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Open AccessArticle

Screen-Printed Sensor Based on Potentiometric Transduction for Free Bilirubin Detection as a Biomarker for Hyperbilirubinemia Diagnosis

1
Department of Chemistry, Faculty of Science, Ain Shams University, Cairo 11566, Egypt
2
Pharmaceutical Chemistry Department, Drug Exploration & Development Chair (DEDC), College of Pharmacy, King Saud University, Riyadh 11451, Saudi Arabia
3
Applied Organic Chemistry Department, National Research Center, Dokki, Giza 12622, Egypt
4
Inorganic Chemistry Department, National Research Center, Dokki, Giza 12622, Egypt
*
Authors to whom correspondence should be addressed.
Chemosensors 2020, 8(3), 86; https://doi.org/10.3390/chemosensors8030086
Received: 16 July 2020 / Revised: 14 September 2020 / Accepted: 16 September 2020 / Published: 18 September 2020
(This article belongs to the Section Applied Chemical Sensors)
Novel reliable and cost-effective potentiometric screen-printed sensors for free bilirubin (BR) detection were presented. The sensors were fabricated using ordered mesoporous carbon (OMC) as an ion-to-electron transducer. The ion-association complex [Ni(bphen)3]2+[BR]2− was utilized as a sensory recognition material in the plasticized Polyvinyl Chloride (PVC) membrane. The membrane was drop-casted on the OMC layer, which is attached on a carbon conductor (2-mm diameter). In a 50 mM phosphate solution of pH 8.5, the electrodes offered a Nernstian slope of −26.8 ± 1.1 (r2 = 0.9997) mV/decade with a range of linearity 1.0 × 10−6–1 × 10−2 M towards free bilirubin with a detection limit 8.8 × 10−7 M (0.52 µg/mL). The presented sensors offered good features in terms of reliability, ease of design, high potential stability, high specificity and good accuracy and precision. Chronopotentiometric and electrochemical impedance spectrometric measurements were used for short-term potential stability and interfacial capacitance calculations. The sensors were used for the determination of free bilirubin in biological fluids. The data obtained are fairly well consistent with those obtained by the reference spectophotometric method. Based on the interaction of free BR with albumin (1:1), the sensors were also utilized for the assessment of albumin in human serum. View Full-Text
Keywords: screen-printed; ordered mesoporous carbon (OMC); free bilirubin; human serum albumin (HSA); point-of-care diagnostics screen-printed; ordered mesoporous carbon (OMC); free bilirubin; human serum albumin (HSA); point-of-care diagnostics
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MDPI and ACS Style

H. Kamel, A.; Amr, A. .-G.; Galal, H.R.; Al-Omar, M.A.; Almehizia, A.A. Screen-Printed Sensor Based on Potentiometric Transduction for Free Bilirubin Detection as a Biomarker for Hyperbilirubinemia Diagnosis. Chemosensors 2020, 8, 86.

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