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Proteomes 2018, 6(2), 14; https://doi.org/10.3390/proteomes6020014

The Secretome and N-Glycosylation Profiles of the Charophycean Green Alga, Penium margaritaceum, Resemble Those of Embryophytes

1
Plant Biology Section, School of Integrative Plant Science, Cornell University, Ithaca, NY 14853, USA
2
Red de Estudios Moleculares Avanzados, Instituto de Ecología A. C., Cluster BioMimic, Carretera Antigua a Coatepec 351, Congregación el Haya, CP 91070 Xalapa, Veracruz, Mexico
3
Boyce Thompson Institute, Ithaca, NY 14853, USA
4
U.S. Department of Agriculture-Agricultural Research Service, Robert W. Holley Center for Agriculture and Health, Ithaca, NY 14853, USA
5
Institute of Biotechnology, Cornell University, Ithaca, NY 14853, USA
6
Department of Biology and Skidmore Microscopy Imaging Center, Skidmore College, Saratoga Springs, NY 12866, USA
*
Author to whom correspondence should be addressed.
Received: 10 January 2018 / Revised: 13 March 2018 / Accepted: 14 March 2018 / Published: 21 March 2018
(This article belongs to the Special Issue Plant Proteomics 2017)
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Abstract

The secretome can be defined as the population of proteins that are secreted into the extracellular environment. Many proteins that are secreted by eukaryotes are N-glycosylated. However, there are striking differences in the diversity and conservation of N-glycosylation patterns between taxa. For example, the secretome and N-glycosylation structures differ between land plants and chlorophyte green algae, but it is not clear when this divergence took place during plant evolution. A potentially valuable system to study this issue is provided by the charophycean green algae (CGA), which is the immediate ancestors of land plants. In this study, we used lectin affinity chromatography (LAC) coupled with mass spectrometry to characterize the secretome including secreted N-glycoproteins of Penium margaritaceum, which is a member of the CGA. The identified secreted proteins and N-glycans were compared to those known from the chlorophyte green alga Chlamydomonas reinhardtii and the model land plant, Arabidopsis thaliana, to establish their evolutionary context. Our approach allowed the identification of cell wall proteins and proteins modified with N-glycans that are identical to those of embryophytes, which suggests that the P. margaritaceum secretome is more closely related to those of land plants than to those of chlorophytes. The results of this study support the hypothesis that many of the proteins associated with plant cell wall modification as well as other extracellular processes evolved prior to the colonization of terrestrial habitats. View Full-Text
Keywords: secretome; cell wall proteins; embryophytes; charophycean green algae; N-glycosylation secretome; cell wall proteins; embryophytes; charophycean green algae; N-glycosylation
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Ruiz-May, E.; Sørensen, I.; Fei, Z.; Zhang, S.; Domozych, D.S.; Rose, J.K.C. The Secretome and N-Glycosylation Profiles of the Charophycean Green Alga, Penium margaritaceum, Resemble Those of Embryophytes. Proteomes 2018, 6, 14.

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