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Review

Advances in the Molecular Cytogenetics of Bananas, Family Musaceae

1
Institute of Experimental Botany of the Czech Academy of Sciences, Centre of the Region Hana for Biotechnological and Agricultural Research, 77900 Olomouc, Czech Republic
2
Department of Cell Biology and Genetics, Faculty of Science, Palacký University, 77900 Olomouc, Czech Republic
*
Author to whom correspondence should be addressed.
Academic Editors: Bożena Kolano and Natalia Borowska-Zuchowska
Plants 2022, 11(4), 482; https://doi.org/10.3390/plants11040482
Received: 23 December 2021 / Revised: 2 February 2022 / Accepted: 7 February 2022 / Published: 11 February 2022
(This article belongs to the Special Issue Plant Evolutionary Cytogenetics)
The banana is a staple food crop and represents an important trade commodity for millions of people living in tropical and subtropical countries. The most important edible banana clones originated from natural crosses between diploid Musa balbisiana and various subspecies of M. acuminata. It is worth mentioning that evolution and speciation in the Musaceae family were accompanied by large-scale chromosome structural changes, indicating possible reasons for lower fertility or complete sterility of these vegetatively propagated clones. Chromosomal changes, often accompanied by changes in genome size, are one of the driving forces underlying speciation in plants. They can clarify the genomic constitution of edible bananas and shed light on their origin and on diversification processes in members of the Musaceae family. This article reviews the development of molecular cytogenetic approaches, ranging from classical fluorescence in situ hybridization (FISH) using common cytogenetic markers to oligo painting FISH. We discuss differences in genome size and chromosome number across the Musaceae family in addition to the development of new chromosome-specific cytogenetic probes and their use in genome structure and comparative karyotype analysis. The impact of these methodological advances on our knowledge of Musa genome evolution at the chromosomal level is demonstrated. In addition to citing published results, we include our own new unpublished results and outline future applications of molecular cytogenetics in banana research. View Full-Text
Keywords: flow cytometry; chromosomes; fluorescence in situ hybridization; rRNA genes; DNA repeats; BAC clones; oligo painting; karyotyping flow cytometry; chromosomes; fluorescence in situ hybridization; rRNA genes; DNA repeats; BAC clones; oligo painting; karyotyping
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MDPI and ACS Style

Šimoníková, D.; Čížková, J.; Zoulová, V.; Christelová, P.; Hřibová, E. Advances in the Molecular Cytogenetics of Bananas, Family Musaceae. Plants 2022, 11, 482. https://doi.org/10.3390/plants11040482

AMA Style

Šimoníková D, Čížková J, Zoulová V, Christelová P, Hřibová E. Advances in the Molecular Cytogenetics of Bananas, Family Musaceae. Plants. 2022; 11(4):482. https://doi.org/10.3390/plants11040482

Chicago/Turabian Style

Šimoníková, Denisa, Jana Čížková, Veronika Zoulová, Pavla Christelová, and Eva Hřibová. 2022. "Advances in the Molecular Cytogenetics of Bananas, Family Musaceae" Plants 11, no. 4: 482. https://doi.org/10.3390/plants11040482

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