Arabidopsis Regenerating Protoplast: A Powerful Model System for Combining the Proteomics of Cell Wall Proteins and the Visualization of Cell Wall Dynamics
Abstract
:1. Introduction
2. Preparation of Protoplasts and Cell Wall Regeneration from Protoplasts
3. Visualization of Cell Wall Dynamics in Regenerating Protoplast
4. Preparation of Cell Wall Proteins
4.1. Conventional Extraction Procedure for Cell Wall Proteins
4.2. Extraction Procedure for Cell Wall Proteins from Protoplast
4.3. Problem of the Extraction Procedure for Cell Wall Proteins
4.4. Cell Wall Protein Purification Techniques
4.5. Improved Extraction Procedure for Cell Wall Protein from Protoplasts
5. Protein Separation and Mass Spectrometry for Identification
6. Xyloglucan Metabolism in Regenerating Protoplasts
7. Concluding Remarks
Supplementary Materials
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Family * | AGI | Description ** | Protein Name | 1 h | 2 h | Native | References |
---|---|---|---|---|---|---|---|
GH1 | At1g66280 | β-Glucosidase | Bglu22 | + | + | ||
GH1 | At2g44450 | β-Glucosidase | Bglu15 | + | |||
GH1 | At3g09260 | β-Glucosidase | Bglu23/PYK10 | + | Ogasawara et al., 2009 [68] | ||
GH3 | At5g20950 | β-Xylosidase | + | + | + | ||
GH9 | At1g71380 | β-1,4-Glucanase | CEL3 | + | Lewis et al., 2013 [69] | ||
GH16 | At3g48580 | Xyloglucan | XTH11 | + | + | + | Yokoyama et al., 2010 [70] |
endotransglucosylase/hydrolase | |||||||
GH18 | At5g24090 | Chitinase | CHIA | + | Takenaka et al., 2009 [71] | ||
GH19 | At2g43610 | Chitinase | + | ||||
GH27 | At5g08380 | α-Galactosidase | AGAL1 | + | + | + | |
GH28 | At5g41870 | Polygalacturonase | + | Cao, 2012 [72] | |||
GH28 | At5g06860 | Polygalacturonase | PGIP1 | + | Ferrari et al., 2012 [73] | ||
GH31 | At1g68560 | α-Xylosidase | AXY3/XYL1 | + | + | + | Günl and Pauly, 2011 [74] |
GH32 | At3g13790 | β-Fructosidase | CWI | + | + | + | Mazola et al., 2015 [75] |
GH35 | At5g63810 | β-Galactosidase | BGAL10 | + | + | + | Sampedro et al., 2012 [76] |
GH38 | At3g26720 | α-Mannosidase | + | + | + | ||
EXP | At3g45970 | Expansin | EXPL1 | + |
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Yokoyama, R.; Kuki, H.; Kuroha, T.; Nishitani, K. Arabidopsis Regenerating Protoplast: A Powerful Model System for Combining the Proteomics of Cell Wall Proteins and the Visualization of Cell Wall Dynamics. Proteomes 2016, 4, 34. https://doi.org/10.3390/proteomes4040034
Yokoyama R, Kuki H, Kuroha T, Nishitani K. Arabidopsis Regenerating Protoplast: A Powerful Model System for Combining the Proteomics of Cell Wall Proteins and the Visualization of Cell Wall Dynamics. Proteomes. 2016; 4(4):34. https://doi.org/10.3390/proteomes4040034
Chicago/Turabian StyleYokoyama, Ryusuke, Hiroaki Kuki, Takeshi Kuroha, and Kazuhiko Nishitani. 2016. "Arabidopsis Regenerating Protoplast: A Powerful Model System for Combining the Proteomics of Cell Wall Proteins and the Visualization of Cell Wall Dynamics" Proteomes 4, no. 4: 34. https://doi.org/10.3390/proteomes4040034