Next Article in Journal
Chemical Composition, Production of Secondary Metabolites and Antioxidant Activity in Coffee Cultivars Susceptible and Partially Resistant to Bacterial Halo Blight
Next Article in Special Issue
Genetic Diversity of Rhanterium eppaposum Oliv. Populations in Kuwait as Revealed by GBS
Previous Article in Journal
Germination and the Initial Seedling Growth of Lettuce, Celeriac and Wheat Cultivars after Micronutrient and a Biological Application Pre-Sowing Seed Treatment
Previous Article in Special Issue
Genetic Diversity of Phenotypic and Biochemical Traits in VIR Radish (Raphanus sativus L.) Germplasm Collection
 
 
Review

RNA Interference and CRISPR/Cas Gene Editing for Crop Improvement: Paradigm Shift towards Sustainable Agriculture

1
Department of Microbiology, School of Life Sciences, Central University of Rajasthan, Ajmer 305801, Rajasthan, India
2
Centre for Energy and Environment, Malaviya National Institute of Technology, Jaipur 302017, Rajasthan, India
3
Department of Plant Breeding, Swedish University of Agricultural Sciences, P.O. Box 101, 230 53 Alnarp, Sweden
*
Authors to whom correspondence should be addressed.
Academic Editor: Baohong Zhang
Plants 2021, 10(9), 1914; https://doi.org/10.3390/plants10091914
Received: 30 June 2021 / Revised: 25 August 2021 / Accepted: 26 August 2021 / Published: 14 September 2021
(This article belongs to the Special Issue Genetic Diversity and Molecular Breeding in Plants)
With the rapid population growth, there is an urgent need for innovative crop improvement approaches to meet the increasing demand for food. Classical crop improvement approaches involve, however, a backbreaking process that cannot equipoise with increasing crop demand. RNA-based approaches i.e., RNAi-mediated gene regulation and the site-specific nuclease-based CRISPR/Cas9 system for gene editing has made advances in the efficient targeted modification in many crops for the higher yield and resistance to diseases and different stresses. In functional genomics, RNA interference (RNAi) is a propitious gene regulatory approach that plays a significant role in crop improvement by permitting the downregulation of gene expression by small molecules of interfering RNA without affecting the expression of other genes. Gene editing technologies viz. the clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated protein (CRISPR/Cas) have appeared prominently as a powerful tool for precise targeted modification of nearly all crops’ genome sequences to generate variation and accelerate breeding efforts. In this regard, the review highlights the diverse roles and applications of RNAi and CRISPR/Cas9 system as powerful technologies to improve agronomically important plants to enhance crop yields and increase tolerance to environmental stress (biotic or abiotic). Ultimately, these technologies can prove to be important in view of global food security and sustainable agriculture. View Full-Text
Keywords: crop; CRISPR/Cas9; resistance; RNA interference; stress crop; CRISPR/Cas9; resistance; RNA interference; stress
Show Figures

Graphical abstract

MDPI and ACS Style

Rajput, M.; Choudhary, K.; Kumar, M.; Vivekanand, V.; Chawade, A.; Ortiz, R.; Pareek, N. RNA Interference and CRISPR/Cas Gene Editing for Crop Improvement: Paradigm Shift towards Sustainable Agriculture. Plants 2021, 10, 1914. https://doi.org/10.3390/plants10091914

AMA Style

Rajput M, Choudhary K, Kumar M, Vivekanand V, Chawade A, Ortiz R, Pareek N. RNA Interference and CRISPR/Cas Gene Editing for Crop Improvement: Paradigm Shift towards Sustainable Agriculture. Plants. 2021; 10(9):1914. https://doi.org/10.3390/plants10091914

Chicago/Turabian Style

Rajput, Meenakshi, Khushboo Choudhary, Manish Kumar, V. Vivekanand, Aakash Chawade, Rodomiro Ortiz, and Nidhi Pareek. 2021. "RNA Interference and CRISPR/Cas Gene Editing for Crop Improvement: Paradigm Shift towards Sustainable Agriculture" Plants 10, no. 9: 1914. https://doi.org/10.3390/plants10091914

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop