Evaluation of the Conditions for the Cultivation of Callus Cultures of Hyssopus officinalis Regarding the Yield of Polyphenolic Compounds
Abstract
:1. Introduction
2. Results
2.1. Callus Growth Curve
2.2. The Yield of Phenolic Compounds
2.3. The Yield of Saponins
3. Discussion
4. Materials and Methods
4.1. Obtaining Plant Callus Cell Cultures
4.2. Cultivation of Callus Cell Cultures
4.3. Study of Growth Characteristics of Callus Cell Cultures (In Vitro)
4.4. Drying of Callus Cell Culture Biomass
4.5. Preparation of Extract Samples
4.6. Quantitative Determination of the Contents of Individual Phenolic Compounds by HPLC
4.7. Determination of the Total Polyphenol Compounds
4.8. Determination of Total Flavonoids
4.9. Qualitative and Quantitative Determination of Saponins
4.10. Antioxidant Activity
4.11. Statistical Analysis
5. Conclusions
Author Contributions
Funding
Data Availability Statement
Conflicts of Interest
References
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Name of Phenolic Compound | Rt (min) ± 0.5 | SD | NP | Culture Media | |||||
---|---|---|---|---|---|---|---|---|---|
MS-1 | MS-2 | MS-3 | B5-1 | B5-2 | B5-3 | ||||
Ferulic acid | 17.60 | 1.00 | 36.92 | 28.62 | 31.15 | 29.17 | 22.37 | 11.34 | 20.43 |
Isoquercitrin | 27.80 | 0.50 | 32.78 | 22.83 | 27.62 | 25.14 | 17.13 | 9.76 | 19.71 |
Rutin | 28.50 | 1.00 | 21.93 | 17.43 | 19.75 | 18.19 | 12.45 | 8.34 | 13.43 |
Quercetin | 32.00 | 0.10 | 1.79 | 0.97 | 1.14 | 1.03 | 0.54 | 0.41 | 0.50 |
Quercetin-7-O-glucoside | 35.20 | 0.10 | 0.89 | 0.45 | 0.67 | 0.57 | 0.24 | <0.2 | 0.40 |
Luteolin | 48.20 | 0.10 | 2.25 | 1.14 | 1.98 | 1.60 | 0.76 | 0.31 | 0.82 |
Components | MS-1 | MS-2 | MS-3 | B5-1 | B5-2 | B5-3 |
---|---|---|---|---|---|---|
Sucrose, g | 30 | 30 | 30 | 30 | 30 | 30 |
Casein Hydrolyzate, mg | 500 | - | - | 500 | - | - |
Myo-inositol, mg | 100 | 100 | 100 | 100 | 100 | 100 |
Thiamine, mg | 0.1 | 0.1 | 0.1 | 10.0 | 10.0 | 10.0 |
Pyridoxine, mg | 0.5 | 0.5 | 0.5 | 1.0 | 1.0 | 1.0 |
Nicotinic acid, mg | 0.5 | 0.5 | 0.5 | 1.0 | 1.0 | 1.0 |
Kinetin, mg | - | 2 | - | - | 2 | - |
6-benzylaminopurine, mg | 0.5 | - | 1.0 | 0.5 | - | 1.0 |
Indolylacetic acid, mg | - | - | 2 | - | - | 2 |
1-naphthylacetic acid, mg | - | 3 | - | - | 3 | - |
2,4-dichlorophenoxyacetic acid, mg | 2.0 | - | - | 2.0 | - | - |
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Babich, O.; Sukhikh, S.; Pungin, A.; Astahova, L.; Chupakhin, E.; Belova, D.; Prosekov, A.; Ivanova, S. Evaluation of the Conditions for the Cultivation of Callus Cultures of Hyssopus officinalis Regarding the Yield of Polyphenolic Compounds. Plants 2021, 10, 915. https://doi.org/10.3390/plants10050915
Babich O, Sukhikh S, Pungin A, Astahova L, Chupakhin E, Belova D, Prosekov A, Ivanova S. Evaluation of the Conditions for the Cultivation of Callus Cultures of Hyssopus officinalis Regarding the Yield of Polyphenolic Compounds. Plants. 2021; 10(5):915. https://doi.org/10.3390/plants10050915
Chicago/Turabian StyleBabich, Olga, Stanislav Sukhikh, Artem Pungin, Lidiya Astahova, Evgeny Chupakhin, Daria Belova, Alexander Prosekov, and Svetlana Ivanova. 2021. "Evaluation of the Conditions for the Cultivation of Callus Cultures of Hyssopus officinalis Regarding the Yield of Polyphenolic Compounds" Plants 10, no. 5: 915. https://doi.org/10.3390/plants10050915