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Volume 11
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10.3390/plants11243552
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Article

Suppressive Effects of Flavonoids on Macrophage-Associated Adipocyte Inflammation in a Differentiated Murine Preadipocyte 3T3-L1 Cells Co-Cultured with a Murine Macrophage RAW264.7 Cells

by
Dahae Lee
1,
Sukyong Hong
2,
Kiwon Jung
2,3,
Sungyoul Choi
4,* and
Ki Sung Kang
1,*
1
Department of Preventive Medicine, College of Korean Medicine, Gachon University, Seongnam 13120, Republic of Korea
2
College of Pharmacy, CHA University, Sungnam 13844, Republic of Korea
3
Oncobix Co., Ltd., Yongin-si 16950, Republic of Korea
4
Department of Neuropsychiatry, College of Korean Medicine, Gachon University, Seongnam 13120, Republic of Korea
*
Authors to whom correspondence should be addressed.
Plants 2022, 11(24), 3552; https://doi.org/10.3390/plants11243552
Submission received: 17 November 2022 / Revised: 11 December 2022 / Accepted: 13 December 2022 / Published: 16 December 2022
(This article belongs to the Special Issue Natural Resources of Medicinal and Cosmetic Plants Volume II)
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Abstract

The suppressive effects of flavonoids on macrophage-associated adipocyte inflammation in a differentiated murine preadipocyte cell line (3T3-L1) co-cultured with a murine macrophage cell line (RAW264.7) were evaluated. Extracellular lipid accumulation was investigated via Oil Red O staining. The expression levels of adipogenesis- and inflammation-associated proteins, including CCAAT/enhancer-binding protein (C/EBP)-α, inducible nitric oxide synthase (iNOS), C/EBPβ, peroxisome proliferator-activated receptor γ (PPARγ), and cyclooxygenase-2 (COX-2), were determined via Western blotting. Proinflammatory cytokines, including monocyte chemoattractant protein 1 (MCP-1) and interleukin-6 (IL-6), were assessed using enzyme-linked immunosorbent assay kits. We found that silybin, formononetin, and diosmetin inhibited lipid accumulation and production of proinflammatory cytokines in the co-cultures of 3T3-L1 and RAW264.7 cells. Moreover, they inhibited the protein expression of PPARγ, C/EBPα, COX-2, C/EBPβ, and iNOS in the co-cultures of 3T3-L1 and RAW264.7 cells. These data support that silybin, formononetin, and diosmetin inhibit macrophage-associated adipocyte inflammation and lipid accumulation.
Keywords: flavonoid; adipocytes; macrophages; adipogenesis; inflammation flavonoid; adipocytes; macrophages; adipogenesis; inflammation

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MDPI and ACS Style

Lee, D.; Hong, S.; Jung, K.; Choi, S.; Kang, K.S. Suppressive Effects of Flavonoids on Macrophage-Associated Adipocyte Inflammation in a Differentiated Murine Preadipocyte 3T3-L1 Cells Co-Cultured with a Murine Macrophage RAW264.7 Cells. Plants 2022, 11, 3552. https://doi.org/10.3390/plants11243552

AMA Style

Lee D, Hong S, Jung K, Choi S, Kang KS. Suppressive Effects of Flavonoids on Macrophage-Associated Adipocyte Inflammation in a Differentiated Murine Preadipocyte 3T3-L1 Cells Co-Cultured with a Murine Macrophage RAW264.7 Cells. Plants. 2022; 11(24):3552. https://doi.org/10.3390/plants11243552

Chicago/Turabian Style

Lee, Dahae, Sukyong Hong, Kiwon Jung, Sungyoul Choi, and Ki Sung Kang. 2022. "Suppressive Effects of Flavonoids on Macrophage-Associated Adipocyte Inflammation in a Differentiated Murine Preadipocyte 3T3-L1 Cells Co-Cultured with a Murine Macrophage RAW264.7 Cells" Plants 11, no. 24: 3552. https://doi.org/10.3390/plants11243552

APA Style

Lee, D., Hong, S., Jung, K., Choi, S., & Kang, K. S. (2022). Suppressive Effects of Flavonoids on Macrophage-Associated Adipocyte Inflammation in a Differentiated Murine Preadipocyte 3T3-L1 Cells Co-Cultured with a Murine Macrophage RAW264.7 Cells. Plants, 11(24), 3552. https://doi.org/10.3390/plants11243552

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