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Effects on Capsicum annuum Plants Colonized with Trichoderma atroviride P. Karst Strains Genetically Modified in Taswo1, a Gene Coding for a Protein with Expansin-like Activity

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Centro de Investigación en Biotecnología, Universidad Autónoma del Estado de Morelos, Cuernavaca 62209, Morelos, Mexico
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Laboratorio Nacional de Genómica para la Biodiversidad, Cinvestav Campus Guanajuato, Km. 9.6 Libramiento Norte, Carretera Irapuato León, Irapuato 36824, Gto, Mexico
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Facultad de Ciencias Biológicas, Universidad Autónoma del Estado de Morelos Cuernavaca, Cuernavaca 62209, Morelos, Mexico
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Cátedras CONACyT-Instituto de Biotecnología, Universidad Nacional Autónoma de México, Cuernavaca 62209, Morelos, Mexico
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Centro de Investigación en Dinámica Celular, Instituto de Investigación en Ciencias Básicas y Aplicadas, Universidad Autónoma del Estado de Morelos, Cuernavaca 62209, Morelos, Mexico
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Instituto de Biotecnología, Universidad Nacional Autónoma de México, Cuernavaca 62209, Morelos, Mexico
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Departamento de Ingeniería Bioquímica, Cinvestav Irapuato, Libramiento Norte Carretera Irapuato León Kilómetro 9.6, Carretera Irapuato León, Irapuato 36821, Gto, Mexico
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Centro Universitario de Ciencias Exactas e Ingeniería, Departamento de Farmacobiología, Universidad de Guadalajara, García Barragán # 1451, Guadalajara 44430, Jalisco, Mexico
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Authors to whom correspondence should be addressed.
Academic Editor: Walter Chitarra
Plants 2021, 10(9), 1919; https://doi.org/10.3390/plants10091919
Received: 24 August 2021 / Revised: 7 September 2021 / Accepted: 13 September 2021 / Published: 15 September 2021
(This article belongs to the Topic Mechanisms of Resistance to Plant Diseases)
Here, we analyzed the effects on Capsicum annuum plants of Trichoderma atroviride P. Karst strains altered in the expression of SWOLLENIN (SWO1), a protein with amorphogenic activity on plant cell wall components. Strains of T. atroviride that overexpressed the Taswo1 gene were constructed as well as deletion mutants. A novel, cheap and accurate method for assessing root colonization was developed. Colonization assays showed that the Taswo1 overexpressing strains invaded the host root better than the WT, resulting in a stronger plant growth-promoting effect. The expression of plant defense marker genes for both the systemic acquired resistance and induced systemic resistance pathways was enhanced in plants inoculated with Taswo1 overexpressing strains, while inoculation with deletion mutant strains resulted in a similar level of expression to that observed upon inoculation with the wild-type strain. Response to pathogen infection was also enhanced in the plants inoculated with the Taswo1 overexpressing strains, and surprisingly, an intermediate level of protection was achieved with the mutant strains. Tolerance to abiotic stresses was also higher in plants inoculated with the Taswo1 overexpressing strains but was similar in plants inoculated with the wild-type or the mutant strains. Compatible osmolyte production in drought conditions was studied. This study may contribute to improving Trichoderma biocontrol and biofertilization abilities. View Full-Text
Keywords: Trichoderma; phytopathogen resistance; stress tolerance; SWOLLENIN Trichoderma; phytopathogen resistance; stress tolerance; SWOLLENIN
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MDPI and ACS Style

Sánchez-Cruz, R.; Mehta, R.; Atriztán-Hernández, K.; Martínez-Villamil, O.; del Rayo Sánchez-Carbente, M.; Sánchez-Reyes, A.; Lira-Ruan, V.; González-Chávez, C.A.; Tabche-Barrera, M.L.; Bárcenas-Rodríguez, R.C.; Batista-García, R.A.; Herrera-Estrella, A.; Balcázar-López, E.; Folch-Mallol, J.L. Effects on Capsicum annuum Plants Colonized with Trichoderma atroviride P. Karst Strains Genetically Modified in Taswo1, a Gene Coding for a Protein with Expansin-like Activity. Plants 2021, 10, 1919. https://doi.org/10.3390/plants10091919

AMA Style

Sánchez-Cruz R, Mehta R, Atriztán-Hernández K, Martínez-Villamil O, del Rayo Sánchez-Carbente M, Sánchez-Reyes A, Lira-Ruan V, González-Chávez CA, Tabche-Barrera ML, Bárcenas-Rodríguez RC, Batista-García RA, Herrera-Estrella A, Balcázar-López E, Folch-Mallol JL. Effects on Capsicum annuum Plants Colonized with Trichoderma atroviride P. Karst Strains Genetically Modified in Taswo1, a Gene Coding for a Protein with Expansin-like Activity. Plants. 2021; 10(9):1919. https://doi.org/10.3390/plants10091919

Chicago/Turabian Style

Sánchez-Cruz, Ricardo, Richa Mehta, Karina Atriztán-Hernández, Olivia Martínez-Villamil, María del Rayo Sánchez-Carbente, Ayixon Sánchez-Reyes, Verónica Lira-Ruan, Carlos A. González-Chávez, María L. Tabche-Barrera, Roberto C. Bárcenas-Rodríguez, Ramón A. Batista-García, Alfredo Herrera-Estrella, Edgar Balcázar-López, and Jorge L. Folch-Mallol. 2021. "Effects on Capsicum annuum Plants Colonized with Trichoderma atroviride P. Karst Strains Genetically Modified in Taswo1, a Gene Coding for a Protein with Expansin-like Activity" Plants 10, no. 9: 1919. https://doi.org/10.3390/plants10091919

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