BZD9L1 Differentially Regulates Sirtuins in Liver-Derived Cells by Inducing Reactive Oxygen Species
Abstract
:1. Introduction
2. Materials and Methods
2.1. Cell Culture
2.2. Cell Treatment
2.3. Cell Viability Assay
2.4. ROS Assay
2.5. Mito Stress Test Assay
2.6. Western Blot
2.7. Statistical Analyses
3. Results
3.1. BZD9L1 Reduced THLE-2 and HEK293 Cell Viability but Induced ROS Only in THLE-2 Cells
3.2. Effects of BZD9L1 on the Bioenergetics Profile of THLE-2 and HEK293 Cells
3.3. Effects of BZD9L1 on the Regulation of Sirtuins in THLE-2 and HEK293 Cells
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Conflicts of Interest
References
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Treatment Duration | Cell Viability (IC50), µM | |
---|---|---|
THLE-2 | HEK293 | |
24 h | 178.92 ± 0.049 | 77.72 ± 0.083 |
48 h | 21.11 ± 0.052 | 31.00 ± 0.089 |
72 h | 15.75 ± 0.051 | 23.74 ± 0.072 |
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Lee, Y.T.; Tan, Y.J.; Oon, C.E. BZD9L1 Differentially Regulates Sirtuins in Liver-Derived Cells by Inducing Reactive Oxygen Species. Biomedicines 2023, 11, 3059. https://doi.org/10.3390/biomedicines11113059
Lee YT, Tan YJ, Oon CE. BZD9L1 Differentially Regulates Sirtuins in Liver-Derived Cells by Inducing Reactive Oxygen Species. Biomedicines. 2023; 11(11):3059. https://doi.org/10.3390/biomedicines11113059
Chicago/Turabian StyleLee, Yeuan Ting, Yi Jer Tan, and Chern Ein Oon. 2023. "BZD9L1 Differentially Regulates Sirtuins in Liver-Derived Cells by Inducing Reactive Oxygen Species" Biomedicines 11, no. 11: 3059. https://doi.org/10.3390/biomedicines11113059