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Article

Characterizing the Genetic Basis of Winter Wheat Rust Resistance in Southern Kazakhstan

by
Shynbolat Rsaliyev
1,*,
Elena Gultyaeva
2,
Olga Baranova
2,
Alma Kokhmetova
3,
Rahim Urazaliev
1,
Ekaterina Shaydayuk
2,
Akbope Abdikadyrova
1 and
Galiya Abugali
1,4
1
Kazakh Research Institute of Agriculture and Plant Growing, Almalybak 040909, Kazakhstan
2
All Russian Institute of Plant Protection, Shosse Podbelskogo 3, 196608 St. Petersburg, Russia
3
Institute of Plant Biology and Biotechnology, Almaty 050040, Kazakhstan
4
Faculty of Agrobiology, Kazakh National Agrarian Research University, Almaty 050010, Kazakhstan
*
Author to whom correspondence should be addressed.
Plants 2025, 14(7), 1146; https://doi.org/10.3390/plants14071146
Submission received: 7 March 2025 / Revised: 27 March 2025 / Accepted: 31 March 2025 / Published: 7 April 2025
Versions Notes

Abstract

:
In an effort to enhance wheat’s resilience against rust diseases, our research explores the genetic underpinnings of resistance in a diverse collection of winter bread wheat accessions. Leaf rust (Puccinia triticina), yellow rust (Puccinia striiformis f. sp. tritici), and stem rust (Puccinia graminis f. sp. tritici) are significant threats to global wheat production. By leveraging host genetic resistance, we can improve disease management strategies. Our study evaluated 55 wheat accessions, including germplasm from Kazakhstan, from Uzbekistan, from Russia, from Kyrgyzstan, France, and CIMMYT under field conditions in southern Kazakhstan from 2022 to 2024. The results showed a robust resistance profile: 49.1% of accessions exhibited high to moderate resistance to leaf rust, 12.7% to yellow rust, and 30.9% to stem rust. Notably, ten accessions demonstrated resistance to multiple rust species, while seven showed resistance to two rusts. Twenty accessions were selected for further seedling resistance and molecular analysis. Three accessions proved resistant to six isolates of P. triticina, two to four isolates of P. striiformis, and four to five isolates of P. graminis. Although no genotypes were found to be universally resistant to all rust species at the seedling stage, two accessions—Bezostaya 100 (Russia) and KIZ 90 (Kazakhstan)—displayed consistent resistance to leaf and stem rust in both seedling and field evaluations. Molecular analysis revealed the presence of key resistance genes, including Lr1, Lr3, Lr26, Lr34, Yr9, Yr18, Sr31, Sr57, and the 1AL.1RS translocation. This work provides valuable insights into the genetic landscape of wheat rust resistance and contributes to the development of new wheat cultivars that can withstand these diseases, enhancing global food security.

1. Introduction

Winter bread wheat (Triticum aestivum L.) is a crucial agricultural crop in Kazakhstan, widely cultivated in the southern and southeastern regions, including Almaty, Zhetysu, Zhambyl, and Turkestan, on both rain-fed and irrigated lands. Over the past decade, these regions have experienced significant climate changes, characterized by warmer winters and warmer, more humid springs with a slight increase in annual precipitation [1,2]. These shifts in weather patterns are marked by fluctuations both annually and monthly [3,4], which can exacerbate disease development in crops. Among the significant biotic factors affecting wheat production are rust pathogens. Leaf rust, caused by Puccinia triticina, yellow (stripe) rust by P. striiformis f. sp. tritici, and stem rust by P. graminis f. sp. tritici, are major threats to wheat yields. Understanding and addressing these challenges is crucial for maintaining the health and productivity of wheat crops in Kazakhstan.
In Kazakhstan, leaf rust is a prevalent issue affecting wheat crops [5,6,7,8], with epidemics occurring approximately every 4 years. This frequency is linked to an expansion in wheat cultivation [9]. The leaf rust population in Kazakhstan is genetically diverse, as evidenced by the wide range of virulence among studied races. Recent studies have identified 25 different races of wheat leaf rust in the country. An analysis using 16 Lr lines revealed diverse virulence types, ranging from less virulent strains like “CJF/B” and “JCL/G” to highly virulent ones such as “TKT/Q”. Most pathotypes were avirulent to Lr9, Lr19, Lr24, and Lr25 but virulent to Lr1, Lr2a, Lr3ka, Lr11, and Lr30 [7]. Kazakhstani wheat cultivars and lines primarily contain leaf rust-resistance genes such as Lr1, Lr9, Lr10, Lr19, Lr26, Lr34, Lr37, Lr46, and Lr68, often in combination [7,10]. The most frequently detected genes were Lr37, Lr34, and Lr46, while Lr19, Lr68, Lr26, and Lr28 were less common. Some cultivars, like Keremet and Hisorok, carried four Lr genes, while others, including Aliya, Rasad, Reke, Mataj, Egana, and Almaly/Obri, carried three. Molecular screening identified 29 carriers of one Lr gene, 10 with two genes, 6 with three genes, and 2 with four genes. The combination of Lr37, Lr34, and Lr68 proved particularly effective, as carriers showed a low susceptibility index to diseases [8].
Yellow rust is a prevalent disease affecting winter wheat across Central Asia, including Kazakhstan. In favorable years, it often leads to widespread infection in crops [9,11,12,13,14,15]. This disease has become a significant constraint on winter wheat production in the region [9,11,12,16]. Historical data from Kazakhstan, Kyrgyzstan, and Uzbekistan indicate that the yellow rust population in Central Asia shares genetic similarities with populations in Western Asia [17]. In Central Asia, yellow rust typically exhibits high virulence to certain resistance genes, such as YrA, Yr2, Yr6, Yr7, Yr9, and Yr25. However, it is less virulent or not virulent to others like Yr5, Yr10, Yr15, Yr24, and YrSp [18].
In addition, due to the emergence and spread of the Ug99 virulent race, which creates devastating epidemics in the world [19,20,21,22], stem rust has become relevant for the regions of spring and winter wheat cultivation in Kazakhstan [9,23,24,25]. Currently, new virulent races of stem rust continue to endanger grain crops in the Central Asian region, particularly in Kazakhstan [26,27,28].
Farmers of Kazakhstan cultivate mainly local winter wheat cultivars Almaly, Bogarnaya 56, Zhetysu, Mereke 70, Sapaly, Steklovidnaya 24, Farabi, and others. These cultivars are notable for their high drought tolerance and adaptability to diverse environmental conditions. Some of these cultivars also exhibit field resistance to rust species. For instance, the Almaly cultivar contains the resistance genes Lr34/Yr18 [26,29], while Bogarnaya 56 carries Lr3a and Lr13 [30]. The Zhetysu cultivar also has Lr13 [30], Mereke 70 contains Yr10 and Yr18/Lr34 [29], and Sapaly has Lr3 along with powdery mildew resistance genes Pm3c and pm8 [31]. Steklovidnaya 24 is equipped with Lr3, Lr13, Pm3c, and pm8 [30,31]. Although many identified resistance genes, except for the Lr34/Yr18 complex and Yr10, have limited effectiveness both in Kazakhstan and globally, most domestic winter wheat cultivars are tolerant to rust damage. This tolerance means that despite moderate rust development, these cultivars generally do not experience significant reductions in grain productivity. It is possible that secondary resistance genes play a role in this resilience.
Kazakhstani and Russian researchers have employed molecular screening to identify promising wheat lines that possess a complex of rust-resistance genes. For instance, the Lr35/Sr39 gene complex was identified in wheat lines 304/14 and 125/14, while line 351/12 carried Lr37/Yr17/Sr38. Lines 89/14 and 386/13 were found to have both Lr35/Sr39 and Lr37/Yr17/Sr38. Additionally, lines 362/13, 116-10-4, and 211-10-10 contained Lr35/Sr39, and lines 239-10-17 and 56-10-13 had Lr37/Yr17/Sr38. Some lines, such as 319/14, 129/12, 366-13-5, and 385/12, were identified with multiple gene complexes, including Lr35/Sr39 and Lr37/Yr17/Sr38 [29]. In Kazakhstan, attractive gene combinations for rust resistance include Lr19/Sr25, Lr26/Sr31/Yr9/Pm8, Lr34/Yr18 APR, and Lr37/Yr17/Sr38. These combinations are proposed for use in developing wheat cultivars resistant to multiple rust species [29,32,33]. A study of 70 domestic winter wheat genotypes for yellow rust resistance found that 15 cultivars and 27 breeding lines were resistant. Molecular marker analysis revealed the presence of several genes and gene complexes, including Yr5, Yr10, Yr15, Yr17/Lr37/Sr38, and Yr18/Lr34. The Yr10 gene was the most common, identified in 22 genotypes, followed by Yr5 in 14 lines (20%), and Yr18 in 11 lines (15.7%). Yr15 was found in seven lines and the Yr17/Lr37/Sr38 complex in two genotypes [14].
Combining multiple resistance genes in a single wheat genotype can significantly enhance its defense against various diseases. For instance, integrating genes like Sr2/Lr27/Yr30 and Lr34/Yr18/Sr57 can substantially improve resistance to all three species of rust [34]. Strategically utilizing these pleiotropic genes, which confer resistance to multiple pathogens, in combination with other secondary genes is recommended for breeding rust-resistant wheat cultivars. From a practical standpoint, it is crucial to strike a balance between disease resistance and plant growth. While accumulating multiple resistance genes can enhance protection, it can also divert substantial energy from the host plant, potentially reducing yields [35]. Therefore, breeders must carefully manage the integration of these genes to ensure that the benefits of increased resistance do not compromise overall plant productivity.
Utilizing carriers of various minor Yr, Lr, and Sr genes can enhance the resistance of winter wheat to yellow, leaf, and stem rust in breeding programs. Modern wheat breeding techniques offer an effective approach to creating cultivars with strategic combinations of genes, which can contribute to developing durable resistance in the region. The objective of these studies was to assess the resistance of winter wheat cultivars and lines to leaf, yellow, and stem rust at both the adult plant and seedling stages. Additionally, the goal was to identify the genetic characteristics of resistant host plants in southern Kazakhstan, providing valuable insights for future breeding initiatives.

2. Results

2.1. Adult Plant Resistance Test

A collection of 55 promising winter bread wheat genotypes was assessed for rust resistance under field conditions in southern Kazakhstan from 2022 to 2024 (Table 1).
The evaluation revealed varying levels of resistance to leaf rust among the accessions. Notably, two Kazakhstani accessions, KIZ 90 and 20403-2, demonstrated high immunity to leaf rust, with no disease severity observed. Two Russian cultivars, Akhmat and Bezostaya 100, showed minimal infection, characterized by necrotic flecks with single pustules. Thirteen accessions exhibited moderate resistance, with disease severity ranging from 5% to 10%, while eighteen accessions displayed moderate susceptibility, with severity between 20% and 30%. The remaining genotypes had disease severity between 40% and 60%, which was lower than that of the susceptible standard, which had a severity of 80%.
In the assessment of yellow rust resistance, seven accessions (12.7%) displayed varying levels of resistance. Notably, two Kazakh lines, 21730-1 and 22372K, showed no symptoms of yellow rust (DS: 0%). Moderate resistance (DS: 5–20%) was observed in 14 accessions, while 11 accessions exhibited moderate susceptibility (DS: 20–30%). For other winter wheat genotypes, yellow rust severity ranged from 40% to 80%, with the susceptible standard being affected at 80%.
Different levels of stem rust resistance were identified in 17 accessions (30.9%). Kazakh cultivar KIZ 90 and lines 21730-1, 22372K, demonstrated a high level of field resistance to stem rust. Eleven accessions showed moderate resistance (DS: 5–20%) and twenty-three accessions displayed moderate susceptibility (DS: 20–30%). Two cultivars, Arap and Bakytzhan, and two lines, 20521-1 and 21203-11-3, were affected similarly to the susceptible standard, with disease severity ranging from 40% to 60%.
Ten accessions were found to be resistant or moderately resistant to all three types of rust. Cultivars KIZ 90, Alekseyich, Akhmat, Bezostaya 100, and lines 21730-1, 22372K exhibited very high resistance to leaf, yellow, and stem rust. Four genotypes—Adilet, 18410-1, 20197-17, and 22180-1—showed moderate resistance to the three rust types (disease severity: 10–20%). However, no accessions were completely immune (DS: 0%) to all three rust species.
Seven accessions were moderately resistant to two rust species. Cultivars Mereke 70 and Ilgor showed resistance to leaf and yellow rust, while lines 20403-2, 21144-4-1, 22315-1, 22353K, and D68CIMMYT were resistant to leaf and stem rust.

2.2. Seedling Rust-Resistance Test

Seedling infection type data for 20 promising winter wheat accessions inoculated with P. triticina, P. graminis, and P. striiformis pathotypes are presented in Table 2.
Leaf Rust. Only three accessions—Bezostaya 100, KIZ 90, and 18410-1—were found to be resistant to six P. triticina isolates and all of these accessions also showed resistance to leaf rust in field conditions. Notably, more accessions were resistant to Kazakh isolates of P. triticina compared to Russian ones. The leaf rust isolates used in the study varied in their virulence to several resistance genes, including Lr1, Lr2a, Lr2b, Lr2c, Lr9, Lr15, Lr19, and Lr26. A multi-pathogen test identified the Lr26 gene in three wheat accessions: Akhmat and lines 21730-1 and 22372K. These genotypes were resistant to PtK1-PtK4 isolates, which are avirulent to Lr26, but susceptible to the virulent PtK5 and PtK6 isolates. The tests revealed the absence of the Lr9 and Lr19 genes in the studied wheat collection. No accessions were found that were susceptible only to the PtK3 or PtK4 isolate and resistant to the other isolates tested. Additionally, the absence of the Lr2a, Lr2b, Lr2c, and Lr15 genes was confirmed. The P. triticina isolate PtK6 was distinct from others due to its avirulence to the Lr2a, Lr2b, Lr2c, and Lr15 genes. However, no accessions were identified that were resistant to the PtK6 isolate and susceptible to the other isolates tested.
High (R) and moderate resistance (MR) to stem rust was observed in three Kazakh lines—KIZ 90, 21730-1, and 22372K—and one Russian cultivar, Bezostaya 100. All these genotypes demonstrated resistance to stem rust under field conditions. The P. graminis isolates varied in their virulence to several resistance genes, including Sr7b, Sr8a, Sr9e, Sr9b, Sr11, Sr17, Sr24, Sr30, Sr36, and SrTmp. Notably, Kazakh isolates (PgK1 and PgK2) had fewer virulence alleles compared to Russian isolates. Cultivar Adilet and lines 18410-1, 20521-1, 21203-11-3, and 22353K were resistant to two Kazakh P. graminis isolates (PgK1 and PgK2). Additionally, cultivars Akhmat, Euclide, and SWW1-904 were resistant to the PgK2 isolate, while line 22180-1 was also resistant to PgK2. These isolates differed in their avirulence to the Sr7b, Sr9e, Sr9b, Sr36, and SrTmp genes. The multi-pathogen tests revealed the absence of the Sr24 gene in the studied wheat collection. No accessions were found that were susceptible only to the PgK4 isolate and resistant to the other isolates tested.
The number of accessions resistant to yellow rust was notably lower compared to those resistant to stem rust and leaf rust. Only two accessions, Akhmat and line 18410-1, showed resistant reactions to all P. striiformis isolates. However, these accessions displayed a moderate level of resistance to yellow rust under field conditions. Resistance to two Kazakh isolates of P. striiformis (Pst_1, Pst_2) was observed in 11 wheat accessions, while four accessions were resistant to only one of these isolates. Among them, six accessions—22372K, 22353K, 21203-11-3, 20197-17, KIZ 90, and Bezostaya 100—demonstrated resistance in the field. The number of genotypes resistant to Kazakh isolates (Pst_1 and Pst_2) was higher than those resistant to Russian isolates (Pst_3 and Pst_4), although the isolates did not differ significantly in virulence. All isolates were avirulent to Yr5, Yr10, and YrSP, but virulent to Yr8. The main difference among them was their virulence or avirulence to genes Yr1, Yr7, Yr9, and Yr24 (=Yr26). Following multi-pathogen testing, the Yr9 gene was identified in line 21730-1, which was resistant to the Pst_1 isolate avirulent to Yr9, but susceptible to other isolates with virulence to this gene. No other Yr genes were detected in the wheat accessions tested during the multi-pathogen test.
Overall, no lines were identified that were resistant to all rust species in the seedling resistance study. However, two accessions, Bezostaya 100 and KIZ 90, showed seedling resistance to both leaf and stem rust and were also resistant in the field.

2.3. Identification of Rust-Resistance Genes Using Molecular Markers

Molecular markers were utilized to identify a range of leaf rust-resistance genes, including Lr1, Lr3, Lr9, Lr10, Lr19, Lr20, Lr24, Lr25, Lr26, Lr28, Lr29, Lr34, Lr37, Lr41(39), Lr47, Lr51, LrAsp, and Lr6Agi2. Additionally, yellow rust-resistance genes Yr9, Yr17, and Yr18, as well as stem rust-resistance genes Sr2, Sr15, Sr24, Sr25, Sr26, Sr28, Sr31, Sr36, Sr38, Sr39, and the 1AL.1RS translocation, were identified.
In the studied collection, specific leaf rust-resistance genes detected included Lr1, Lr3, Lr26, and Lr34. Yellow rust-resistance genes identified were Yr9 and Yr18, while stem rust-resistance genes included Sr31 and Sr57, along with the 1AL.1RS translocation. The most frequently identified rust genes, either alone or in combination, were Lr34, Yr18, and Sr57, found in eight accessions. The Lr3 gene was postulated in five accessions and the Lr1 gene in four accessions. The 1BL.1RS rye translocation, carrying the Lr26, Yr9, and Sr31 genes, was detected in four accessions, while the 1AL.1RS rye translocation was found in one cultivar (Table 2).

3. Discussion

Rust species are a significant challenge in wheat production in Kazakhstan, where epidemics typically occur every 4 years. In years with sufficient rainfall in April and May, diseases spread rapidly among winter wheat crops in the south and southeast of the country. Currently, there is limited information on the resistance of wheat cultivars from Central Asia at both the adult plant and seedling stages. To address this gap, our study aimed to evaluate the resistance of adult plants in the field and wheat seedlings in controlled environments. Additionally, we sought to identify the primary and secondary Lr, Yr, and Sr genes responsible for resistance to leaf, yellow, and stem rust, respectively. Our research followed the recommendations of prominent scientists to breeders, phytopathologists, and geneticists working in Central Asia. We assessed a collection of wheat cultivars in field hotspots and in greenhouses to determine seedling resistance to specific rust races. This approach helps identify cultivars that demonstrate resistance to diseases, which can then be transferred to susceptible but locally adapted cultivars through simple crosses. Any breeding program in the region can utilize this straightforward breeding methodology to achieve long-term rust control [36].
In the southeast of Kazakhstan, the years 2022 and 2024 were conducive to the development of rust species in the field, with a rainy and cool spring contributing to the strong spread of leaf, yellow, and stem rust on susceptible cultivars. Yellow rust was particularly prevalent among the studied genotypes, highlighting its relevance in southern Kazakhstan and throughout Central Asia compared to leaf and stem rust [9,11,12,16]. When evaluating the resistance of winter wheat against this infectious backdrop, several cultivars and lines stood out for their resistance to all three rust species. Cultivars KIZ 90 (Kazakhstan), Alekseyich, Akhmat, Bezostaya 100 (Russia), and breeding lines 21730-1 and 22372K were among the most resistant. Four genotypes—Adilet, 18410-1, 20197-17, and 22180-1—displayed moderate resistance to the three rust species. However, no samples were found to be completely immune to all three rust species. Seven samples were moderately resistant to two rust species: cultivars Mereke 70 and Ilgor to leaf and yellow rust, and lines 20403-2, 21144-4-1, 22315-1, 22353K, and D68CIMMYT to leaf and stem rust. The resistance of these cultivars and lines was generally categorized as moderate resistance. Notably, genotypes Bezostaya 100, KIZ 90, 21730-1, and 22372K showed an immune response to the stem rust population in field conditions (Table 1) and to individual races in greenhouse conditions (Table 2).
In our research, we focused not only on primary resistance genes with significant effects but also on secondary genes, particularly those that confer resistance to multiple rust species. This approach is crucial for ensuring long-term resistance, as combining several minor genes can provide a level of “near immunity” to rust diseases in wheat [37]. Additionally, integrating multiple resistance genes involved in various defense mechanisms enhances resistance to different rust species. For instance, combining Lr34/Yr18/Sr57 significantly improves resistance to all three types of rust [34]. Our molecular genetic study of 20 winter wheat genotypes used molecular markers to identify 18 Lr genes, 3 Yr genes, and 10 Sr genes, as well as to determine the 1AL.1RS translocation. The results showed that in eight genotypes, resistance to the three rust species is attributed to a combination of the Lr34, Yr18, and Sr57 genes (Table 2). This gene complex provides partial and race-independent resistance to a variety of pathogens, including all three types of wheat rust. Currently, the Lr34/Yr18/Sr57 gene complex is widely utilized in breeding programs. Leaf Tip Necrosis in wheat serves as a visual marker for this gene complex, facilitating selection [38]. The Yr18 gene is noted for providing long-term stability, contributing to grain yields ranging from 36% to 58%, depending on the year and sowing date, even with a significant prevalence of yellow rust [39]. In Egyptian wheat cultivars like Sakha 94 and Shandaweel 1, which exhibit slow yellow rust development in adult plants, the presence of the Yr18 gene associated with Adult Plant Resistance has been confirmed using phenotypic and genotypic markers [40]. Furthermore, the Lr34 gene, combined with other secondary additive genes, is effective in providing long-term resistance to leaf rust in wheat cultivars during adult plant growth [41,42]. Overall, the Lr34, Yr18, and Sr57 genes have been instrumental in enhancing rust resistance globally, but ongoing efforts are needed to maintain their effectiveness against evolving rust populations.
Another significant gene complex, comprising Lr26, Yr9, and Sr31, has been extensively utilized in wheat breeding programs [43]. This complex was introduced into the wheat genome through wheat-rye translocation chromosomes. Using molecular markers, we identified these genes in the genotypes of the Bezostaya 100 and KIZ 90 cultivars, as well as in breeding lines 21730-1 and 22372K. Although the Sr31 resistance gene has lost effectiveness in regions where Ug99 racial variants are prevalent, cultivars carrying this gene still exhibit resistance to stem rust in many countries [9,19,20,44]. We also detected a 1AL.1RS rye translocation in the Akhmat cultivar. A similar translocation was found in a CIMMYT line (identification number 8248316). In both the Akhmat cultivar and this CIMMYT line, stem rust severity was limited to 5% or less [45]. This highlights the ongoing utility of these genetic resources in maintaining resistance to stem rust.
Winter wheat cultivars and breeding lines selected in southern Kazakhstan, which contain the Lr34, Yr18, Sr57, as well as Lr26, Yr9, and Sr31 gene complexes, are highly valuable for providing partial and long-term resistance to rust species. When combined with other primary and secondary genes, these complexes can effectively inhibit the emergence of virulent races of leaf, yellow, and stem rust. Our findings align with those reported by other researchers, highlighting the importance of these gene combinations in enhancing rust resistance [34,46,47,48,49,50,51,52,53].

4. Materials and Methods

4.1. Plant Material

The field rust-resistance study involved 55 advanced winter wheat genotypes (Triticum aestivum L.), comprising 35 from Kazakhstan, 7 from Uzbekistan, 5 from Russia, 3 from Kyrgyzstan, 1 from France, and 4 from CIMMYT. Twenty promising accessions, including fifteen from Kazakhstan, two each from Russia and CIMMYT, and one from France, were selected for seedling resistance and molecular studies (Table S1).

4.2. Assessment of Field Response to Leaf, Yellow, and Stem Rust

In the experimental field nursery of the Kazakh Research Institute of Agriculture and Plant Growing (N 43°13′09″ E 76°41′17″), experiments were conducted to study the resistance of winter wheat to rust species. For this purpose, the seeds of winter wheat cultivars and lines were sown using the standard method (1-m row with a distance between rows of 20 cm). The susceptible standards (cultivars Bogarnaya 56 for leaf rust, Morocco for yellow rust, and Bakytzhan for stem rust), which were spreader rows of infection, were used as a susceptible indicator. Seeds of their cultivars were sown every 20 rows to ensure uniform spread of leaf, yellow, and stem rust infection. Inoculation of the studied genotypes was carried out with a P. triticina, P. striiformis, and P. graminis population with talc in a ratio of 1:100, with a load of 20 mg of spores per sq. m. The inoculum of the each of population was a mixture of uredospores from a local population of the fungus, previously collected from wheat crops in the experimental area. The first assessment of the development of the disease was carried out at the beginning of its manifestation, subsequent ones—with an interval of 10–12 days until the milky-waxy ripeness of the grain. The main phytopathological parameters for assessing genotypes for resistance to the rust pathogens were: infection type (IT) and disease severity (DS, %). The IT was determined using the recommended CIMMYT score [54]: I (immune), R (resistant), MR (moderately resistant), MS (moderately susceptible), S (susceptible). The DS of plants (%) was determined using the modified Cobb scale [55]. The coefficient of infection (CI) for rust development was calculated by multiplying the constant values of IT by the DS. Constant values of infection types were used: I = 0.0; R = 0.2; MR = 0.4; M = 0.6; MS = 0.8 and S = 1.0 [56]. The CI value is used for grouping accessions by resistance to rust species; a CI in the range of 0–2.0 means highly resistant genotypes, 8.0–24.0 are moderate resistant, 40.0 or more belongs to the susceptible group.
Weather conditions in 2022 and 2024 were more favorable for rust development than in 2023. The annual precipitation for 3 years ranged from 469.0 to 758.9 mm (Table 3).
Weather conditions in Almaty region in 2022 were moderately favorable for the development of three rust species. On susceptible standards, the maximum severity of leaf, yellow, and stem rust were 60%, 80%, and 40%, respectively (Table 3).
In 2023, insufficient rainfall in May (43.4 mm) and especially in June (4.3 mm) resulted in disease depression on wheat. No symptoms of yellow rust and stem rust were detected. Moderate development of leaf rust was observed for eight accessions: 20933-1, 20982-2 (DS: 5%), Almaly, Dulaty, Egemen, Steklovidnaya 24, Vavilov, D580CIMMYT (DS: 10%).
Weather conditions in 2024 were highly favorable for the development of three species of rust. In April, the average monthly air temperature was 12.8 °C and the total precipitation was 111.3 mm. In May, the average air temperature was 17.6 °C and the sum of precipitation was 121.2 mm, which is 1.2 times more than the multi-year indicator. Cool night temperatures with an average of 12.4 °C favored the successful development of yellow rust. Leaf and yellow rust severity on susceptible standards reached 80% and stem rust 60%.

4.3. Seedling Rust-Resistance Tests

Two research centers carried out seedling resistance studies (Kazakh Research Institute of Agriculture and Plant Growing, Almalybak, Kazakhstan) and All-Russian Institute of Plant Protection, St. Petersburg, Russia).
Leaf rust infection at the seedling stage was evaluated for six P. triticina isolates with different virulence/avirulence combinations, yellow rust infection for four P. striiformis isolates, and stem rust infection for five P. graminis isolates. For leaf rust, Thatcher’s isogenic lines (TcLr) with genes Lr: 1, 2a, 2b, 2c, 3a, 3bg, 3ka, 9, 11, 14a, 15, 16, 17, 20, 24, 26, 29, and 30 were used in both laboratories. In addition, the TcLr25 line was added in Kazakhstan to characterize the virulence of P. triticina isolates and TcLr10, TcLr14b, TcLr18, and TcLr28 lines and breeding accessions with Lr47 and Lr51 were added in Russia [57]. Based on the North American system of nomenclature [58]. Kazakh isolates Pt_1 and Pt_2 belonged to the TGT and KHT races and Russian isolates PtK1-K4 to TLT, TGT, THT, and MHT races correspondingly. Virulence/avirulence was determined with the following differential sets: group I: Lr1, Lr2a, Lr2c and Lr3a; group II: Lr9, Lr16, Lr24 and Lr26; group III: Lr3ka, Lr11, Lr17 and Lr30.
Avocet lines with genes Yr: 1, 5a, 6, 7, 8, 9 10, 15, 17, 24, 27, and 5b (=YrSp) and 15 cultivars from International (Chinese 166, Lee, Heines Kolben, Vilmorin 23, Moro, Strubes Dickkopf, Suwon 92/Omar) and European (Hybrid 46, Reicherberg 42, Heines Peko, Nord Desprez, Compair, Carstens V, Spalding Prolific, Heines VII) differential sets were used for determination of virulence of P. striiformis isolates used in multi-pathogen test [59]. The infection type (IT) of wheat seedlings to yellow rust was determined according to the Gassner and Straib scale [60].
Designation of races for P. graminis was made using the following international differential sets; group I: Sr5, Sr21, Sr9e and Sr7b; group II: Sr9a11, Sr6, Sr8a and Sr9g; group III: Sr36, Sr9b, Sr30 and Sr17; group IV: Sr9a, Sr9d, Sr10 and SrTmp; and group IV: Sr24, Sr31, Sr38, and SrMcN [61]. Accordingly, Kazakh isolates PgK1 and PgK2 were designated as THMTF and QHHSF races and Russian isolates PgK3-K5 as TTTTF, TTTTP, and TTSTF races (Table 4).
For evaluation of seedling resistance to three rust species seeds of 20 studied wheat seeds accessions (from 5 to 10 seeds) were sown in plastic pots with a volume of 200 mL. Spores of a single-pustule isolates each rust species were mixed with a light mineral oil (NOVEC 7100) and sprayed onto the 10-day old wheat plants (seedlings with the first leaf fully unfolded) for leaf and stem rust-resistance studies and 12–14-day old plants (second leaf appearance) for yellow rust-resistance study. Wheat seedlings inoculated by P. triticina and P. graminis isolates, were incubated in a dark humid chamber for 16–24 h at 20–22 °C, and inoculated by P. striiformis isolates at 10 °C. Then wheat plants were incubated in greenhouse boxes with photoperiod 16 h day (10–15 thousand lux)/8 h night at 20–22 °C for leaf and stem rust. The plants inoculated by yellow rust isolates transferred to a growth chamber (Environmental Test Chamber MLR-352H, Sanyo Electric Co., Ltd., Osaka, Japan) with 16:8 h L:D photoperiod at 16 and 10 °C, respectively. Infection types were assessed 12 days after the incubation for leaf and stem rust (Mains and Jackson and Stakman and Levin scales correspondingly) [62,63] and after 16–18 days for yellow rust (Gassner and Straib scale) on a five-point scale [60]. Isolates with infection types 0–2 and 3–4 were assumed to be avirulent and virulent, respectively.

4.4. Identification of Lr, Sr, and Yr Genes Using Molecular Markers

Identification of rust-resistance genes was carried out at the All-Russian Institute of Plant Protection (St. Petersburg). Molecular markers were used for the identification of 18 Lr genes (Lr1, Lr3, Lr9, Lr10, Lr19, Lr20, Lr24, Lr25, Lr26, Lr28, Lr29, Lr34, Lr37, Lr41(39), Lr47, Lr51, LrAsp, and Lr6Agi2), 3 Yr genes (Yr9, Yr17 and Yr18), 10 Sr genes (Sr2, Sr15, Sr24, Sr25, Sr26, Sr28, Sr31, Sr36, Sr38, Sr39), and 1AL.1RS translocation carries the stem rust-resistance gene SrR, but no known leaf and yellow resistance genes [64]. The list of used molecular markers of Lr, Yr, and Sr genes is presented in Table 5.
In the leaf and yellow rust study, DNA was extracted according to Dorokhov and Cloquet [85]. PCRs were performed using a thermocycler (C1000, BioRad, Hercules, CA, USA). PCR mixture (20 mL) contained 100–150 ng of genomic DNA, 2 units of Taq DNA polymerase, 1× PCR buffer (10 mM Tris HCL), 2.5 mM of MgCl2, 100 mM of each dNTP, and 10 mM of each primer. The recommended PCR protocol (Table 5) was used in amplifications. In stem rust study, the PCR mixture BioMaster HS-Taq PCR-Color (BIOLABMIX LLC, Novosibirsk, Russia) and the following amplification conditions were applied: 95°—5 min, 35 cycles (95°—20 s, annealing temperature—30 s, 72°—1 min), and 72°—5 min was used. The annealing temperature was individual for each pair of primers. For the Sr2 gene marker, csSr2, the following PCR mixture was used: 20 µL of the reaction mixture; bidistilled H2O—17.6 µL; a mixture of dNTPs (25 mM)—0.4 µL; primer R (10–15 pmol)—0.5 µL; primer F (10–15 pmol)—0.5 µL; 10× PCR buffer—2.5 µL; MgCl2 (50 mM)—1 µL; Taq polymerase (5U)—0.5 µL; and genomic DNA—2 µL. The amplification conditions were as follows: 94°—4 min 30 s, 45 cycles (94°—1 min, 60°—1 min, 72°—2 min), 72°—10 min. After PCR, the amplification products were treated with restriction endonuclease BspHI. PCR products were separated on 1.5 to 3.0% agarose gels (depending on gene product size) and visualized under UV light using the digital gel imaging system (GelDocGo, BioRad, Hercules, CA, USA).

5. Conclusions

In our research, a collection of 55 winter wheat cultivars and breeding lines in southern Kazakhstan exhibited diverse reactions to natural populations of leaf, yellow, and stem rust. During the favorable disease development conditions in 2022 and 2024, 20 wheat cultivars and lines with Adult Plant Resistance were selected. Using specific virulent races from Kazakhstan and Russia, the resistance of 12 genotypes at the seedling stage was confirmed. Molecular methods identified eight sources carrying the Lr34, Yr18, and Sr57 genes, four sources with Lr26, Yr9, and Sr31, as well as sources with Lr1, Lr3, and the 1AL.1RS translocation. The utilization of winter wheat cultivars containing secondary resistance genes is highly valuable for providing partial and long-term resistance to leaf, yellow, and stem rust. This approach helps mitigate the emergence of new virulent races in the region, contributing to sustainable wheat production.

Supplementary Materials

The following supporting information can be downloaded at: https://www.mdpi.com/article/10.3390/plants14071146/s1, Table S1: Pedigrees and origin of cultivars and breeding lines of winter wheat (Triticum aestivum L.) taken for experiments. Table S2: Dynamics of leaf, yellow, and stem rust severity on winter wheat cultivars and breeding lines in 2024, Almaty region, Kazakhstan; Table S3: Meteorological data in the Almaty region of Kazakhstan for 2022, 2023, and 2024; Figure S1: Photos of the development of leaf, yellow, and stem rust on winter wheat cultivars and breeding lines in the Almaty region of Kazakhstan in 2024; Figure S2: Results of the molecular analysis of the study of Lr, Yr, and Sr resistance genes.

Author Contributions

Conceptualization, S.R. and E.G.; methodology, S.R., E.G., O.B. and E.S.; formal analysis, E.S. and A.K.; validation, S.R. and E.G.; investigation, S.R., E.G., O.B., E.S., A.A. and G.A.; writing—original draft preparation, S.R.; writing—review and editing, S.R., E.G., O.B. and A.K.; visualization, E.G. and A.K.; supervision, S.R. and R.U.; funding acquisition, S.R. All authors have read and agreed to the published version of the manuscript.

Funding

This research was funded by the Science Committee of the Ministry of Science and Higher Education of the Republic of Kazakhstan, grant project IRN AP19677043 “Selection of winter wheat genotypes with group resistance to rust species”.

Data Availability Statement

The data presented in the study are available on request from the corresponding author.

Conflicts of Interest

The authors declare no conflicts of interest. The funders had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript; or in the decision to publish the results.

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Table 1. Rust infection type, disease severity, and coefficient of infection of winter wheat accessions in the field in Southern Kazakhstan in 2022–2024.
Table 1. Rust infection type, disease severity, and coefficient of infection of winter wheat accessions in the field in Southern Kazakhstan in 2022–2024.
No.EntryRust Infection Type (IT), Disease Severity (DS), and Coefficient of Infection (CI)
P. triticinaP. striiformis f. sp. triticiP. graminis f. sp. tritici
IT *DS, % **CI ***IT *DS, % **CI ***IT *DS, % **CI ***
1.AdiletMR208.0MR208.0R5–102.0
2.AlmalyMR10–208.0MR208.0MR10–208.0
3.AmanatMS20–3024.0S40–6060.0MS20–3024.0
4.ArapMS20–3024.0MR-MS20–3024.0S4040.0
5.BakytzhanMS20–3024.0MR-MS20–3024.0S40–6060.0
6.DimashMS20–3024.0MR10–208.0MS20–3024.0
7.DulatyMR10–208.0R-MR5–104.0MR10–208.0
8.Egemen 20MS-S30–4040.0MS-S30–4040.0MS20–3024.0
9.FarabiMS-S30–4040.0MS20–3024.0MS20–3024.0
10.Kazakhstan 10MS-S30–4040.0MR-MS20–3024.0MR-MS20–3024.0
11.KIZ 90I00.0I-R0–51.0I00.0
12.Mereke 70R-MR5–208.0R5–102.0MR-MS20–3024.0
13.MomyshulyMS20–3024.0MR-MS20–3024.0MR-MS20–3024.0
14.NesipkhanMS20–3024.0MR10–208.0MR-MS20–3024.0
15.Pamyat 47MS20–3024.0S60–8080.0MR-MS20–3024.0
16.SapalyMR-MS20–3024.0MR-MS10–3024.0R-MR5–104.0
17.Steklovidnaya 24MS-S30–4040.0MS-S30–4040.0MS20–3024.0
18.Talimi 80MR-MS20–3024.0MR10–208.0MR10–208.0
19.VavilovMS-S30–4040.0MR-MS20–3024.0MR-MS20–3024.0
20.ZhetysuMS-S30–4040.0MS-S30–4040.0MS20–3024.0
21.18410-1R-MR5–208.0R-MR5–208.0MR10–208.0
22.18411-1MS20–3024.0MS-S30–4040.0R5–102.0
23.20197-17R-MR5–208.0R-MR5–208.0R-MR5–208.0
24.20403-2I00.0MR-MS20–3024.0R1–51.0
25.20521-1MS3024.0S4040.0S4040.0
26.20933-1MS20–3024.0MR-MS20–3024.0R-MR5–208.0
27.20982-2MS20–3024.0R-MR5–104.0MR10–208.0
28.21144-4-1R5–102.0MR-MS20–3024.0R-MR5–104.0
29.21203-11-3MS20–3024.0S4040.0S4040.0
30.21266-3MR10–208.0MR10–208.0R-MR5–104.0
31.21730-1R5–102.0I00.0I00.0
32.22180-1MR10–208.0MR10–208.0MR10–208.0
33.22315-1R5–102.0MR-MS20–3024.0I-R0–51.0
34.22353KR-MR1–208.0MR10–2024.0R1–102.0
35.22372KR1–51.0I00.0I00.0
36.AlekseyichR1–102.0I-R0–50.5R1–51.0
37.AkhmatI-R0–10.2R1–51.0R1–51.0
38.Bezostaya 100I-R0–10.2R1–102.0I00.0
39.GromR1–102.0MR10–208.0MS20–3024.0
40.GurtMS20–3024.0S60–8080.0MR10–208.0
41.BardoshMS20–3024.0MR5–208.0MR-MS10–3024.0
42.EzozR1–102.0S60–8080.0MR-MS20–3024.0
43.IlgorR1–102.0R5–102.0MR-MS10–3024.0
44.KayraktoshR1–102.0MS-S30–6060.0MR-MS10–3024.0
45.Ok marvaridMS-S30–4040.0MS-S30–4040.0MR-MS20–3024.0
46.PahlavonR-MR5–208.0MS-S30–4040.0MR-MS20–3024.0
47.TespisharMS20–3024.0S60–8080.0MR10–208.0
48.AjaraMR208.0S60–8080.0MR-MS10–3024.0
49.AsylMR208.0MR-MS10–3024.0MR-MS20–3024.0
50.IntensivnayaMS-S30–6060.0MS-S30–4040.0MR-MS10–3024.0
51.D68CIMMYTR5–102.0S4040.0R-MR5–104.0
52.D580CIMMYTMS-S30–6060.0S4040.0MR10–208.0
53.D952CIMMYTMS20–3024.0S4040.0MS20–3024.0
54.SWW 1/904MS20–3024.0S4040.0MS20–3024.0
55.EuclideMR10–208.0MS20–3024.0MS20–3024.0
(St1) 1Bogarnaya 56 S60–8080.0------
(St2) 2Morocco ---S40–8080.0---
(St3) 3Bakytzhan ------S40–6060.0
* IT—infection type (I—immune, R—resistant, MR—moderately resistant, MS—moderately susceptible, S—susceptible), ** DS—disease severity (%), *** CI—coefficient of infection, 1, 2, 3—Susceptible standards for leaf rust, yellow rust, and for stem rust, respectively.
Table 2. The results of the assessment of winter wheat cultivars to the yellow, leaf, stem rust races at the seedling stage and the identification of resistance genes.
Table 2. The results of the assessment of winter wheat cultivars to the yellow, leaf, stem rust races at the seedling stage and the identification of resistance genes.
EntryReaction Type to Rust Isolates at the Seedling Stage *Identified Resistance GenesField Resistance **
Leaf RustYellow RustStem Rust Leaf RustYellow RustStem Rust
PtK1PtK2PtK3PtK4PtK5PtK6Pst_1Pst_2Pst_3Pst_4PgK1PgK2PgK3PgK4PgK5
Adilet 1+0; 13–4 3–43–43–42333223+34Lr3  Lr34  Yr18 Sr57MR 20MR 20R 5–10
Almaly433–43–43–43–4443333433-Lr34  Yr18 Sr57MR 10–20MR 20MR 10–20
Akhmat1+10–20–13–43–4220–1;232443Lr1 1Al.1RS I-R 0–1R 1–5R 1–5
Amanat32+3–43–43–43–4333343433-MS 20–30S 40-60MS 20–30
Bezostaya 100000–10–10–10–100332+2+21–21–2Lr26 Lr34 Yr9 Yr18 Sr31 Sr57I-R 0–1R 1–10I 0
D952CIMMYT223–43–43–43–4003343+444-MS 20–30S 40MS 20–30
Dulaty333–43–43–43–43332–3343-44Lr34 Yr18 Sr57MR 10–20R-MR 5–10MR 10–20
Egemen 20233–43–43–43–42+233334+34-MS-S 30–40MS-S 30–40MS 20–30
Euclide21+3–43–43–43–4202–33323-33-Lr1MR 10–20MS 20–30MS 20–30
KIZ 900; 120–10;0;0;20330; 12-21-22Lr1 Lr3 Lr26 Yr9 Sr31I 0I-R 0–5I 0
Steklovidnaya24343–43–43–43–4443344334-MS-S 30–40MS-S 30–40MS 20–30
SWW 1/9040; 103–43–43–43–4320–1;332+444-MS 20–30S 40MS 20–30
18410-10; 11+0;0–1;0–1;0–1;+110;00; 1133-3Lr34  Yr18 Sr57R-MR 5–20R-MR 5–20MR 10–20
20197-17223–43–43–43–4122–3333+434Lr34  Yr18 Sr57R-MR 5–20R-MR 5–20R-MR 5–20
20521-1023–43–43–43–42433224+3+4-MS 30S 40S 40
21203-11-3323–43–43–43–4023322443Lr3MS 20–30S 40S 40
21730-1100–10–13–43–4033300; 122+1-2Lr3 Lr26 Yr9 Sr31R 5–10I 0I 0
22180-1223–43–43–43–400332+3344Lr34 Yr18 Sr57MR 10–20MR 10–20MR 10–20
22353K21+3–43–43–43–420332243+4Lr1 Lr3 Lr34 Yr18 Sr57R-MR 1–20MR 10–20R 1–10
22372K1+1+, 20–10–13–43–40033122-1–22-Lr3 Lr26 Yr9 Sr31R 1–5I 0I 0
* Reaction types for seedlings were 0, 0; 1, 2 for resistance and 3, 4 for susceptibility; «;» hypersensitive flecks; «+» more than average for the class. ** Rust severity in the field: infection type, IT (I—immune, R—resistant, MR—moderately resistant, MS—moderately susceptible, S—susceptible) and disease severity, DS (%).
Table 3. Precipitation and average air temperature from sowing to harvesting of winter wheat in the Almaty region in 2022–2024.
Table 3. Precipitation and average air temperature from sowing to harvesting of winter wheat in the Almaty region in 2022–2024.
YearIndicatorMonths From Sowing to Harvesting of Winter Wheat
OctoberNovemberDecemberJanuaryFebruaryMarchAprilMayJuneJuly
2021–2022Precipitation, mm.77.741.314.016.333.9168.646.8145.435.915.1
Air temperature, °C7.91.11.30.00.85.816.719.024.326.5
2022–2023Precipitation, mm.42.2128.214.036.934.061.268.243.44.336.6
Air temperature, °C11.02.9−4.6−6.90.38.411.917.224.627.1
2023–2024Precipitation, mm.70.967.864.938.843.6135.5111.3121.219.785.2
Air temperature, °C13.46.8−1.0−1.2−4.05.412.817.624.525.0
Table 4. Virulence/avirulence profile of P. triticina, P. striiformis, and P. graminis isolates.
Table 4. Virulence/avirulence profile of P. triticina, P. striiformis, and P. graminis isolates.
Isolate OriginationVirulence to GenesAvirulence to Genes
Puccinia triticina
Pt_1_TGTKazakhstan, Kostanay, 2021Lr: 1, 2a, 2b, 2c, 3a, 3bg, 3ka, 11, 14a, 16, 17, 20, 30Lr: 9, 19, 24, 25, 26, 29
Pt_1_ KHTKazakhstan, Almaty, 2022Lr: 2a, 2b, 2c, 3a, 3bg, 3ka, 11, 14a, 16, Lr17, 26, 30Lr: 1, 9, 15, 19, 24, 25, 26, 29
PtK1Russia, Chelyabinsk, 2022Lr: 1, 2a, 2b, 2c, 3a, 3bg, 3ka, 9,10, 14a, 14b, 15, 17, 18, 20, 30Lr: 19, 16, 24, 26, 28, 47, 51
PtK2Russia, Saratov, 2021Lr: 1, 2a, 2b, 2c, 3a, 3bg, 3ka, 10, 14a, 14b, 15, 16, 17, 18, 19, 20, 30Lr: 9, 24, 26, 28, 29, 47, 51
PtK3Russia, Novosibirsk, 2021Lr: 1, 2a, 2b, 2c, 3a, 3bg, 3ka, 10, 14a, 14b, 15, 16, 17, 18, 20, 26, 30Lr: 9, 19, 24, 28, 29, 47, 51
PtK4Russia, Dagestan, 2023Lr: 1, 2c, 3a, 3bg, 3ka, 10, 14a, 14b, 16, 17, 18, 20, 26, 30Lr: 2a, 2b, 9, 15, 19, 24, 28, 29, 47, 51
Puccinia striiformis f. sp. tritici
Pst_1Kazakhstan, Taraz, 2022Yr: 1, 6, 7, 8, 12, 18, 27Yr: 5, 9, 10, SP, 26
Pst_2Kazakhstan, Almaty, 2022Yr: 1, 6, 8, 9, 18, 26Yr: 5, 7, 10, 12, SP, 27
Pst_3Russia, Novosibirsk, 2021Yr: 1, 2, 3, 6, 8, 9, 27, SD Yr: 4, 5, 7, 10, 15, 17, 24, SP, ND
Pst_4Russia, St. Petersburg, 2022Yr: 2, 3, 4, 6, 8, 9, 27Yr: 1, 5, 7, 10, 15, 17, 24, SP, SD, ND
Puccinia graminis f. sp. tritici
PgK1Kazakhstan, Kostanay, 2021Sr: 5, 6, 7b, 9a, 9d, 9g, 9e, 10, 17, 21, 36, 38, Tmp, McNSr: 8a, 9b, 11, 24, 30, 31
PgK2Kazakhstan, Almaty, 2022Sr: 5, 6, 9a, 9b, 9d, 9g, 10, 17, 21, 38, McNSr: 7b, 8a, 9e, 11, 24, 30, 31, 36, Tmp
PgK3Russia, Saratov, 2022Sr: 5, 21, 9e, 7b, 11, 6, 8a, 9g, 36, 9b, 30, 17, 9a, 9d, 10, Tmp, 38, McN Sr: 24, 31
PgK4Russia, Tatarstan, 2023Sr: 5, 21, 9e, 7b, 11, 6, 8a, 9g, 36, 9b, 3, 17, 9a, 9d, 10, Tmp, 24, 38, McN Sr: 31
PgK5Russia, Saratov, 2022Sr: 5, 21, 9e, 7b, 11, 6, 8a, 9g, 36, 9b, 30, 9a, 9d, 10, Tmp, 31, 38, McNSr: 17, 24, 31
Table 5. Molecular markers for the identification of the Lr, Yr, and Sr genes.
Table 5. Molecular markers for the identification of the Lr, Yr, and Sr genes.
GeneMarkerAllele Size, bpReferences
Lr1WR003 F/R760[65]
Lr3aXmwg798365[66]
Lr9SCS5550[67]
Lr10F1.2245/Lr10-6/r2310[68]
Lr25Lr25F20/R191800[69]
Lr28SCS421570[70]
Lr29Lr29F24900[69]
Lr41 (39)GDM35190[71]
Lr47PS10282[72]
Lr51S30-13L/AGA7-759783, 422[73]
Lr66 (Asp)S13-R16695[74]
Lr19, Sr25SCS265 512[75]
Lr20, Sr15STS638542[76]
Lr24, Sr24Sr24 ≠ 12, Sr24 ≠ 50 500, 200[77]
Lr26, Sr31, Yr9SCM9207 (1BL.1RS), 228 (1AL.1RS)[78]
Lr34, Sr57, Yr18csLV34150[79]
Lr37, Sr38, Yr17Ventriup/LN2259[80]
Lr_Yr6Agi2MF2/MR1r2347[81]
Sr2csSr2172[82]
Sr28wPt-7004-PCR, Xwmc332194, 214, 217, 220[83]
Sr26Sr26#43207[77]
Sr36Xwmc477, Xstm773-2190, 155[84]
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Rsaliyev, S.; Gultyaeva, E.; Baranova, O.; Kokhmetova, A.; Urazaliev, R.; Shaydayuk, E.; Abdikadyrova, A.; Abugali, G. Characterizing the Genetic Basis of Winter Wheat Rust Resistance in Southern Kazakhstan. Plants 2025, 14, 1146. https://doi.org/10.3390/plants14071146

AMA Style

Rsaliyev S, Gultyaeva E, Baranova O, Kokhmetova A, Urazaliev R, Shaydayuk E, Abdikadyrova A, Abugali G. Characterizing the Genetic Basis of Winter Wheat Rust Resistance in Southern Kazakhstan. Plants. 2025; 14(7):1146. https://doi.org/10.3390/plants14071146

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Rsaliyev, Shynbolat, Elena Gultyaeva, Olga Baranova, Alma Kokhmetova, Rahim Urazaliev, Ekaterina Shaydayuk, Akbope Abdikadyrova, and Galiya Abugali. 2025. "Characterizing the Genetic Basis of Winter Wheat Rust Resistance in Southern Kazakhstan" Plants 14, no. 7: 1146. https://doi.org/10.3390/plants14071146

APA Style

Rsaliyev, S., Gultyaeva, E., Baranova, O., Kokhmetova, A., Urazaliev, R., Shaydayuk, E., Abdikadyrova, A., & Abugali, G. (2025). Characterizing the Genetic Basis of Winter Wheat Rust Resistance in Southern Kazakhstan. Plants, 14(7), 1146. https://doi.org/10.3390/plants14071146

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