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Article

Brevilin A Isolated from Centipeda minima Induces Apoptosis in Human Gastric Cancer Cells via an Extrinsic Apoptotic Signaling Pathway

1
College of Korean Medicine, Gachon University, Seongnam 13120, Korea
2
Yonsei Institute of Pharmaceutical Sciences, College of Pharmacy, Yonsei University, Incheon 21983, Korea
3
D. Nature Co., Ltd., Seongnam 13174, Korea
4
College of Pharmacy, Wonkwang University, Iksan 54538, Korea
5
Department of Food Science and Nutrition, Gyeongsang National University, Jinju 52725, Korea
*
Authors to whom correspondence should be addressed.
These authors contributed equally to this work.
Academic Editor: Itay Gonda
Plants 2022, 11(13), 1658; https://doi.org/10.3390/plants11131658
Received: 24 May 2022 / Revised: 19 June 2022 / Accepted: 20 June 2022 / Published: 23 June 2022
Brevilin A, which has anticancer activities against a range of cancers, is an abundant constituent of the medicinal herb Centipeda minima (L.) A. Braun & Asch, which has also been reported to have anticancer activity against breast cancer cells. However, the anticancer activities of C. minima and brevilin A against human gastric cancer have yet to be reported. In this study, we aimed to evaluate the cytotoxicity and molecular basis underlying the anticancer activities of extracts of C. minima (CMX) and brevilin A against human gastric cancer (AGS) cells. We deduced the potential targets and mechanisms underlying the anticancer activity of brevilin A based on a network pharmacology approach. CCND1, CDK4, and BCL2L1 were identified as the key anticancer genes targeted by brevilin A. Cytotoxicity analyses revealed that CMX and brevilin A reduced the viability of AGS cells to levels below 50% (9.73 ± 1.29 µg/mL and 54.69 ± 1.38 μM, respectively). Furthermore, Hoechst 33342, annexin V, and propidium iodide staining and western blot analyses revealed that CMX and brevilin A promoted a significant induction of apoptotic cell death by upregulating the expression of cleaved caspase-8 and cleaved caspase-3 and reducing the ratio of Bax to Bcl-2, which is partially consistent with the findings of our network pharmacology analysis. Collectively, our observations indicate that CMX and brevilin A are novel sources of herbal medicine with potential utility as effective agents for the treatment of gastric cancer. View Full-Text
Keywords: Centipeda minima; brevilin A; network pharmacology; caspase-8; extrinsic apoptosis Centipeda minima; brevilin A; network pharmacology; caspase-8; extrinsic apoptosis
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MDPI and ACS Style

Lee, D.; Kwak, H.J.; Kim, B.H.; Kim, D.-W.; Kim, H.Y.; Kim, S.H.; Kang, K.S. Brevilin A Isolated from Centipeda minima Induces Apoptosis in Human Gastric Cancer Cells via an Extrinsic Apoptotic Signaling Pathway. Plants 2022, 11, 1658. https://doi.org/10.3390/plants11131658

AMA Style

Lee D, Kwak HJ, Kim BH, Kim D-W, Kim HY, Kim SH, Kang KS. Brevilin A Isolated from Centipeda minima Induces Apoptosis in Human Gastric Cancer Cells via an Extrinsic Apoptotic Signaling Pathway. Plants. 2022; 11(13):1658. https://doi.org/10.3390/plants11131658

Chicago/Turabian Style

Lee, Dahae, Hee J. Kwak, Byoung H. Kim, Dong-Wook Kim, Hyun Y. Kim, Seung H. Kim, and Ki S. Kang. 2022. "Brevilin A Isolated from Centipeda minima Induces Apoptosis in Human Gastric Cancer Cells via an Extrinsic Apoptotic Signaling Pathway" Plants 11, no. 13: 1658. https://doi.org/10.3390/plants11131658

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