methods based on modified Retroviridae and non-viral methods such as DNA-based transposons and direct transfer of mRNA by electroporation. Where specific examples are cited, we emphasize the transfer of chimeric antigen receptors (CARs) to T cells, which permits engineered T cells to recognize potential tumor antigens. View Full-Text
Keywords: CAR (chimeric antigen receptor) T cells; immunotherapy; retroviral vector; lentiviral vector; CD19 CAR CAR (chimeric antigen receptor) T cells; immunotherapy; retroviral vector; lentiviral vector; CD19 CAR

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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

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Morgan, R.A.; Boyerinas, B. Genetic Modification of T Cells. Biomedicines 2016, 4, 9.

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