Next Article in Journal
Endothelial Dysfunction: From a Pathophysiological Mechanism to a Potential Therapeutic Target
Next Article in Special Issue
The Use of Drosophila to Understand Psychostimulant Responses
Previous Article in Journal
Sweet Taste Preference: Relationships with Other Tastes, Liking for Sugary Foods and Exploratory Genome-Wide Association Analysis in Subjects with Metabolic Syndrome
Previous Article in Special Issue
Oxytocin Attenuates the Stress-Induced Reinstatement of Alcohol-Seeking in Male Rats: Role of the Central Amygdala
Article

Alterations in the Proteome and Phosphoproteome Profiles of Rat Hippocampus after Six Months of Morphine Withdrawal: Comparison with the Forebrain Cortex

1
Laboratory of Membrane Receptors, Department of Neurochemistry, Institute of Physiology of the Czech Academy of Sciences, Videnska 1083, 142 20 Prague 4, Czech Republic
2
Laboratory of Translational Metabolism, Institute of Physiology of the Czech Academy of Sciences, Videnska 1083, 142 20 Prague 4, Czech Republic
3
Department of Physiology, Faculty of Science, Charles University, Vinicna 7, 128 43 Prague 2, Czech Republic
*
Author to whom correspondence should be addressed.
Academic Editor: Estela Castilla Ortega
Biomedicines 2022, 10(1), 80; https://doi.org/10.3390/biomedicines10010080
Received: 25 November 2021 / Revised: 21 December 2021 / Accepted: 28 December 2021 / Published: 31 December 2021
(This article belongs to the Special Issue Biological Aspects of Drug Addiction)
The knowledge about proteome changes proceeding during protracted opioid withdrawal is lacking. Therefore, the aim of this work was to analyze the spectrum of altered proteins in the rat hippocampus in comparison with the forebrain cortex after 6-month morphine withdrawal. We utilized 2D electrophoretic workflow (Pro-Q® Diamond staining and Colloidal Coomassie Blue staining) which was preceded by label-free quantification (MaxLFQ). The phosphoproteomic analysis revealed six significantly altered hippocampal (Calm1, Ywhaz, Tuba1b, Stip1, Pgk1, and Aldoa) and three cortical proteins (Tubb2a, Tuba1a, and Actb). The impact of 6-month morphine withdrawal on the changes in the proteomic profiles was higher in the hippocampus—14 proteins, only three proteins were detected in the forebrain cortex. Gene Ontology (GO) enrichment analysis of differentially expressed hippocampal proteins revealed the most enriched terms related to metabolic changes, cytoskeleton organization and response to oxidative stress. There is increasing evidence that energy metabolism plays an important role in opioid addiction. However, the way how morphine treatment and withdrawal alter energy metabolism is not fully understood. Our results indicate that the rat hippocampus is more susceptible to changes in proteome and phosphoproteome profiles induced by 6-month morphine withdrawal than is the forebrain cortex. View Full-Text
Keywords: protracted morphine withdrawal; rat hippocampus; rat brain cortex; gel-based proteomics; energy metabolism; oxidative stress; nLC-MS/MS protracted morphine withdrawal; rat hippocampus; rat brain cortex; gel-based proteomics; energy metabolism; oxidative stress; nLC-MS/MS
Show Figures

Figure 1

MDPI and ACS Style

Ujcikova, H.; Eckhardt, A.; Hejnova, L.; Novotny, J.; Svoboda, P. Alterations in the Proteome and Phosphoproteome Profiles of Rat Hippocampus after Six Months of Morphine Withdrawal: Comparison with the Forebrain Cortex. Biomedicines 2022, 10, 80. https://doi.org/10.3390/biomedicines10010080

AMA Style

Ujcikova H, Eckhardt A, Hejnova L, Novotny J, Svoboda P. Alterations in the Proteome and Phosphoproteome Profiles of Rat Hippocampus after Six Months of Morphine Withdrawal: Comparison with the Forebrain Cortex. Biomedicines. 2022; 10(1):80. https://doi.org/10.3390/biomedicines10010080

Chicago/Turabian Style

Ujcikova, Hana, Adam Eckhardt, Lucie Hejnova, Jiri Novotny, and Petr Svoboda. 2022. "Alterations in the Proteome and Phosphoproteome Profiles of Rat Hippocampus after Six Months of Morphine Withdrawal: Comparison with the Forebrain Cortex" Biomedicines 10, no. 1: 80. https://doi.org/10.3390/biomedicines10010080

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop