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Article

Biofunctionalization of Xenogeneic Collagen Membranes with Autologous Platelet Concentrate—Influence on Rehydration Protocol and Angiogenesis

1
Department of Oral and Maxillofacial Surgery—Plastic Operations, University Medical Center Mainz, Augustusplatz 2, 55131 Mainz, Germany
2
Department of Oral and Maxillofacial Surgery, Federal Armed Forces Hospital, Rübenacherstr. 170, 56072 Koblenz, Germany
*
Author to whom correspondence should be addressed.
Keyvan Sagheb and Bilal Al-Nawas share senior authorship.
Academic Editor: Célia F. Rodrigues
Biomedicines 2022, 10(3), 706; https://doi.org/10.3390/biomedicines10030706
Received: 11 February 2022 / Revised: 5 March 2022 / Accepted: 14 March 2022 / Published: 18 March 2022
Background: The aim of this study was to analyze possible interactions of different xenogeneic collagen membranes (CM) and platelet-rich fibrin (PRF). PH values were evaluated in the CM rehydration process with PRF, and their influence on angiogenesis was analyzed in vivo. Materials and Methods: Porcine (Bio-Gide®, Geistlich)- and bovine-derived collagen membranes (Symbios®, Dentsply Sirona) were biofunctionalized with PRF by plotting process. PRF in comparison to blood, saline and a puffer pH7 solution was analysed for pH-value changes in CM rehydration process in vitro. The yolk sac membrane (YSM) model was used to investigate pro-angiogenic effects of the combination of PRF and the respective CM in comparison to native pendant by vessel in-growth and branching points after 24, 48 and 72 h evaluated light-microscopically and by immunohistochemical staining (CD105, αSMA) in vivo. Results: Significantly higher pH values were found at all points in time in PRF alone and its combined variants with Bio-Gide® and Symbios® compared with pure native saline solution and pH 7 solution, as well as saline with Symbios® and Bio-Gide® (each p < 0.01). In the YSM, vessel number and branching points showed no significant differences at 24 and 48 h between all groups (each p > 0.05). For PRF alone, a significantly increased vessel number and branching points between 24 and 48 h (each p < 0.05) and between 24 and 72 h (each p < 0.05) was shown. After 72 h, CM in combination with PRF induced a statistically significant addition to vessels and branching points in comparison with native YSM (p < 0.01) but not vs. its native pendants (p > 0.05). Summary: PRF represents a promising alternative for CM rehydration to enhance CM vascularization. View Full-Text
Keywords: platelet-rich fibrin; collagen matrix; in ovo yolk sac; angiogenesis; pH value; guided bone regeneration platelet-rich fibrin; collagen matrix; in ovo yolk sac; angiogenesis; pH value; guided bone regeneration
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MDPI and ACS Style

Blatt, S.; Schröger, S.-V.; Pabst, A.; Kämmerer, P.W.; Sagheb, K.; Al-Nawas, B. Biofunctionalization of Xenogeneic Collagen Membranes with Autologous Platelet Concentrate—Influence on Rehydration Protocol and Angiogenesis. Biomedicines 2022, 10, 706. https://doi.org/10.3390/biomedicines10030706

AMA Style

Blatt S, Schröger S-V, Pabst A, Kämmerer PW, Sagheb K, Al-Nawas B. Biofunctionalization of Xenogeneic Collagen Membranes with Autologous Platelet Concentrate—Influence on Rehydration Protocol and Angiogenesis. Biomedicines. 2022; 10(3):706. https://doi.org/10.3390/biomedicines10030706

Chicago/Turabian Style

Blatt, Sebastian, Saskia-Vanessa Schröger, Andreas Pabst, Peer W. Kämmerer, Keyvan Sagheb, and Bilal Al-Nawas. 2022. "Biofunctionalization of Xenogeneic Collagen Membranes with Autologous Platelet Concentrate—Influence on Rehydration Protocol and Angiogenesis" Biomedicines 10, no. 3: 706. https://doi.org/10.3390/biomedicines10030706

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