Genome-Wide Analysis of the bHLH Gene Family in Loropetalum chinense var. rubrum: Identification, Classification, Evolution, and Diversity of Expression Patterns under Cultivation
Abstract
:1. Introduction
2. Results
2.1. Identification of the LcbHLHs and Evolutionary Analysis
2.2. Multiple Sequence Alignment and Analyses of the Motifs, Domains, and Structure
2.3. Analysis of Gene Duplication and Collinear Correlations
2.4. GO Annotation and Analyses of the Cis-Regulatory Elements
2.5. Expression Profiles of LcbHLHs and qRT–PCR Analysis of Gene Expression
2.6. The Protein–Protein Interaction Network of the Candidate Genes
2.7. Overexpression of LcbHLH156
3. Discussion
3.1. Systematic and Comprehensive Genome-Wide Detection of LcbHLHs in L. chinense var. rubrum
3.2. Functional Prediction of LcbHLHs
4. Materials and Methods
4.1. Plant Materials and Data Resources
4.2. Identification and Physicochemical Characterization of the bHLH Gene Family
4.3. Chromosomal Localization, Tandem Duplication, and Collinearity of the bHLH Genes
4.4. Evolution and Multiple Sequence Alignment of LcbHLHs
4.5. Analysis of the Gene Structure, Conserved Motifs, and Family Structural Domains
4.6. GO Annotation, Analysis of the Cis-Acting Components, and the Protein–Protein Interactions of LcbHLHs
4.7. Gene Expression Patterns and Quantitative Real-Time PCR
4.8. Transient Expression of L. chinense var. rubrum and Color Measurement
4.9. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Subfamilies | Number of LcbHLHs | Number of AtbHLHs | Number of FcbHLHs | Number of OsbHLHs |
---|---|---|---|---|
Ia | 12 | 10 | 8 | 15 |
Ib | 8 | 13 | 7 | 7 |
II | 8 | 4 | 0 | 2 |
III(a + b + c) | 19 | 10 | 9 | 10 |
III(d + e) | 11 | 8 | 6 | 5 |
IIIf | 3 | 4 | 1 | 7 |
IVa | 11 | 4 | 4 | 6 |
IVb | 3 | 3 | 4 | 4 |
IVc | 7 | 4 | 3 | 4 |
IVd | 1 | 2 | 1 | 9 |
Va | 3 | 3 | 3 | 3 |
Vb | 8 | 5 | 6 | 9 |
VI | 1 | 2 | 2 | 0 |
VII(a + b) | 17 | 15 | 7 | 14 |
VIIIa | 6 | 4 | 3 | 1 |
VIII(b + c) | 11 | 11 | 14 | 18 |
IX | 8 | 6 | 5 | 5 |
X | 1 | 10 | 14 | 14 |
XI | 5 | 5 | 4 | 7 |
XII | 20 | 17 | 13 | 18 |
XIII | 0 | 3 | 4 | 3 |
XIV | 0 | 3 | 0 | 3 |
XV | 0 | 4 | 0 | 6 |
Orphans | 2 | 9 | 0 | 3 |
Motif | Sequence |
---|---|
1 | SHSLAERRRRERJNERFKALRSLVPNCSK |
2 | MDKASMLDEAIEYVKELQRQVQELSMKLE |
3 | EEPKSDYIHVRARRGQATD |
4 | QVMSFEQSNWDASVHEIQGMTSFEHPHNQDQQLHLLHEMQQNGHHHPQSF |
5 | FVQKPANFQTSLGFLGDLPTPDNASASSVLYDPLFHLNLPPQPPLFRDLF |
6 | YNLPASRTASLFGGGIDEKEGSGGVYQNGVATQFDNGVLEFTGDIGGMGK |
7 | RJVSALEKLGLDIVHANVSTF |
8 | ERAKLAKSAGIRTLVCIPTASGVVELGSTEIIKEDLGLIQLIKSLF |
9 | NSSTLPDTSPYINNPPTQHLLNLFHLPRCSPSSNLLPNSSI |
10 | DNIRLSMEELSYHQNPHQEDDAALEQHLGFDMENCYNINNN |
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Liu, Y.; Lin, L.; Liu, Y.; Mo, Q.; Zhang, D.; Li, W.; Xiong, X.; Yu, X.; Li, Y. Genome-Wide Analysis of the bHLH Gene Family in Loropetalum chinense var. rubrum: Identification, Classification, Evolution, and Diversity of Expression Patterns under Cultivation. Plants 2023, 12, 3392. https://doi.org/10.3390/plants12193392
Liu Y, Lin L, Liu Y, Mo Q, Zhang D, Li W, Xiong X, Yu X, Li Y. Genome-Wide Analysis of the bHLH Gene Family in Loropetalum chinense var. rubrum: Identification, Classification, Evolution, and Diversity of Expression Patterns under Cultivation. Plants. 2023; 12(19):3392. https://doi.org/10.3390/plants12193392
Chicago/Turabian StyleLiu, Yang, Ling Lin, Yang Liu, Qiong Mo, Damao Zhang, Weidong Li, Xingyao Xiong, Xiaoying Yu, and Yanlin Li. 2023. "Genome-Wide Analysis of the bHLH Gene Family in Loropetalum chinense var. rubrum: Identification, Classification, Evolution, and Diversity of Expression Patterns under Cultivation" Plants 12, no. 19: 3392. https://doi.org/10.3390/plants12193392