Co-Inoculation with Azospirillum brasilense and Bradyrhizobium sp. Enhances Nitrogen Uptake and Yield in Field-Grown Cowpea and Did Not Change N-Fertilizer Recovery
Abstract
:1. Introduction
2. Results
2.1. Soil and Root Nitrogen Fractions
2.2. Leaf Nitrogen Fractions and Chlorophyll Pigment
2.3. Biomass Production and Grain Yield
2.4. Shoot and Root Nitrogen Accumulation and 15N-Fertilizer Recovery
3. Discussion
4. Materials and Methods
4.1. Field-Grown Site Characterization
4.2. Experimental Design and Treatments
4.3. Cowpea Crop Management
4.4. Samplings, Measurements and Analysis
4.4.1. Leaf, Root, Nodule and Soil Collection
4.4.2. Nitrogen Fractions in Plant Tissue and Soluble Protein Analysis
4.4.3. Chlorophyll Pigment Analysis
4.4.4. Shoot Biomass and Cowpea Grain Yield
4.4.5. Total N Accumulation and 15N-Fertilizer Recovery in Cowpea Shoot and Grain
4.5. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Soil Chemical Attributes | 0–0.20 m Layer |
---|---|
Total N | 1.04 g kg−1 |
P (resin) | 39.0 mg kg−1 |
S (SO4) | 30.0 mg kg−1 |
Organic matter | 21.0 g kg−1 |
pH (CaCl2) | 5.1 |
K | 2.3 mmolc kg−1 |
Ca | 31.0 mmolc kg−1 |
Mg | 33.0 mmolc kg−1 |
H + Al | 34.0 mmolc kg−1 |
Al | 0.0 mmolc kg−1 |
B (hot water) | 0.23 mg kg−1 |
Cu (DTPA) | 3.7 mg kg−1 |
Fe (DTPA) | 25.0 mg kg−1 |
Mn (DTPA) | 30.1 mg kg−1 |
Zn (DTPA) | 1.7 mg kg−1 |
Cation exchange capacity (pH 7.0) | 100.3 mmolc kg−1 |
Base saturation (%) | 66 |
Bradyrhizobia populations in soil | 3.5 × 104 cells g−1 soil |
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Galindo, F.S.; Pagliari, P.H.; da Silva, E.C.; Silva, V.M.; Fernandes, G.C.; Rodrigues, W.L.; Céu, E.G.O.; de Lima, B.H.; Jalal, A.; Muraoka, T.; et al. Co-Inoculation with Azospirillum brasilense and Bradyrhizobium sp. Enhances Nitrogen Uptake and Yield in Field-Grown Cowpea and Did Not Change N-Fertilizer Recovery. Plants 2022, 11, 1847. https://doi.org/10.3390/plants11141847
Galindo FS, Pagliari PH, da Silva EC, Silva VM, Fernandes GC, Rodrigues WL, Céu EGO, de Lima BH, Jalal A, Muraoka T, et al. Co-Inoculation with Azospirillum brasilense and Bradyrhizobium sp. Enhances Nitrogen Uptake and Yield in Field-Grown Cowpea and Did Not Change N-Fertilizer Recovery. Plants. 2022; 11(14):1847. https://doi.org/10.3390/plants11141847
Chicago/Turabian StyleGalindo, Fernando Shintate, Paulo Humberto Pagliari, Edson Cabral da Silva, Vinicius Martins Silva, Guilherme Carlos Fernandes, Willian Lima Rodrigues, Elaine Garcia Oliveira Céu, Bruno Horschut de Lima, Arshad Jalal, Takashi Muraoka, and et al. 2022. "Co-Inoculation with Azospirillum brasilense and Bradyrhizobium sp. Enhances Nitrogen Uptake and Yield in Field-Grown Cowpea and Did Not Change N-Fertilizer Recovery" Plants 11, no. 14: 1847. https://doi.org/10.3390/plants11141847