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Article

Development of Microscopic Techniques for the Visualization of Plant–Root-Knot Nematode Interaction

Department of Agri-Food Engineering and Biotechnology, Universitat Politècnica de Catalunya, Esteve Terradas 8, 08860 Castelldefels, Spain
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Academic Editor: Raymond Wightman
Plants 2022, 11(9), 1165; https://doi.org/10.3390/plants11091165
Received: 30 March 2022 / Revised: 20 April 2022 / Accepted: 22 April 2022 / Published: 26 April 2022
(This article belongs to the Special Issue Imaging Tools for the Plant Sciences)
Plant-parasitic nematodes are a significant cause of yield losses and food security issues. Specifically, nematodes of the genus Meloidogyne can cause significant production losses in horticultural crops around the world. Understanding the mechanisms of the ever-changing physiology of plant roots by imaging the galls induced by nematodes could provide a great insight into their control. However, infected roots are unsuitable for light microscopy investigation due to the opacity of plant tissues. Thus, samples must be cleared to visualize the interior of whole plants in order to make them transparent using clearing agents. This work aims to identify which clearing protocol and microscopy system is the most appropriate to obtain 3D images of tomato cv. Durinta and eggplant cv. Cristal samples infected with Meloidogyne incognita to visualize and study the root–nematode interaction. To that extent, two clearing solutions (BABB and ECi), combined with three different dehydration solvents (ethanol, methanol and 1-propanol), are tested. In addition, the advantages and disadvantages of alternative imaging techniques to confocal microscopy are analyzed by employing an experimental custom-made setup that combines two microscopic techniques, light sheet fluorescence microscopy and optical projection tomography, on a single instrument. View Full-Text
Keywords: plant 3D imaging; root-knot nematode; tissue clearing; light sheet fluorescence microscopy; optical projection tomography plant 3D imaging; root-knot nematode; tissue clearing; light sheet fluorescence microscopy; optical projection tomography
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MDPI and ACS Style

Vernet, H.; Fullana, A.M.; Sorribas, F.J.; Gualda, E.J. Development of Microscopic Techniques for the Visualization of Plant–Root-Knot Nematode Interaction. Plants 2022, 11, 1165. https://doi.org/10.3390/plants11091165

AMA Style

Vernet H, Fullana AM, Sorribas FJ, Gualda EJ. Development of Microscopic Techniques for the Visualization of Plant–Root-Knot Nematode Interaction. Plants. 2022; 11(9):1165. https://doi.org/10.3390/plants11091165

Chicago/Turabian Style

Vernet, Helena, Aïda Magdalena Fullana, Francisco Javier Sorribas, and Emilio J. Gualda. 2022. "Development of Microscopic Techniques for the Visualization of Plant–Root-Knot Nematode Interaction" Plants 11, no. 9: 1165. https://doi.org/10.3390/plants11091165

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