AtPPRT1, an E3 Ubiquitin Ligase, Enhances the Thermotolerance in Arabidopsis
Abstract
:1. Introduction
2. Materials and Methods
2.1. Plant Materials and Growth Conditions
2.2. Quantitative Real-Time-PCR (qRT-PCR) Analysis
2.3. GUS Histochemical Staining
2.4. Phenotypic Analysis
2.5. DAB and H2DCFDA Staining
2.6. Measurement of H2O2 and MDA
3. Results
3.1. Expression Pattern Analysis of AtPPRT1 under Heat Stress
3.2. AtPPRT1 Enhances Thermotolerance in Arabidopsis
3.3. AtPPRT1 Reduced the Accumulation of ROS Caused by Heat Stress in Arabidopsis
3.4. Altered Expression of AtPPRT1 Leads to Altered Induction of Heat-Related Genes
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
References
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Liu, Y.; Xiao, S.; Sun, H.; Pei, L.; Liu, Y.; Peng, L.; Gao, X.; Liu, Y.; Wang, J. AtPPRT1, an E3 Ubiquitin Ligase, Enhances the Thermotolerance in Arabidopsis. Plants 2020, 9, 1074. https://doi.org/10.3390/plants9091074
Liu Y, Xiao S, Sun H, Pei L, Liu Y, Peng L, Gao X, Liu Y, Wang J. AtPPRT1, an E3 Ubiquitin Ligase, Enhances the Thermotolerance in Arabidopsis. Plants. 2020; 9(9):1074. https://doi.org/10.3390/plants9091074
Chicago/Turabian StyleLiu, Yu, Shuya Xiao, Haoran Sun, Linsen Pei, Yingying Liu, Lu Peng, Xuemeng Gao, Yu Liu, and Jianmei Wang. 2020. "AtPPRT1, an E3 Ubiquitin Ligase, Enhances the Thermotolerance in Arabidopsis" Plants 9, no. 9: 1074. https://doi.org/10.3390/plants9091074