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Volume 13
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10.3390/biomedicines13081865
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Article

Daily Profile of miRNAs in the Rat Colon and In Silico Analysis of Their Possible Relationship to Colorectal Cancer

by
Iveta Herichová
1,*,
Denisa Vanátová
1,
Richard Reis
2,
Katarína Stebelová
1,
Lucia Olexová
1,
Martina Morová
1,
Adhideb Ghosh
3,
Miroslav Baláž
1,3,4,
Peter Štefánik
1 and
Lucia Kršková
1
1
Department of Animal Physiology and Ethology, Faculty of Natural Sciences, Comenius University in Bratislava, 842 15 Bratislava, Slovakia
2
First Surgery Department, University Hospital, Comenius University in Bratislava, 811 07 Bratislava, Slovakia
3
Institute of Food, Nutrition and Health, ETH Zürich, 8603 Schwerzenbach, Switzerland
4
Biomedical Research Center, Slovak Academy of Sciences, 845 05 Bratislava, Slovakia
*
Author to whom correspondence should be addressed.
Biomedicines 2025, 13(8), 1865; https://doi.org/10.3390/biomedicines13081865
Submission received: 2 July 2025 / Revised: 27 July 2025 / Accepted: 29 July 2025 / Published: 31 July 2025
(This article belongs to the Section Molecular Genetics and Genetic Diseases)
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Abstract

Background: Colorectal cancer (CRC) is strongly influenced by miRNAs as well as the circadian system. Methods: High-throughput sequencing of miRNAs expressed in the rat colon during 24 h light (L)/dark (D) cycle was performed to identify rhythmically expressed miRNAs. The role of miR-150-5p in CRC progression was analyzed in DLD1 cell line and human CRC tissues. Results: Nearly 10% of mature miRNAs showed a daily rhythm in expression. A peak of miRNAs’ levels was in most cases observed during the first half of the D phase of the LD cycle. The highest amplitude was detected in expression of miR-150-5p and miR-142-3p. In the L phase of the LD cycle, the maximum in miR-30d-5p expression was detected. Gene ontology enrichment analysis revealed that genes interfering with miRNAs with peak expression during the D phase influence apoptosis, angiogenesis, the immune system, and EGF and TGF-beta signaling. Rhythm in miR-150-5p, miR-142-3p, and miR-30d-5p expression was confirmed by real-time PCR. Oncogenes bcl2 and myb and clock gene cry1 were identified as miR-150-5p targets. miR-150-5p administration promoted camptothecin-induced apoptosis. Expression of myb showed a rhythmic profile in DLD1 cells with inverted acrophase with respect to miR-150-5p. miR-150-5p was decreased in cancer compared to adjacent tissue in CRC patients. Decrease in miR-150-5p was age dependent. Older patients with lower expression of miR-150-5p and higher expression of cry1 showed worse survival in comparison with younger patients. Conclusions: miRNA signaling differs between the L and D phases of the LD cycle. miR-150-5p, targeting myb, bcl2, and cry1, can influence CRC progression in a phase-dependent manner.
Keywords: miR-150; miR-30d; miR-142; bcl2; myb; cry1; circadian; DGCR8; age-dependent miR-150; miR-30d; miR-142; bcl2; myb; cry1; circadian; DGCR8; age-dependent
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MDPI and ACS Style

Herichová, I.; Vanátová, D.; Reis, R.; Stebelová, K.; Olexová, L.; Morová, M.; Ghosh, A.; Baláž, M.; Štefánik, P.; Kršková, L. Daily Profile of miRNAs in the Rat Colon and In Silico Analysis of Their Possible Relationship to Colorectal Cancer. Biomedicines 2025, 13, 1865. https://doi.org/10.3390/biomedicines13081865

AMA Style

Herichová I, Vanátová D, Reis R, Stebelová K, Olexová L, Morová M, Ghosh A, Baláž M, Štefánik P, Kršková L. Daily Profile of miRNAs in the Rat Colon and In Silico Analysis of Their Possible Relationship to Colorectal Cancer. Biomedicines. 2025; 13(8):1865. https://doi.org/10.3390/biomedicines13081865

Chicago/Turabian Style

Herichová, Iveta, Denisa Vanátová, Richard Reis, Katarína Stebelová, Lucia Olexová, Martina Morová, Adhideb Ghosh, Miroslav Baláž, Peter Štefánik, and Lucia Kršková. 2025. "Daily Profile of miRNAs in the Rat Colon and In Silico Analysis of Their Possible Relationship to Colorectal Cancer" Biomedicines 13, no. 8: 1865. https://doi.org/10.3390/biomedicines13081865

APA Style

Herichová, I., Vanátová, D., Reis, R., Stebelová, K., Olexová, L., Morová, M., Ghosh, A., Baláž, M., Štefánik, P., & Kršková, L. (2025). Daily Profile of miRNAs in the Rat Colon and In Silico Analysis of Their Possible Relationship to Colorectal Cancer. Biomedicines, 13(8), 1865. https://doi.org/10.3390/biomedicines13081865

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