Next Article in Journal
Aortic Oxidative Stress, Inflammation and DNA Damage Following Pulmonary Exposure to Cerium Oxide Nanoparticles in a Rat Model of Vascular Injury
Previous Article in Journal
Proteomic Insights into Phycobilisome Degradation, A Selective and Tightly Controlled Process in The Fast-Growing Cyanobacterium Synechococcus elongatus UTEX 2973
Open AccessArticle

Inactivation of Aldehyde Dehydrogenase by Disulfiram in the Presence and Absence of Lipoic Acid or Dihydrolipoic Acid: An in Vitro Study

Chair of Medical Biochemistry, Jagiellonian University Medical College, 31-034 Kraków, Poland
*
Author to whom correspondence should be addressed.
Biomolecules 2019, 9(8), 375; https://doi.org/10.3390/biom9080375
Received: 27 June 2019 / Revised: 2 August 2019 / Accepted: 13 August 2019 / Published: 16 August 2019
(This article belongs to the Section Biochemistry)
The inhibition of aldehyde dehydrogenase (ALDH) by disulfiram (DSF) in vitro can be prevented and/or reversed by dithiothreitol (DTT), which is a well-known low molecular weight non-physiological redox reagent commonly used in laboratory experiments. These observations inspired us to ask the question whether the inhibition of ALDH by DSF can be preserved or abolished also by dihydrolipoic acid (DHLA), which is the only currently known low molecular weight physiological dithiol in the body of humans and other animals. It can even be metaphorized that DHLA is an “endogenous DTT”. Lipoic acid (LA) is the oxidized form of DHLA. We investigated the inactivation of ALDH derived from yeast and rat liver by DSF in the presence or absence of LA or DHLA. The results clearly show that DHLA is able both to restore and protect ALDH activity blocked by DSF. The proposed mechanism is discussed. View Full-Text
Keywords: aldehyde dehydrogenase; dihydrolipoic acid; disulfiram; dithiothreitol; lipoic acid aldehyde dehydrogenase; dihydrolipoic acid; disulfiram; dithiothreitol; lipoic acid
Show Figures

Figure 1

MDPI and ACS Style

Bilska-Wilkosz, A.; Górny, M.; Iciek, M. Inactivation of Aldehyde Dehydrogenase by Disulfiram in the Presence and Absence of Lipoic Acid or Dihydrolipoic Acid: An in Vitro Study. Biomolecules 2019, 9, 375.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop