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Perturbation Analysis of Calcium, Alkalinity and Secretion during Growth of Lily Pollen Tubes
Open AccessArticle

Pollen Grain Preservation and Fertility in Valuable Commercial Rose Cultivars

Council for Agricultural Research and Economics (CREA), Ornamental Species Research Unit, Corso degli Inglesi 508, 18038 Sanremo (IM), Italy
Department of Biology and Biotechnology “Lazzaro Spallanzani” (DBB), via Ferrata 9, 27100 Pavia, Italy
Department of Agricultural, Forest and Food Sciences, University of Turin, Largo Paolo Braccini 2, 10095 Grugliasco (TO), Italy
NIRP International, Az. Agricola di Ghione L. & Figli, via San Rocco 1, Fraz. Bevera, 18039 Ventimiglia (IM), Italy
Author to whom correspondence should be addressed.
Academic Editor: Giampiero Cai
Plants 2017, 6(2), 17;
Received: 2 March 2017 / Revised: 13 April 2017 / Accepted: 14 April 2017 / Published: 24 April 2017
(This article belongs to the Special Issue Pollen Tube Growth 2016)
In the cut flower market, traditional breeding is still the best way to achieve new rose cultivars. The geographical delocalization of cultivar constitution (generally made in Europe and North America) and plant cultivation (large areas in Africa and South America) represents a limit point for crossing and selection. Rose breeders often need to overcome geographical distances, resulting in asynchrony in flowering among crossing parents, by storing and sending pollen. Hence, a key aspect in breeding programs is linked to pollen availability and conservation, jointly with the identification of parameters related to pollen fertility. In this study we present the results of three different trials. In the first, pollen diameter and pollen viability were chosen as fertility predictors of 10 Rosa hybrida commercial cultivars. In the second trial, aliquots of dried pollen grains of six R. hybrida cultivar were stored under two different temperatures (freezer at T = −20 °C and deep freezer at T = −80 °C) and after a wide range of conservation period, their viability was measured. In the third trial, the effective fertilization capacity of frozen pollen of 19 pollen donor cultivars was evaluated during 2015 crossing breeding plan, performing 44 hybridizations and correlating the number of seeds and the ratio seeds/crossing, obtained by each cultivar, with in vitro pollen germination ability. View Full-Text
Keywords: anther conservation; frozen pollen; in vitro germination; pollen tube growth anther conservation; frozen pollen; in vitro germination; pollen tube growth
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MDPI and ACS Style

Giovannini, A.; Macovei, A.; Caser, M.; Mansuino, A.; Ghione, G.G.; Savona, M.; Carbonera, D.; Scariot, V.; Balestrazzi, A. Pollen Grain Preservation and Fertility in Valuable Commercial Rose Cultivars. Plants 2017, 6, 17.

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