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Article

Effects of NtSPS1 Overexpression on Solanesol Content, Plant Growth, Photosynthesis, and Metabolome of Nicotiana tabacum

1
Tobacco Research Institute of Chinese Academy of Agricultural Sciences, Qingdao 266101, China
2
Yunnan Tobacco Leaf Company, Kunming 650000, China
3
Anhui Wannan Tobacco Leaf Co., Ltd., Xuancheng 242000, China
4
Guelph Food Research Center, Agriculture and Agri-Food Canada, Guelph, ON N1G 5C9, Canada
*
Authors to whom correspondence should be addressed.
Plants 2020, 9(4), 518; https://doi.org/10.3390/plants9040518
Received: 22 February 2020 / Revised: 12 April 2020 / Accepted: 16 April 2020 / Published: 17 April 2020
(This article belongs to the Special Issue Biosynthesis and Functions of Terpenoids in Plants)
Nicotiana tabacum solanesyl diphosphate synthase 1 (NtSPS1) is the key enzyme in solanesol biosynthesis. However, changes in the solanesol content, plant growth, photosynthesis, and metabolome of tobacco plants after NtSPS1 overexpression (OE) have not been previously reported. In the present study, these parameters, as well as photosynthetic gas exchange, chlorophyll content, and chlorophyll fluorescence parameters, were compared between NtSPS1 OE and wild type (WT) lines of tobacco. As expected, NtSPS1 OE significantly increased solanesol content in tobacco leaves. Although NtSPS1 OE significantly increased leaf growth, photosynthesis, and chlorophyll content, the chlorophyll fluorescence parameters in the leaves of the NtSPS1 OE lines were only slightly higher than those in the WT leaves. Furthermore, NtSPS1 OE resulted in 64 differential metabolites, including 30 up-regulated and 34 down-regulated metabolites, between the OE and WT leaves. Pathway enrichment analysis of these differential metabolites identified differentially enriched pathways between the OE and WT leaves, e.g., carbon fixation in photosynthetic organisms. The maximum carboxylation rate of RuBisCO and the maximum rate of RuBP regeneration were also elevated in the NtSPS1 OE line. To our knowledge, this is the first study to confirm the role of NtSPS1 in solanesol biosynthesis and its possible functional mechanisms in tobacco. View Full-Text
Keywords: Nicotiana tabacum; solanesol; solanesyl diphosphate synthase; plant growth; photosynthesis; metabolome Nicotiana tabacum; solanesol; solanesyl diphosphate synthase; plant growth; photosynthesis; metabolome
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MDPI and ACS Style

Yan, N.; Gai, X.; Xue, L.; Du, Y.; Shi, J.; Liu, Y. Effects of NtSPS1 Overexpression on Solanesol Content, Plant Growth, Photosynthesis, and Metabolome of Nicotiana tabacum. Plants 2020, 9, 518. https://doi.org/10.3390/plants9040518

AMA Style

Yan N, Gai X, Xue L, Du Y, Shi J, Liu Y. Effects of NtSPS1 Overexpression on Solanesol Content, Plant Growth, Photosynthesis, and Metabolome of Nicotiana tabacum. Plants. 2020; 9(4):518. https://doi.org/10.3390/plants9040518

Chicago/Turabian Style

Yan, Ning; Gai, Xiaolei; Xue, Lin; Du, Yongmei; Shi, John; Liu, Yanhua. 2020. "Effects of NtSPS1 Overexpression on Solanesol Content, Plant Growth, Photosynthesis, and Metabolome of Nicotiana tabacum" Plants 9, no. 4: 518. https://doi.org/10.3390/plants9040518

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