OGG1 Inhibition Reduces Acinar Cell Injury in a Mouse Model of Acute Pancreatitis
Abstract
:1. Introduction
2. Materials and Methods
2.1. Reagents
2.2. Acute Pancreatitis Model
2.3. Isolation and Culture of Pancreatic Acinar Cells
2.4. Serum α-Amylase and Lipase Determination
2.5. Myeloperoxidase Assay
2.6. Histology
2.7. Immunohistochemistry
2.8. Immunofluorescent Staining
2.9. Cytokine Array
2.10. RNA Isolation and Quantitative Real-Time PCR
2.11. Electrophoretic Mobility Shift Assay (EMSA)
2.12. Apoptosis, Necrosis and Viability Assays
2.13. Comet Assay
2.14. Statistical Analysis
3. Results
4. Discussion
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Gene | Forward Primers (5→3) | Reverse Primers (5→3) |
---|---|---|
CCL2 | CACTCACCTGCTGCTACTCA | GCTTGGTGACAAAAACTACAGC |
CCL4 | CCCACTTCCTGCTGTTTCTCT | GCCTCTTTTGGTCAGGAATACCA |
CCL12 | AAGCAGAAGATTCACGTCCG | ATCCAGTATGGTCCTGAAGATCAC |
IL23 | CCAGCGGGACATATGAATCTAC | TGTCCTTGAGTCCTTGTGGG |
IL16 | AACTCCTCTACTGACTCCGC | ACCCTGTTCTGTCCCTTTGA |
IL10 | GCTGTCATCGATTTCTCCCCT | GACACCTTGGTCTTGGAGCTTAT |
CSF1 | GCCCTTCTTCGACATGGCT | CCTTCAGGTGTCCATTCCCA |
TREM1 | TGCCACTTCATACACTGGGT | ATTGTGGAGAAGTCGAGGCA |
IL1-ra | CATTGCTGGGTACTTACAAGGA | AGTGATGTTAACTTCCTCCAGC |
IL6 | TTCTTGGGACTGATGCTGGT | CCATTGCACAACTCTTTTCTCA |
IL1β | TGCCACCTTTTGACAGTGATG | ATGTGCTGCTGCGAGATTTG |
TNFα | GGATGAGAAGTTCCCAAATGGC | TGTCTTTGAGATCCATGCCG |
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Hajnády, Z.; Nagy-Pénzes, M.; Demény, M.A.; Kovács, K.; El-Hamoly, T.; Maléth, J.; Hegyi, P.; Polgár, Z.; Hegedűs, C.; Virág, L. OGG1 Inhibition Reduces Acinar Cell Injury in a Mouse Model of Acute Pancreatitis. Biomedicines 2022, 10, 2543. https://doi.org/10.3390/biomedicines10102543
Hajnády Z, Nagy-Pénzes M, Demény MA, Kovács K, El-Hamoly T, Maléth J, Hegyi P, Polgár Z, Hegedűs C, Virág L. OGG1 Inhibition Reduces Acinar Cell Injury in a Mouse Model of Acute Pancreatitis. Biomedicines. 2022; 10(10):2543. https://doi.org/10.3390/biomedicines10102543
Chicago/Turabian StyleHajnády, Zoltán, Máté Nagy-Pénzes, Máté A. Demény, Katalin Kovács, Tarek El-Hamoly, József Maléth, Péter Hegyi, Zsuzsanna Polgár, Csaba Hegedűs, and László Virág. 2022. "OGG1 Inhibition Reduces Acinar Cell Injury in a Mouse Model of Acute Pancreatitis" Biomedicines 10, no. 10: 2543. https://doi.org/10.3390/biomedicines10102543