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Volume 14
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10.3390/plants14152330
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Article

Establishment of Singleplex and Duplex TaqMan RT-qPCR Detection Systems for Strawberry Mottle Virus (SMoV) and Strawberry Vein Banding Virus (SVBV)

by
Tengfei Xu
1,2,3,†,
Dehang Gao
3,†,
Mengmeng Wu
3,
Hongqing Wang
3 and
Chengyong He
1,2,*
1
Institute of Horticulture, Sichuan Academy of Agricultural Sciences, Chengdu 610000, China
2
Key Laboratory of Horticultural Crops Biology and Germplasm Enhancement in Southwest, Ministry of Agriculture and Rural Affairs, Chengdu 610000, China
3
Department of Fruit Science, College of Horticulture, China Agricultural University, Beijing 100107, China
*
Author to whom correspondence should be addressed.
†
These authors contributed equally to this work.
Plants 2025, 14(15), 2330; https://doi.org/10.3390/plants14152330 (registering DOI)
Submission received: 20 May 2025 / Revised: 18 July 2025 / Accepted: 25 July 2025 / Published: 27 July 2025
(This article belongs to the Section Plant Molecular Biology)
Versions Notes

Abstract

SMoV and SVBV are two major viruses that pose significant threats to the global strawberry industry. Both are latent viruses, making early detection difficult due to their uneven distribution and low concentration in host tissues. Traditional RT-PCR techniques are insufficient for precise and quantitative detection. In this study, TaqMan RT-qPCR detection systems for SMoV and SVBV were established for application in practical production settings, enabling accurate, rapid, and efficient detection of strawberry viruses. When viral accumulation in plants is low, the highly sensitive TaqMan RT-qPCR technique allows for accurate quantification, facilitating the early identification of infected plants and preventing large-scale outbreaks in cultivation areas. The development of a duplex TaqMan RT-qPCR assay enables simultaneous quantification of SMoV and SVBV in a single reaction, improving detection efficiency and providing technical support for risk assessment and effective control of strawberry viral diseases.
Keywords: SMoV; SVBV; detection system; TaqMan RT-qPCR SMoV; SVBV; detection system; TaqMan RT-qPCR

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MDPI and ACS Style

Xu, T.; Gao, D.; Wu, M.; Wang, H.; He, C. Establishment of Singleplex and Duplex TaqMan RT-qPCR Detection Systems for Strawberry Mottle Virus (SMoV) and Strawberry Vein Banding Virus (SVBV). Plants 2025, 14, 2330. https://doi.org/10.3390/plants14152330

AMA Style

Xu T, Gao D, Wu M, Wang H, He C. Establishment of Singleplex and Duplex TaqMan RT-qPCR Detection Systems for Strawberry Mottle Virus (SMoV) and Strawberry Vein Banding Virus (SVBV). Plants. 2025; 14(15):2330. https://doi.org/10.3390/plants14152330

Chicago/Turabian Style

Xu, Tengfei, Dehang Gao, Mengmeng Wu, Hongqing Wang, and Chengyong He. 2025. "Establishment of Singleplex and Duplex TaqMan RT-qPCR Detection Systems for Strawberry Mottle Virus (SMoV) and Strawberry Vein Banding Virus (SVBV)" Plants 14, no. 15: 2330. https://doi.org/10.3390/plants14152330

APA Style

Xu, T., Gao, D., Wu, M., Wang, H., & He, C. (2025). Establishment of Singleplex and Duplex TaqMan RT-qPCR Detection Systems for Strawberry Mottle Virus (SMoV) and Strawberry Vein Banding Virus (SVBV). Plants, 14(15), 2330. https://doi.org/10.3390/plants14152330

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