Multiple Autoregulation of Nodulation (AON) Signals Identified through Split Root Analysis of Medicago truncatula sunn and rdn1 Mutants
Abstract
:1. Introduction
2. Results and Discussion
2.1. AON Mutants Lack an Early Systemic Response to Previous Nodulation Events, but Suppression Returns after 10–15 Days
2.2. AON Mutants and Wild Type Fix Equivalent Amounts of Nitrogen at Time Points when Systemic Suppression Is Observed
2.3. Nitrate Reduces Nodulation in Both AON Mutants and Wild Type Plants in this System
2.4. Inoculation with Fix− or Nod+/Fix− Rhizobia Did Not Elicit the AON Response in all Plants
2.5. Nutrient Stress Causes Suppression of Nodulation in Wild Type and AON Mutants
3. Experimental Section
3.1. Plant Materials and Growth Conditions
3.2. Split Root Development and Inoculation
3.3. Rhizobial Strains and Growth Conditions
3.4. Nitrogen Fixation Measurements
3.5. Determination of C and N
3.6. Statistical Analysis
4. Conclusions
Acknowledgments
Author Contributions
Conflicts of Interest
References and Notes
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Kassaw, T.; Jr., W.B.; Frugoli, J. Multiple Autoregulation of Nodulation (AON) Signals Identified through Split Root Analysis of Medicago truncatula sunn and rdn1 Mutants. Plants 2015, 4, 209-224. https://doi.org/10.3390/plants4020209
Kassaw T, Jr. WB, Frugoli J. Multiple Autoregulation of Nodulation (AON) Signals Identified through Split Root Analysis of Medicago truncatula sunn and rdn1 Mutants. Plants. 2015; 4(2):209-224. https://doi.org/10.3390/plants4020209
Chicago/Turabian StyleKassaw, Tessema, William Bridges Jr., and Julia Frugoli. 2015. "Multiple Autoregulation of Nodulation (AON) Signals Identified through Split Root Analysis of Medicago truncatula sunn and rdn1 Mutants" Plants 4, no. 2: 209-224. https://doi.org/10.3390/plants4020209