Arsenic Toxicity-Induced Physiological and Metabolic Changes in the Shoots of Pteris cretica and Spinacia oleracea
Abstract
:1. Introduction
2. Results
2.1. Arsenic Accumulation and Translocation
2.2. Effect of Arsenic on Plant Growth
2.3. Effect of Arsenic on Nutrient Content
2.4. Effect of Arsenic on Photosynthetic Pigments
2.5. Effect of Arsenic on Leaf Gas-Exchange Parameters, Chlorophyll Fluorescence, and Water Potential
2.6. Effect of Arsenic on Free Amino Acid Metabolism
3. Discussion
4. Materials and Methods
4.1. Plant Material and Pot Experiment
4.2. Analysis of Arsenic and Elements
4.3. Analysis of Total Nitrogen
4.4. Gas-Exchange Parameter Measurements
4.5. Determination of Total Chlorophyll and Carotenoids
4.6. Chlorophyll Fluorescence Measurements
4.7. Water Potential
4.8. Analysis of Free Amino Acids
4.9. Statistical Analyses
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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P. cretica | S. oleracea | |||||
---|---|---|---|---|---|---|
TF | BF (Shoots) | BF (Roots) | TF | BF (Shoots) | BF (Roots) | |
control | 1.82 ± 0.06 a | 2.65 ± 0.02 a | 1.46 ± 0.06 a | 0.67 ± 0.02 b | 0.13 ± 0.01 b | 0.20 ± 0.01 a |
As20 | 3.28 ± 0.06 ab | 29.34 ± 0.13 ab | 8.96 ± 0.11 b | 0.26 ± 0.01 ab | 0.10 ± 0.01 ab | 0.41 ± 0.01 ab |
As100 | 5.53 ± 0.25 b | 34.96 ± 0.12 b | 6.35 ± 0.32 ab | 0.05 ± 0.01 a | 0.05 ± 0.01 a | 1.10 ± 0.01 b |
P. cretica | S. oleracea | |||||
---|---|---|---|---|---|---|
Control | As20 | As100 | Control | As20 | As100 | |
N (g/kg DW) | 26.22 ± 0.14 a | 28.54 ± 0.10 ab | 30.43 ± 0.02 b | 42.42 ± 0.29 a | 44.12 ± 0.22 ab | 47.19 ± 0.25 b |
Fe (g/kg DW) | 0.12 ± 0.01 a | 0.22 ± 0.01 b | 0.19 ± 0.01 ab | 0.25 ± 0.01 b | 0.16 ± 0.01 a | 0.21 ± 0.01 ab |
Mg (g/kg DW) | 3.44 ± 0.02 a | 3.87 ± 0.02 ab | 4.25 ± 0.03 b | 12.01 ± 0.03 b | 11.56 ± 0.01 ab | 9.06 ± 0.11 a |
S (g/kg DW) | 1.61 ± 0.01 a | 2.19 ± 0.02 ab | 2.95 ± 0.01 b | 2.28 ± 0.01 a | 2.88 ± 0.01 ab | 3.46 ± 0.01 b |
P (g/kg DW) | 2.96 ± 0.01 a | 4.15 ± 0.02 b | 3.86 ± 0.02 ab | 5.43 ± 0.02 b | 5.15 ± 0.08 ab | 3.6 ± 0.05 a |
Mn (mg/kg DW) | 56.40 ± 0.40 a | 63.68 ± 0.69 ab | 65.39 ± 0.29 b | 145.02 ± 0.54 b | 108.68 ± 0.57 ab | 106.48 ± 0.90 a |
Zn (mg/kg DW) | 24.38 ± 0.19 a | 29.00 ± 0.12 ab | 33.68 ± 0.14 b | 88.00 ± 0.55 b | 72.70 ± 0.05 ab | 48.32 ± 0.15 a |
Cu (mg/kg DW) | 8.10 ± 0.05 a | 11.04 ± 0.01 b | 10.64 ± 0.04 ab | 8.91 ± 0.04 b | 7.99 ± 0.06 ab | 7.71 ± 0.09 a |
Ni (mg/kg DW) | 0.88 ± 0.08 a | 0.88 ± 0.04 a | 1.90 ± 0.02 a | 0.94 ± 0.08 a | 0.84 ± 0.02 a | 0.60 ± 0.04 a |
P. cretica | S. oleracea | |||
---|---|---|---|---|
Chltot (g/m2) | Car (g/m2) | Chltot (g/m2) | Car (g/m2) | |
control | 0.35 ± 0.08 a | 0.05 ± 0.01 a | 0.30 ± 0.02 b | 0.05 ± 0.01 a |
As20 | 0.23 ± 0.06 a | 0.03 ± 0.01 a | 0.25 ± 0.01 ab | 0.05 ± 0.01 a |
As100 | 0.22 ± 0.04 a | 0.03 ± 0.01 a | 0.21 ± 0.03 a | 0.04 ± 0.01 a |
P. cretica | PN (μmol/m2 s) | Ci [μmol(CO2)/mol] | E [mmol(H2O)/m2s] | gs [mol(H2O)/m2s] | WUE [mol(CO2)/mol(H2O)] |
---|---|---|---|---|---|
control | 8.08 ± 0.03 c | 298.60 ± 12.15 a | 0.78 ± 0.02 b | 0.03 ± 0.001 b | 10.39 ± 0.21 ab |
As20 | 7.72 ± 0.01 b | 319.60 ± 16.59 a | 0.60 ± 0.03 a | 0.02 ± 0.002 a | 13.45 ± 0.76 b |
As100 | 7.31 ± 0.01 a | 302.13 ± 9.51 a | 0.77 ± 0.01 b | 0.03 ± 0.001 b | 9.48 ± 0.17 a |
S. oleracea | |||||
control | 11.13 ± 0.26 b | 297.25 ± 4.53 b | 2.65 ± 0.03 ab | 0.20 ± 0.004 b | 4.20 ± 0.09 b |
As20 | 10.73 ± 0.14 b | 273.39 ± 3.63 a | 2.78 ± 0.03 b | 0.18 ± 0.004 a | 3.87 ± 0.07 a |
As100 | 10.14 ± 0.18 a | 279.32 ± 1.07 ab | 2.54 ± 0.04 a | 0.18 ± 0.005 a | 4.01 ± 0.06 ab |
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Zemanová, V.; Pavlíková, D.; Hnilička, F.; Pavlík, M. Arsenic Toxicity-Induced Physiological and Metabolic Changes in the Shoots of Pteris cretica and Spinacia oleracea. Plants 2021, 10, 2009. https://doi.org/10.3390/plants10102009
Zemanová V, Pavlíková D, Hnilička F, Pavlík M. Arsenic Toxicity-Induced Physiological and Metabolic Changes in the Shoots of Pteris cretica and Spinacia oleracea. Plants. 2021; 10(10):2009. https://doi.org/10.3390/plants10102009
Chicago/Turabian StyleZemanová, Veronika, Daniela Pavlíková, František Hnilička, and Milan Pavlík. 2021. "Arsenic Toxicity-Induced Physiological and Metabolic Changes in the Shoots of Pteris cretica and Spinacia oleracea" Plants 10, no. 10: 2009. https://doi.org/10.3390/plants10102009