Association of Circular RNA and Long Non-Coding RNA Dysregulation with the Clinical Response to Immune Checkpoint Blockade in Cutaneous Metastatic Melanoma
Abstract
:1. Introduction
2. Materials and Methods
2.1. Subjects
2.2. Nucleic Acid Isolation
2.3. Next Generation Sequencing
2.4. Realtime PCR Validation
2.5. lncRNA and circRNA Detection
2.6. Differential Expression Analysis
2.7. ceRNAs-miRNAs-mRNAs Interactions
2.8. Gene Set Enrichment and Gene Interactions Networks
2.9. Statistics and Visualization
2.10. Special Case
3. Results
3.1. Overview of circRNA and lncRNA Expression Patterns in Cutaneous Melanoma Tissues
3.2. Differential Gene Expression of circRNAs and lncRNAs
3.3. Competitor Endogenous RNA Network (ceRNA Network)
3.4. IPA Functional Enrichment Analysis Based on the ceRNA Network
3.5. Prognostic Risk Score Using the Differentially Expressed ceRNA
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Oliver, J.; Onieva, J.L.; Garrido-Barros, M.; Berciano-Guerrero, M.-Á.; Sánchez-Muñoz, A.; José Lozano, M.; Farngren, A.; Álvarez, M.; Martínez-Gálvez, B.; Pérez-Ruiz, E.; et al. Association of Circular RNA and Long Non-Coding RNA Dysregulation with the Clinical Response to Immune Checkpoint Blockade in Cutaneous Metastatic Melanoma. Biomedicines 2022, 10, 2419. https://doi.org/10.3390/biomedicines10102419
Oliver J, Onieva JL, Garrido-Barros M, Berciano-Guerrero M-Á, Sánchez-Muñoz A, José Lozano M, Farngren A, Álvarez M, Martínez-Gálvez B, Pérez-Ruiz E, et al. Association of Circular RNA and Long Non-Coding RNA Dysregulation with the Clinical Response to Immune Checkpoint Blockade in Cutaneous Metastatic Melanoma. Biomedicines. 2022; 10(10):2419. https://doi.org/10.3390/biomedicines10102419
Chicago/Turabian StyleOliver, Javier, Juan Luis Onieva, Maria Garrido-Barros, Miguel-Ángel Berciano-Guerrero, Alfonso Sánchez-Muñoz, María José Lozano, Angela Farngren, Martina Álvarez, Beatriz Martínez-Gálvez, Elisabeth Pérez-Ruiz, and et al. 2022. "Association of Circular RNA and Long Non-Coding RNA Dysregulation with the Clinical Response to Immune Checkpoint Blockade in Cutaneous Metastatic Melanoma" Biomedicines 10, no. 10: 2419. https://doi.org/10.3390/biomedicines10102419