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Article

Optimization of Deep Eutectic Solvent Extraction of Phenolic Acids and Tannins from Alchemilla vulgaris L.

1
Faculty of Food Technology Osijek, Josip Juraj Strossmayer University of Osijek, Franje Kuhača 18, 31000 Osijek, Croatia
2
Faculty of Chemistry and Technology, University of Split, Ruđera Boškovića 35, 21000 Split, Croatia
*
Authors to whom correspondence should be addressed.
Academic Editor: Stefania Lamponi
Plants 2022, 11(4), 474; https://doi.org/10.3390/plants11040474
Received: 20 December 2021 / Revised: 2 February 2022 / Accepted: 8 February 2022 / Published: 9 February 2022
(This article belongs to the Special Issue Structural and Functional Analysis of Extracts in Plants II)
Alchemilla vulgaris L. is a good source of antioxidant components with an emphasis on phenolic acids and tannins. In this study, gallic acid, ellagic acid, and hydrolyzable tannins (HT) were extracted from this plant with different deep eutectic solvents (DESs), varying the amount of added H2O, temperature and extraction time. Seventeen DESs (n = 3) were used for the extraction, of which choline chloride:urea (1:2) proved to be the most suitable. The selection of the best solvent was followed by the examination of the influence of the extraction type and parameters using response surface methodology (RSM). Gallic acid content was in the range of 0.00–1.89 µg mg−1, ellagic acid content was 0.00–12.76 µg mg−1 and hydrolyzable tannin (HT) content was 3.06–181.26 µgTAE mg−1, depending on the used technique and the extraction conditions. According to the results, extraction by stirring and heating was the most suitable since the highest amounts of gallic acid, ellagic acid, and HT were extracted, and the obtained optimal values using response surface methodology (RSM) are confirmed by experimentally obtained values. View Full-Text
Keywords: Alchemilla vulgaris L.; optimization; gallic acid; ellagic acid; hydrolyzable tannins; extraction Alchemilla vulgaris L.; optimization; gallic acid; ellagic acid; hydrolyzable tannins; extraction
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MDPI and ACS Style

Kovač, M.J.; Jokić, S.; Jerković, I.; Molnar, M. Optimization of Deep Eutectic Solvent Extraction of Phenolic Acids and Tannins from Alchemilla vulgaris L. Plants 2022, 11, 474. https://doi.org/10.3390/plants11040474

AMA Style

Kovač MJ, Jokić S, Jerković I, Molnar M. Optimization of Deep Eutectic Solvent Extraction of Phenolic Acids and Tannins from Alchemilla vulgaris L. Plants. 2022; 11(4):474. https://doi.org/10.3390/plants11040474

Chicago/Turabian Style

Kovač, Martina Jakovljević, Stela Jokić, Igor Jerković, and Maja Molnar. 2022. "Optimization of Deep Eutectic Solvent Extraction of Phenolic Acids and Tannins from Alchemilla vulgaris L." Plants 11, no. 4: 474. https://doi.org/10.3390/plants11040474

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