Next Article in Journal
Breast Cancer Molecular Subtype Prediction: A Mammography-Based AI Approach
Previous Article in Journal
Immune Responses to Mycobacterium tuberculosis Infection in the Liver of Diabetic Mice
 
 
Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
Journals
Biomedicines
Volume 12
Issue 6
10.3390/biomedicines12061369
biomedicines-logo
Submit to this Journal Review for this Journal Propose a Special Issue
► Article Menu

Article Menu

share Share announcement Help format_quote Cite question_answer Discuss in SciProfiles
first_page
settings
Order Article Reprints
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
Review

Therapeutic Efficacy of Interferon-Gamma and Hypoxia-Primed Mesenchymal Stromal Cells and Their Extracellular Vesicles: Underlying Mechanisms and Potentials in Clinical Translation

by
Yu Ling Tan
1,
Maimonah Eissa Al-Masawa
1,
Sue Ping Eng
2,
Mohamad Nasir Shafiee
3,
Jia Xian Law
1 and
Min Hwei Ng
1,*
1
Centre for Tissue Engineering and Regenerative Medicine, Faculty of Medicine, Universiti Kebangsaan Malaysia, Jalan Yaacob Latif, Bandar Tun Razak, Kuala Lumpur 56000, Malaysia
2
NK Biocell Sdn. Bhd, Unit 1-22A, 1st Floor Pusat Perdagangan Berpadu (United Point), No.10, Jalan Lang Emas, Kuala Lumpur 51200, Malaysia
3
Department of Obstetrics & Gynaecology, Faculty of Medicine, Universiti Kebangsaan Malaysia, Kuala Lumpur 56000, Malaysia
*
Author to whom correspondence should be addressed.
Biomedicines 2024, 12(6), 1369; https://doi.org/10.3390/biomedicines12061369
Submission received: 17 April 2024 / Revised: 31 May 2024 / Accepted: 6 June 2024 / Published: 20 June 2024
(This article belongs to the Section Gene and Cell Therapy)
Browse Figures
Review Reports Versions Notes

Abstract

Multipotent mesenchymal stromal cells (MSCs) hold promises for cell therapy and tissue engineering due to their self-renewal and differentiation abilities, along with immunomodulatory properties and trophic factor secretion. Extracellular vesicles (EVs) from MSCs offer similar therapeutic effects. However, MSCs are heterogeneous and lead to variable outcomes. In vitro priming enhances MSC performance, improving immunomodulation, angiogenesis, proliferation, and tissue regeneration. Various stimuli, such as cytokines, growth factors, and oxygen tension, can prime MSCs. Two classical priming methods, interferon-gamma (IFN-γ) and hypoxia, enhance MSC immunomodulation, although standardized protocols are lacking. This review discusses priming protocols, highlighting the most commonly used concentrations and durations, along with mechanisms and in vivo therapeutics effects of primed MSCs and their EVs. The feasibility of up-scaling their production was also discussed. The review concluded that priming with IFN-γ or hypoxia (alone or in combination with other factors) boosted the immunomodulation capability of MSCs and their EVs, primarily via the JAK/STAT and PI3K/AKT and Leptin/JAK/STAT and TGF-β/Smad signalling pathways, respectively. Incorporating priming in MSC and EV production enables translation into cell-based or cell-free therapies for various disorders.
Keywords: mesenchymal stromal cells; extracellular vesicles; priming; interferon-gamma; hypoxia; pre-clinical mesenchymal stromal cells; extracellular vesicles; priming; interferon-gamma; hypoxia; pre-clinical

Share and Cite

MDPI and ACS Style

Tan, Y.L.; Al-Masawa, M.E.; Eng, S.P.; Shafiee, M.N.; Law, J.X.; Ng, M.H. Therapeutic Efficacy of Interferon-Gamma and Hypoxia-Primed Mesenchymal Stromal Cells and Their Extracellular Vesicles: Underlying Mechanisms and Potentials in Clinical Translation. Biomedicines 2024, 12, 1369. https://doi.org/10.3390/biomedicines12061369

AMA Style

Tan YL, Al-Masawa ME, Eng SP, Shafiee MN, Law JX, Ng MH. Therapeutic Efficacy of Interferon-Gamma and Hypoxia-Primed Mesenchymal Stromal Cells and Their Extracellular Vesicles: Underlying Mechanisms and Potentials in Clinical Translation. Biomedicines. 2024; 12(6):1369. https://doi.org/10.3390/biomedicines12061369

Chicago/Turabian Style

Tan, Yu Ling, Maimonah Eissa Al-Masawa, Sue Ping Eng, Mohamad Nasir Shafiee, Jia Xian Law, and Min Hwei Ng. 2024. "Therapeutic Efficacy of Interferon-Gamma and Hypoxia-Primed Mesenchymal Stromal Cells and Their Extracellular Vesicles: Underlying Mechanisms and Potentials in Clinical Translation" Biomedicines 12, no. 6: 1369. https://doi.org/10.3390/biomedicines12061369

APA Style

Tan, Y. L., Al-Masawa, M. E., Eng, S. P., Shafiee, M. N., Law, J. X., & Ng, M. H. (2024). Therapeutic Efficacy of Interferon-Gamma and Hypoxia-Primed Mesenchymal Stromal Cells and Their Extracellular Vesicles: Underlying Mechanisms and Potentials in Clinical Translation. Biomedicines, 12(6), 1369. https://doi.org/10.3390/biomedicines12061369

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

Article Metrics

Back to TopTop