Two Rye Genes Responsible for Abnormal Development of Wheat–Rye Hybrids Are Linked in the Vicinity of an Evolutionary Translocation on Chromosome 6R
Abstract
:1. Introduction
2. Results
2.1. The Linkage of Mutant Genes with Molecular Markers
2.2. Comparative Mapping
2.3. Joint Segregation Analysis of Hybrid Dwarfness and Embryo Lethality
3. Discussion
4. Materials and Methods
4.1. Plant Material
4.2. Phenotyping and Genotyping
4.3. Linkage Map Construction and Comparative Mapping
Author Contributions
Funding
Conflicts of Interest
References
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Marker a | BLASTN Query a | Rye | Barley | ||||||
---|---|---|---|---|---|---|---|---|---|
Subject | Expect | Chr b | pos | Subject | s_start | s_end | Expect | ||
GRM0173 | Sce_Assembly02_c6346 | Lo7_v2_contig_257767 | 1 × 10−100 | 0R | chr6H | 570137757 | 570137532 | 6 × 10−59 | |
GRM0959 | Sce_Assembly02_c87163 | Lo7_v2_contig_4801 | 3 × 10−145 | 6R | 108.7 | chr6H | 579212782 | 579213063 | 9 × 10−43 |
GRM0902 | Sce_Assembly02_c81266 | Lo7_v2_contig_1427427 | 1 × 10−104 | 0R | chr3H | 19821332 | 19821201 | 2 × 10−12 | |
GRM0130 | Sce_Assembly02_c4514 | Lo7_v2_contig_126444 | 5 × 10−167 | 6R | 124.6 | chr3H | 674285409 | 674285721 | 5 × 10−122 |
Hybrid Combination | Seeds with Normal Embryo (Eml-R1a) | Seeds with Abnormal Embryo (Eml-R1b) | χ2 1 Eml-R1a: 1 Eml-R1b | |||
---|---|---|---|---|---|---|
Alive | Dead | Undifferentiated Embryo | Without Embryo | |||
Dwarf | Normal | |||||
CS × F1(V1 × L2) | 215 | 15 | 34 (6.6) * | 244 | 10 | 1.19 |
CS × F1(L2 × V1) | 121 | 4 | 51 (15.5) * | 145 | 7 | 1.76 |
CS × F1(L2 × V10) | 111 | 11 | 228 (65.9) * | 321 | 37 | 0.09 |
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Tsvetkova, N.V.; Tikhenko, N.D.; Hackauf, B.; Voylokov, A.V. Two Rye Genes Responsible for Abnormal Development of Wheat–Rye Hybrids Are Linked in the Vicinity of an Evolutionary Translocation on Chromosome 6R. Plants 2018, 7, 55. https://doi.org/10.3390/plants7030055
Tsvetkova NV, Tikhenko ND, Hackauf B, Voylokov AV. Two Rye Genes Responsible for Abnormal Development of Wheat–Rye Hybrids Are Linked in the Vicinity of an Evolutionary Translocation on Chromosome 6R. Plants. 2018; 7(3):55. https://doi.org/10.3390/plants7030055
Chicago/Turabian StyleTsvetkova, Natalia V., Natalia D. Tikhenko, Bernd Hackauf, and Anatoly V. Voylokov. 2018. "Two Rye Genes Responsible for Abnormal Development of Wheat–Rye Hybrids Are Linked in the Vicinity of an Evolutionary Translocation on Chromosome 6R" Plants 7, no. 3: 55. https://doi.org/10.3390/plants7030055