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Review

RNA-Based Technologies for Engineering Plant Virus Resistance

1
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences, 117997 Moscow, Russia
2
The James Hutton Institute, Invergowrie, Dundee DD2 5DA, UK
3
Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Leninskie Gory, 119991 Moscow, Russia
*
Authors to whom correspondence should be addressed.
Plants 2021, 10(1), 82; https://doi.org/10.3390/plants10010082
Received: 28 November 2020 / Revised: 25 December 2020 / Accepted: 27 December 2020 / Published: 2 January 2021
(This article belongs to the Special Issue Advances in Engineering Virus-Resistant Plants)
In recent years, non-coding RNAs (ncRNAs) have gained unprecedented attention as new and crucial players in the regulation of numerous cellular processes and disease responses. In this review, we describe how diverse ncRNAs, including both small RNAs and long ncRNAs, may be used to engineer resistance against plant viruses. We discuss how double-stranded RNAs and small RNAs, such as artificial microRNAs and trans-acting small interfering RNAs, either produced in transgenic plants or delivered exogenously to non-transgenic plants, may constitute powerful RNA interference (RNAi)-based technology that can be exploited to control plant viruses. Additionally, we describe how RNA guided CRISPR-CAS gene-editing systems have been deployed to inhibit plant virus infections, and we provide a comparative analysis of RNAi approaches and CRISPR-Cas technology. The two main strategies for engineering virus resistance are also discussed, including direct targeting of viral DNA or RNA, or inactivation of plant host susceptibility genes. We also elaborate on the challenges that need to be overcome before such technologies can be broadly exploited for crop protection against viruses. View Full-Text
Keywords: dsRNA; siRNA; miRNA; lncRNA; amiRNA; tasiRNA; RNAi; CRISPR-Cas dsRNA; siRNA; miRNA; lncRNA; amiRNA; tasiRNA; RNAi; CRISPR-Cas
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MDPI and ACS Style

Taliansky, M.; Samarskaya, V.; Zavriev, S.K.; Fesenko, I.; Kalinina, N.O.; Love, A.J. RNA-Based Technologies for Engineering Plant Virus Resistance. Plants 2021, 10, 82. https://doi.org/10.3390/plants10010082

AMA Style

Taliansky M, Samarskaya V, Zavriev SK, Fesenko I, Kalinina NO, Love AJ. RNA-Based Technologies for Engineering Plant Virus Resistance. Plants. 2021; 10(1):82. https://doi.org/10.3390/plants10010082

Chicago/Turabian Style

Taliansky, Michael, Viktoria Samarskaya, Sergey K. Zavriev, Igor Fesenko, Natalia O. Kalinina, and Andrew J. Love. 2021. "RNA-Based Technologies for Engineering Plant Virus Resistance" Plants 10, no. 1: 82. https://doi.org/10.3390/plants10010082

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