Two Arabidopsis Splicing Factors, U2AF65a and U2AF65b, Differentially Control Flowering Time by Modulating the Expression or Alternative Splicing of a Subset of FLC Upstream Regulators
Abstract
:1. Introduction
2. Results
2.1. RNA Sequencing Analysis in the Shoot Apex-Enriched Samples of atu2af65a and atu2af65b Mutants
2.2. Downregulation of SOC1 and AGL24 Expression in the Shoot Apices of the atu2af65a Mutants Is Due to Upregulation of FLC Expression
2.3. COOLAIR Is Involved in Misregulation of FLC Expression in the Shoot Apices of the atu2af65a Mutants
2.4. EDM2 and PP2A-b’ɤ Genes Are Involved in Change in FLC Expression in the atu2af65a or atu2af65b Mutants
2.5. Global-Scale Changes in Splicing Events in the atu2af65a and atu2af65b Mutants
2.6. Alternative Splicing of AtU2AF65a Pre-mRNA could Be Altered Similarly by Mutations in AtU2AF65b and AtSF1 Genes as Well as Lower Ambient Growth Temperature
3. Discussion
4. Materials and Methods
4.1. Plant Material and Growth Conditions
4.2. RNA Extraction, RT-PCR and Real-Time Quantitative PCR
4.3. Preparation of Shoot Apices and RNA-seq
4.4. Nuclear/Cytoplasmic Fractionation and Isolation of Nuclear Extracts
4.5. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Lee, H.T.; Park, H.-Y.; Lee, K.C.; Lee, J.H.; Kim, J.-K. Two Arabidopsis Splicing Factors, U2AF65a and U2AF65b, Differentially Control Flowering Time by Modulating the Expression or Alternative Splicing of a Subset of FLC Upstream Regulators. Plants 2023, 12, 1655. https://doi.org/10.3390/plants12081655
Lee HT, Park H-Y, Lee KC, Lee JH, Kim J-K. Two Arabidopsis Splicing Factors, U2AF65a and U2AF65b, Differentially Control Flowering Time by Modulating the Expression or Alternative Splicing of a Subset of FLC Upstream Regulators. Plants. 2023; 12(8):1655. https://doi.org/10.3390/plants12081655
Chicago/Turabian StyleLee, Hee Tae, Hyo-Young Park, Keh Chien Lee, Jeong Hwan Lee, and Jeong-Kook Kim. 2023. "Two Arabidopsis Splicing Factors, U2AF65a and U2AF65b, Differentially Control Flowering Time by Modulating the Expression or Alternative Splicing of a Subset of FLC Upstream Regulators" Plants 12, no. 8: 1655. https://doi.org/10.3390/plants12081655