Temporal Changes in Biochemical Responses to Salt Stress in Three Salicornia Species
Abstract
:1. Introduction
2. Results
2.1. Growth Parameters
2.2. Photosynthetic Pigments Contents
2.3. Cation Accumulation
2.4. Shoot Osmolytes Contents
2.5. Oxidative Stress Markers
2.6. Antioxidant Enzymes Activities
2.7. Correlation Analysis
2.8. Principal Component Analysis
2.9. Hierarchical Clustering
3. Discussion
4. Materials and Methods
4.1. Plant Material
4.2. Growth Parameters
4.3. Photosynthetic Pigments
4.4. Quantification of Cations
4.5. Proline and Glycine Betaine
4.6. MDA and H2O2
4.7. Antioxidant Enzyme Assays
4.8. Statistical Analyses
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Conflicts of Interest
References
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Mean Squares | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|
Df | FW | WC | Chl. a | Chl. b | Car | Na+ | K+ | Ca2+ | Mg2+ | |
Species | 2 | 0.88 ** | 17.83 ** | 1.26 ** | 0.87 ** | 0.24 ** | 218,549 ns | 10,357 ns | 211 ns | 19,505 ** |
Salinity | 2 | 0.40 ** | 135.49 ** | 20.89 ** | 8.13 ** | 2.28 ** | 935,837,253 ** | 996,868 ** | 4293 ** | 787,916 ** |
Time | 2 | 0.79 ** | 14.86 ** | 0.02 ns | 0.31 ** | 0.16 ** | 4,431,883 ** | 40,298 ** | 70 ns | 46,001 ** |
Species × Salinity | 4 | 0.05 * | 2.72 * | 0.10 ns | 0.19 ** | 0.03 ns | 857,253 ns | 15,407 * | 393 ** | 3842 ** |
Species × Time | 4 | 0.07 ** | 2.98 * | 0.10 ns | 0.06 ns | 0.01 ns | 882,716 ns | 29,642 ** | 331 * | 777 ns |
Salinity × Time | 4 | 0.10 ** | 14.64 ** | 0.49 ** | 0.42 ** | 0.19 ** | 4,675,309 ** | 4098 ns | 1096 ** | 16,638 ** |
Species × Salinity × Time | 8 | 0.02 * | 0.62 ns | 0.03 ns | 0.04 ns | 0.02 * | 1,188,642 ** | 15,534 ** | 257 * | 1948 ** |
Residuals | 54 | 0.008 | 1.11 | 0.07 | 0.05 | 0.01 | 392,747 | 4352 | 98 | 732 |
CV% | 4.11 | 1.33 | 10.48 | 10.50 | 9.87 | 8.51 | 6.05 | 1.38 | 5.36 |
Mean Squares | |||||||||
---|---|---|---|---|---|---|---|---|---|
df | PRO | GB | MDA | H2O2 | SOD | CAT | POD | APX | |
Species | 2 | 0.46 ** | 6304 ** | 0.65 ** | 0.06 * | 25.94 ** | 1.88 ** | 338.41 ** | 1.04 ** |
Salinity | 2 | 12.16 ** | 77,777 ** | 21.30 ** | 1.64 ** | 6828.96 ** | 114.59 ** | 14,595 ** | 18.60 ** |
Time | 2 | 3.88 ** | 1527 ** | 2.70 ** | 0.11 ** | 65.91 ** | 3.31 ** | 366.75 ** | 2.99 ** |
Species × Salinity | 4 | 0.19 ** | 935 ** | 0.10 ** | 0.04 * | 65.55 ** | 1.36 ** | 22.73 * | 0.13 ** |
Species × Time | 4 | 0.47 ** | 524 ** | 0.40 ** | 0.009 ns | 6.81 * | 0.28 ns | 23.84 * | 0.03 ns |
Salinity × Time | 4 | 1.27 ** | 1133 ** | 0.99 ** | 0.63 ** | 19.79 ** | 0.65 ** | 110.32 ** | 0.73 ** |
Species × Salinity × Time | 8 | 0.21 ** | 361 * | 0.18 ** | 0.02 * | 4.40 ns | 0.37 ** | 15.49 * | 0.02 * |
Residuals | 54 | 0.01 | 139 | 0.02 | 0.01 | 2.16 | 0.13 | 7.37 | 0.01 |
CV% | 9.33 | 6.22 | 9.39 | 6.89 | 5.37 | 6.24 | 5.56 | 4.43 |
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Homayouni, H.; Razi, H.; Izadi, M.; Alemzadeh, A.; Kazemeini, S.A.; Niazi, A.; Vicente, O. Temporal Changes in Biochemical Responses to Salt Stress in Three Salicornia Species. Plants 2024, 13, 979. https://doi.org/10.3390/plants13070979
Homayouni H, Razi H, Izadi M, Alemzadeh A, Kazemeini SA, Niazi A, Vicente O. Temporal Changes in Biochemical Responses to Salt Stress in Three Salicornia Species. Plants. 2024; 13(7):979. https://doi.org/10.3390/plants13070979
Chicago/Turabian StyleHomayouni, Hengameh, Hooman Razi, Mahmoud Izadi, Abbas Alemzadeh, Seyed Abdolreza Kazemeini, Ali Niazi, and Oscar Vicente. 2024. "Temporal Changes in Biochemical Responses to Salt Stress in Three Salicornia Species" Plants 13, no. 7: 979. https://doi.org/10.3390/plants13070979