Exogenous Glutathione Promotes the Proliferation of Pinus koraiensis Embryonic Cells and the Synthesis of Glutathione and Ascorbic Acid
Abstract
:1. Introduction
2. Results
2.1. Effects of Exogenous GSH and BSO on EC Proliferation
2.2. Effects of Exogenous GSH and BSO on Intracellular GSH Synthesis
2.3. Effects of Exogenous GSH and BSO on Intracellular ASA Synthesis
2.4. Effects of Exogenous GSH and BSO on Enzymatic Activity
2.5. qRT-PCR Validation of Differentially Expressed Genes between GSH and BSO Treatments
3. Discussion
4. Materials and Methods
4.1. EC Induction and Proliferation Assays
4.2. Determination of Intracellular Antioxidant Content and Enzymatic Activity
4.3. qRT-PCR Validation Assay
4.4. Calculations and Statistical Analyses
5. Conclusions
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Unigenes | Forward Primer | Reverse Primer |
---|---|---|
c154707.graph_c1 (Internal reference) | CAGCTCAGCTTGAAATGA | ACGAGTACTGTGAGGAAA |
c180344.graph_c0 | CGACATGGTTCCAAACTA | GACCCTGAGAAAATTGTTG |
c187964.graph_c1 | TGGAAACCCTGTTTGTGAA | GCAATGGCTCGGTCATAT |
c188830.graph_c0 | CTCCTAGCTCTCAGGTCAT | CTTTGCGATGAGTTGTGC |
c181305.graph_c0 | CTGGTTGGTTACACGTTCC | TGTGCATGGATAGACCGAT |
c187768.graph_c0 | ATGGACATGAAGCATTGC | CGGTGATTGGTTACAACC |
c183265.graph_c0 | TTCTCAATCCGAATCTGGAA | AGGAGATGTCACCGTAGTA |
c190620.graph_c2 | GTGAAGCCGTGTCGAAGA | GGCGTTTCGTTTCACGTC |
c187045.graph_c0 | GCAAGAGCTACAGATCAA | GGACGAAATCGTATTCAAC |
c133544.graph_c0 | AGGGGATGAATTCAGCATA | CGCATTCACATACTTTCGA |
c187027.graph_c1 | TGATGAAGGCTTCCACAGA | CAAGTGCTATGCGAACCC |
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Gao, F.; Shi, Y.; Wang, R.; Tretyakova, I.N.; Nosov, A.M.; Shen, H.; Yang, L. Exogenous Glutathione Promotes the Proliferation of Pinus koraiensis Embryonic Cells and the Synthesis of Glutathione and Ascorbic Acid. Plants 2022, 11, 2586. https://doi.org/10.3390/plants11192586
Gao F, Shi Y, Wang R, Tretyakova IN, Nosov AM, Shen H, Yang L. Exogenous Glutathione Promotes the Proliferation of Pinus koraiensis Embryonic Cells and the Synthesis of Glutathione and Ascorbic Acid. Plants. 2022; 11(19):2586. https://doi.org/10.3390/plants11192586
Chicago/Turabian StyleGao, Fang, Yujie Shi, Ruirui Wang, Iraida Nikolaevna Tretyakova, Alexander Mikhaylovich Nosov, Hailong Shen, and Ling Yang. 2022. "Exogenous Glutathione Promotes the Proliferation of Pinus koraiensis Embryonic Cells and the Synthesis of Glutathione and Ascorbic Acid" Plants 11, no. 19: 2586. https://doi.org/10.3390/plants11192586