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Article

Neurodynamic Treatment Promotes Mechanical Pain Modulation in Sensory Neurons and Nerve Regeneration in Rats

1
Department of Clinical and Biological Sciences, University of Torino, 10043 Torino, Italy
2
Neuroscience Institute Cavalieri Ottolenghi (NICO), University of Torino, 10043 Torino, Italy
3
Department of Rehabilitation, ASST (Azienda Socio Sanitaria Territoriali) Nord Milano, Sesto San Giovanni Hospital, Sesto San Giovanni, 20099 Milano, Italy
4
Department of Life Sciences and Systems Biology, University of Torino, 10124 Torino, Italy
*
Author to whom correspondence should be addressed.
Academic Editor: Alaa Abd-Elsayed
Biomedicines 2022, 10(6), 1296; https://doi.org/10.3390/biomedicines10061296
Received: 13 May 2022 / Revised: 24 May 2022 / Accepted: 29 May 2022 / Published: 31 May 2022
(This article belongs to the Special Issue Peripheral Nerve Stimulation)
Background: Somatic nerve injuries are a rising problem leading to disability associated with neuropathic pain commonly reported as mechanical allodynia (MA) and hyperalgesia. These symptoms are strongly dependent on specific processes in the dorsal root ganglia (DRG). Neurodynamic treatment (NDT), consisting of selective uniaxial nerve repeated tension protocols, effectively reduces pain and disability in neuropathic pain patients even though the biological mechanisms remain poorly characterized. We aimed to define, both in vivo and ex vivo, how NDT could promote nerve regeneration and modulate some processes in the DRG linked to MA and hyperalgesia. Methods: We examined in Wistar rats, after unilateral median and ulnar nerve crush, the therapeutic effects of NDT and the possible protective effects of NDT administered for 10 days before the injury. We adopted an ex vivo model of DRG organotypic explant subjected to NDT to explore the selective effects on DRG cells. Results: Behavioural tests, morphological and morphometrical analyses, and gene and protein expression analyses were performed, and these tests revealed that NDT promotes nerve regeneration processes, speeds up sensory motor recovery, and modulates mechanical pain by affecting, in the DRG, the expression of TACAN, a mechanosensitive receptor shared between humans and rats responsible for MA and hyperalgesia. The ex vivo experiments have shown that NDT increases neurite regrowth and confirmed the modulation of TACAN. Conclusions: The results obtained in this study on the biological and molecular mechanisms induced by NDT will allow the exploration, in future clinical trials, of its efficacy in different conditions of neuropathic pain. View Full-Text
Keywords: neuropathic pain; neurodynamic; nociceptor; allodynia; regeneration; motor recovery; non-pharmacological treatment; dorsal root ganglia; gene expression neuropathic pain; neurodynamic; nociceptor; allodynia; regeneration; motor recovery; non-pharmacological treatment; dorsal root ganglia; gene expression
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MDPI and ACS Style

Carta, G.; Fornasari, B.E.; Fregnan, F.; Ronchi, G.; De Zanet, S.; Muratori, L.; Nato, G.; Fogli, M.; Gambarotta, G.; Geuna, S.; Raimondo, S. Neurodynamic Treatment Promotes Mechanical Pain Modulation in Sensory Neurons and Nerve Regeneration in Rats. Biomedicines 2022, 10, 1296. https://doi.org/10.3390/biomedicines10061296

AMA Style

Carta G, Fornasari BE, Fregnan F, Ronchi G, De Zanet S, Muratori L, Nato G, Fogli M, Gambarotta G, Geuna S, Raimondo S. Neurodynamic Treatment Promotes Mechanical Pain Modulation in Sensory Neurons and Nerve Regeneration in Rats. Biomedicines. 2022; 10(6):1296. https://doi.org/10.3390/biomedicines10061296

Chicago/Turabian Style

Carta, Giacomo, Benedetta Elena Fornasari, Federica Fregnan, Giulia Ronchi, Stefano De Zanet, Luisa Muratori, Giulia Nato, Marco Fogli, Giovanna Gambarotta, Stefano Geuna, and Stefania Raimondo. 2022. "Neurodynamic Treatment Promotes Mechanical Pain Modulation in Sensory Neurons and Nerve Regeneration in Rats" Biomedicines 10, no. 6: 1296. https://doi.org/10.3390/biomedicines10061296

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