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Open AccessArticle

Characterization of Atypical Protein Tyrosine Kinase (PTK) Genes and Their Role in Abiotic Stress Response in Rice

1
Division of Plant Physiology, ICAR-Indian Agricultural Research Institute, New Delhi 110012, India
2
ICAR-National Institute for Plant Biotechnology, New Delhi 110012, India
3
Division of Biochemistry, ICAR-Indian Agricultural Research Institute, New Delhi 110012, India
*
Authors to whom correspondence should be addressed.
Plants 2020, 9(5), 664; https://doi.org/10.3390/plants9050664
Received: 4 April 2020 / Revised: 14 May 2020 / Accepted: 14 May 2020 / Published: 23 May 2020
(This article belongs to the Section Plant Genetics and Genomics)
Tyrosine phosphorylation constitutes up to 5% of the total phophoproteome. However, only limited studies are available on protein tyrosine kinases (PTKs) that catalyze protein tyrosine phosphorylation in plants. In this study, domain analysis of the 27 annotated PTK genes in rice genome led to the identification of 18 PTKs with tyrosine kinase domain. The kinase domain of rice PTKs shared high homology with that of dual specificity kinase BRASSINOSTEROID-INSENSITIVE 1 (BRI1) of Arabidopsis. In phylogenetic analysis, rice PTKs clustered with receptor-like cytoplasmic kinases-VII (RLCKs-VII) of Arabidopsis. mRNAseq analysis using Genevestigator revealed that rice PTKs except PTK9 and PTK16 express at moderate to high level in most tissues. PTK16 expression was highly abundant in panicle at flowering stage. mRNAseq data analysis led to the identification of drought, heat, salt, and submergence stress regulated PTK genes in rice. PTK14 was upregulated under all stresses. qRT-PCR analysis also showed that all PTKs except PTK10 were significantly upregulated in root under osmotic stress. Tissue specificity and abiotic stress mediated differential regulation of PTKs suggest their potential role in development and stress response of rice. The candidate dual specificity PTKs identified in this study paves way for molecular analysis of tyrosine phosphorylation in rice. View Full-Text
Keywords: BRI1; drought; dual specificity; osmotic stress; receptor-like protein kinases BRI1; drought; dual specificity; osmotic stress; receptor-like protein kinases
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Elangovan, A.; Dalal, M.; Krishna, G.K.; Devika, S.; Kumar, R.R.; Sathee, L.; Chinnusamy, V. Characterization of Atypical Protein Tyrosine Kinase (PTK) Genes and Their Role in Abiotic Stress Response in Rice. Plants 2020, 9, 664.

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