Jaceidin Flavonoid Isolated from Chiliadenus montanus Attenuates Tumor Progression in Mice via VEGF Inhibition: In Vivo and In Silico Studies
Abstract
:1. Introduction
2. Results and Discussion
2.1. Identification of the Isolated Compounds
2.2. Screening for Antitumor Activity
2.2.1. In Vitro Assay
2.2.2. In Vivo Assay
Tumor Weight
Histopathological Examination of Solid Tumors
2.2.3. Enzyme-Linked Immunosorbent Assays
Determination of GSH and MDA Levels
Estimation of VEGF-B Levels
Molecular Modeling and Drug Design
In Silico ADME Predictive Study
3. Material and Methods
3.1. Instruments
3.2. Collection of Plant Materials
3.3. Extraction and Isolation
3.4. Screening for Antitumor Activity
3.4.1. In Vitro Assay
3.4.2. In Vivo Assay
Induction of EAC Solid Tumors in Mice
Experimental Design
Histopathological Examination of Solid Tumors
3.4.3. Enzyme-Linked Immunosorbent Assays
3.4.4. Molecular Modeling and Drug Design
3.4.5. In Silico ADME Predictive Study
3.4.6. Statistical Analysis
4. Conclusions
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Compound No. | HepG2 (IC50) a,b in µM | MCF-7 (IC50) a,b in µM | HFB-4 (IC50) a,b in µM |
---|---|---|---|
1 | 48.2 ± 0.30 | 45.6 ± 0.50 | >200 |
2 | 39.7 ± 0.65 | 41.5 ± 0.55 | >200 |
3 | 30.5 ± 0.61 | 10.7 ± 0.40 | >200 |
4 | 9.7 ± 0.26 | 9.3 ± 0.30 | >200 |
5 | 33.4 ± 0.49 | 38.5 ± 0.55 | >200 |
6 | 35.1 ± 0.60 | 33.2 ± 0.35 | >200 |
Doxorubicin c | 4.58 ± 0.47 | 4 ± 0.25 | 14.7 ± 0.50 |
ADME Prediction Parameters | Compound | Reference Flavonoid | ||
---|---|---|---|---|
3 | 4 | Quercetin | Genistein | |
Mol-MW a | 344.3 | 360.3 | 302.2 | 286.2 |
DonorHB b | 1 | 2 | 3 | 4 |
AcceptHB c | 5.2 | 6 | 5.2 | 4.5 |
QPlogPo/w d | 2.7 | 2.2 | 0.517 | 1.05 |
#rotor e | 5 | 6 | 5 | 4 |
PSA f | 95.3 | 115.5 | 140.08 | 118.6 |
QPlogS g | −4.1 | −3.8 | −3.09 | −3.05 |
QPlogHERG h | −4.9 | −4.7 | −5.3 | −5.07 |
QPPCaco i | 549.5 | 287.7 | 21.03 | 58.2 |
QPP MDCK j | 259.01 | 128.7 | 7.62 | 22.89 |
QPlogKhsa k | 0.163 | 0.013 | −0.318 | −0.198 |
% Human Oral Absorption l | 92.2 | 83.92 | 53.65 | 64.7 |
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Elhady, S.S.; Eltamany, E.E.; Shaaban, A.E.; Bagalagel, A.A.; Muhammad, Y.A.; El-Sayed, N.M.; Ayyad, S.N.; Ahmed, A.A.M.; Elgawish, M.S.; Ahmed, S.A. Jaceidin Flavonoid Isolated from Chiliadenus montanus Attenuates Tumor Progression in Mice via VEGF Inhibition: In Vivo and In Silico Studies. Plants 2020, 9, 1031. https://doi.org/10.3390/plants9081031
Elhady SS, Eltamany EE, Shaaban AE, Bagalagel AA, Muhammad YA, El-Sayed NM, Ayyad SN, Ahmed AAM, Elgawish MS, Ahmed SA. Jaceidin Flavonoid Isolated from Chiliadenus montanus Attenuates Tumor Progression in Mice via VEGF Inhibition: In Vivo and In Silico Studies. Plants. 2020; 9(8):1031. https://doi.org/10.3390/plants9081031
Chicago/Turabian StyleElhady, Sameh S., Enas E. Eltamany, Amera E. Shaaban, Alaa A. Bagalagel, Yosra A. Muhammad, Norhan M. El-Sayed, Seif N. Ayyad, Amal A. M. Ahmed, Mohamed S. Elgawish, and Safwat A. Ahmed. 2020. "Jaceidin Flavonoid Isolated from Chiliadenus montanus Attenuates Tumor Progression in Mice via VEGF Inhibition: In Vivo and In Silico Studies" Plants 9, no. 8: 1031. https://doi.org/10.3390/plants9081031