Next Article in Journal / Special Issue
Development and Application of Electrochemical Sensor Based on Molecularly Imprinted Polymer and Carbon Nanotubes for the Determination of Carvedilol
Previous Article in Journal
Recent Trends in Field-Effect Transistors-Based Immunosensors
Open AccessArticle

Molecularly Imprinted Quartz Crystal Microbalance Sensor (QCM) for Bilirubin Detection

1
Department of Chemistry, Hacettepe University, 06800 Ankara, Turkey
2
Department of Chemical Technology, Abant İzzet Baysal University, 14900 Bolu, Turkey
*
Author to whom correspondence should be addressed.
Academic Editor: Peter Lieberzeit
Chemosensors 2016, 4(4), 21; https://doi.org/10.3390/chemosensors4040021
Received: 22 August 2016 / Revised: 24 October 2016 / Accepted: 31 October 2016 / Published: 18 November 2016
This study aims the preparation of a QCM sensor for the detection of bilirubin in human plasma. Bilirubin-imprinted poly-(2-hydroxyethyl methacrylate-N-methacryloyl-l-tryptophan methyl ester) (PHEMATrp) nanofilm (MIP) on the gold surface of a QCM chip was synthesized by the molecular imprinting technique. Meanwhile, the non-imprinted PHEMATrp (NIP) nanofilm was synthesized by the same experimental technique to examine the imprinting effect. Characterization of MIP and NIP nanofilms on the QCM chip surface was achieved by atomic force microscopy (AFM), ellipsometry, Fourier transform infrared spectrophotometry-attenuated total reflectance (FTIR-ATR) and contact angle measurements (CA). The observations indicated that the nanofilm was almost in a monolayer. Thereinafter, the imprinted and the non-imprinted QCM chips were connected to the QCM system to investigate kinetic and affinity properties. In order to examine the selectivity of the MIP-PHEMATrp nanofilm, competitive adsorption of bilirubin with cholesterol and estradiol was performed. Limit of detection (LOD) and limit of quantitation (LOQ) values were calculated as 0.45 μg/mL and 0.9 μg/mL, respectively. View Full-Text
Keywords: molecular imprinting technique; bilirubin; QCM; PHEMA; nanosensor molecular imprinting technique; bilirubin; QCM; PHEMA; nanosensor
Show Figures

Graphical abstract

MDPI and ACS Style

Çiçek, Ç.; Yılmaz, F.; Özgür, E.; Yavuz, H.; Denizli, A. Molecularly Imprinted Quartz Crystal Microbalance Sensor (QCM) for Bilirubin Detection. Chemosensors 2016, 4, 21.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop