Next Article in Journal
Platelets’ Role in Dentistry: From Oral Pathology to Regenerative Potential
Next Article in Special Issue
Inhibitor of Hyaluronic Acid Synthesis 4-Methylumbelliferone Suppresses the Secretory Processes That Ensure the Invasion of Neutrophils into Tissues and Induce Inflammation
Previous Article in Journal
Proteomic Analysis of Mouse Kidney Tissue Associates Peroxisomal Dysfunction with Early Diabetic Kidney Disease
Previous Article in Special Issue
Galectin-9 Triggers Neutrophil-Mediated Anticancer Immunity
Article

Association of Lipoproteins with Neutrophil Extracellular Traps in Patients with Abdominal Aortic Aneurysm

1
Division of Vascular Surgery, Department of General Surgery, Medical University of Vienna, Vienna General Hospital, 1090 Vienna, Austria
2
Department of Laboratory Medicine, Medical University of Vienna, Vienna General Hospital, 1090 Vienna, Austria
3
Center for Pathobiochemistry and Genetics, Department of Medical Chemistry, Medical University of Vienna, 1090 Vienna, Austria
*
Author to whom correspondence should be addressed.
Academic Editor: Galina F. Sud’ina
Biomedicines 2022, 10(2), 217; https://doi.org/10.3390/biomedicines10020217
Received: 9 December 2021 / Revised: 15 January 2022 / Accepted: 17 January 2022 / Published: 20 January 2022
(This article belongs to the Special Issue Neutrophils, Fast and Strong)
Neutrophil extracellular traps (NETs) are DNA–protein structures released by neutrophils in response to various stimuli, including oxidized, low-density lipoprotein (oxLDL). Accumulating evidence suggests a role for NETs in the pathogenesis of abdominal aortic aneurysm (AAA). In this study, we investigated the potential association of lipoprotein particles and NETs in AAA in comparison to non-AAA control groups. The concentrations of neutrophil myeloperoxidase (MPO), the NET parameters citrullinated histone H3 (citH3) and circulating cell-free DNA (cfDNA), as well as of blood lipids were determined in plasma or serum of patients with AAA (n = 40), peripheral artery occlusive disease (PAD; n = 40) and healthy donors (n = 29). A sandwich ELISA detecting oxidized phosphatidylcholine in association with apolipoprotein B-100 (oxPL/apoB) was applied to measure oxidized phospholipids in circulation. The effect of lipoparticles on NET formation was tested using a DNA release assay with isolated human neutrophils. Plasma MPO, citH3 and cfDNA levels were significantly increased in AAA patients in comparison to healthy donors and PAD patients. Plasma concentrations of citH3 positively correlated with serum oxPL/apoB in AAA patients. In functional in vitro assays, the addition of oxLDL induced NET formation in pre-stimulated neutrophils. In conclusion, our data suggest a promoting role of oxLDL on NET formation in AAA patients. View Full-Text
Keywords: abdominal aortic aneurysm; citrullinated histones; lipoproteins; neutrophil extracellular traps; peripheral arterial occlusive disease abdominal aortic aneurysm; citrullinated histones; lipoproteins; neutrophil extracellular traps; peripheral arterial occlusive disease
Show Figures

Figure 1

MDPI and ACS Style

Brandau, A.; Ibrahim, N.; Klopf, J.; Hayden, H.; Ozsvar-Kozma, M.; Afonyushkin, T.; Bleichert, S.; Fuchs, L.; Watzinger, V.; Nairz, V.; Manville, E.; Kessler, V.; Stangl, H.; Eilenberg, W.; Neumayer, C.; Brostjan, C. Association of Lipoproteins with Neutrophil Extracellular Traps in Patients with Abdominal Aortic Aneurysm. Biomedicines 2022, 10, 217. https://doi.org/10.3390/biomedicines10020217

AMA Style

Brandau A, Ibrahim N, Klopf J, Hayden H, Ozsvar-Kozma M, Afonyushkin T, Bleichert S, Fuchs L, Watzinger V, Nairz V, Manville E, Kessler V, Stangl H, Eilenberg W, Neumayer C, Brostjan C. Association of Lipoproteins with Neutrophil Extracellular Traps in Patients with Abdominal Aortic Aneurysm. Biomedicines. 2022; 10(2):217. https://doi.org/10.3390/biomedicines10020217

Chicago/Turabian Style

Brandau, Annika, Nahla Ibrahim, Johannes Klopf, Hubert Hayden, Maria Ozsvar-Kozma, Taras Afonyushkin, Sonja Bleichert, Lukas Fuchs, Viktoria Watzinger, Verena Nairz, Emely Manville, Veronika Kessler, Herbert Stangl, Wolf Eilenberg, Christoph Neumayer, and Christine Brostjan. 2022. "Association of Lipoproteins with Neutrophil Extracellular Traps in Patients with Abdominal Aortic Aneurysm" Biomedicines 10, no. 2: 217. https://doi.org/10.3390/biomedicines10020217

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop