Analysis of the Antimicrobial, Cytotoxic, and Antioxidant Activities of Cnidium officinale Extracts
Abstract
:1. Introduction
2. Results
2.1. Inhibition Zone Test
2.2. Colony-Forming Unit (CFU) Counts
2.3. Optical Density (OD)
2.4. Scanning Electron Microscopy (SEM)
2.5. Biological Properties
2.6. Measurement of Polyphenol and Flavonoid Contents
3. Discussion
4. Materials and Methods
4.1. Materials
4.2. Microbial Preparation
4.3. Inhibition Zone Test
4.4. OD
4.5. CFUs
4.6. SEM
4.7. Biological Properties
4.8. Measurement of Polyphenol and Flavonoid Contents
4.9. Statistical Analyses
5. Conclusions
- In the inhibition zone test, the growth inhibition zone was observed to increase; the inhibition zones of both strains of microbes showed significant differences as compared to that of the control (p < 0.05) group.
- CFU measurement showed a reduction in the number of colonies; both the strains of microbes demonstrated a significant difference in CFU reduction as compared to that of the control (p < 0.05) group.
- The OD values decreased, such that both the strains of microbes showed a significant difference in OD as compared to that of the control group after 12 and 24 h (p < 0.05).
- SEM analysis demonstrated a tendency for both the microbial numbers to decrease as compared to that of the control group.
- In cell viability studies, no significant differences were observed in the control group and the 50 μg/mL solution (p > 0.05); significant differences in cell viability were observed in 100, 150, and 200 μg/mL groups as compared to that of the control (p < 0.05) group.
- In the analysis of polyphenol and flavonoid contents, significant differences were observed between the control and experimental groups (p < 0.05).
Author Contributions
Funding
Conflicts of Interest
References
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Lee, M.-J.; Kang, M.-K. Analysis of the Antimicrobial, Cytotoxic, and Antioxidant Activities of Cnidium officinale Extracts. Plants 2020, 9, 988. https://doi.org/10.3390/plants9080988
Lee M-J, Kang M-K. Analysis of the Antimicrobial, Cytotoxic, and Antioxidant Activities of Cnidium officinale Extracts. Plants. 2020; 9(8):988. https://doi.org/10.3390/plants9080988
Chicago/Turabian StyleLee, Myung-Jin, and Min-Kyung Kang. 2020. "Analysis of the Antimicrobial, Cytotoxic, and Antioxidant Activities of Cnidium officinale Extracts" Plants 9, no. 8: 988. https://doi.org/10.3390/plants9080988