Lnc-EPB41-Protein Interactions Associated with Congenital Pouch Colon
Sonal Gupta 1,2,†
, Nidhi Gupta 3,†, Pradeep Tiwari 1,4,†, Saji Menon 5, Praveen Mathur 6, Shanker Lal Kothari 2, Sivaramaiah Nallapeta 5, Krishna Mohan Medicherla 1,* and Prashanth Suravajhala 1,*
, Nidhi Gupta 3,†, Pradeep Tiwari 1,4,†, Saji Menon 5, Praveen Mathur 6, Shanker Lal Kothari 2, Sivaramaiah Nallapeta 5, Krishna Mohan Medicherla 1,* and Prashanth Suravajhala 1,*
1
Department of Biotechnology and Bioinformatics, Birla Institute of Scientific Research (BISR), Statue Circle, Jaipur 302021, India
2
Department of Biotechnology, Amity University Rajasthan, Kant Kalwar, Jaipur 303002, India
3
Department of Biotechnology, IIS University, Mansarovar, Jaipur 302020, India
4
Department of Chemistry, School of Basic Science, Manipal University, Jaipur 303007, India
5
NanoTemper Technologies, World Trade Centre, Bengaluru 302017, India
6
Department of Paediatric Surgery, SMS Medical College, JLN Marg, Jaipur 302004, India
*
Authors to whom correspondence should be addressed.
†
These authors contributed equally to this work.
Biomolecules 2018, 8(3), 95; https://doi.org/10.3390/biom8030095
Received: 21 August 2018 / Revised: 10 September 2018 / Accepted: 13 September 2018 / Published: 17 September 2018
(This article belongs to the Special Issue Biomolecules: Insights from Single Molecule, Single Cell, and Systems Biology Perspectives)
Abstract
Congenital Pouch Colon (CPC) is a rare anorectal anomaly common to northwestern India, specifically Rajasthan. Despite efforts to understand the clinical genetic makeup of CPC, no attempt on identifying non-coding RNAs was done. We have earlier reported CPC’s rare variants from whole exome sequencing (WES) across 18 affected samples in a total of 64 subjects. A Smith–Waterman algorithm was used to infer a couple of lncRNAs from WES samples of CPC with predictions from the Noncode database. Further screening and quantification using polymerase chain reaction (PCR), we ascertained interactions using Micro Scale Thermophoresis (MST). We report the role of lnc-EPB41-1-1 shown to be promiscuously interacting with KIF13A substantiating their role in regulation. View Full-TextKeywords:
long non coding RNA; whole exome sequencing; protein interaction; congenital pouch colon; microscale thermophoresis
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Gupta, S.; Gupta, N.; Tiwari, P.; Menon, S.; Mathur, P.; Kothari, S.L.; Nallapeta, S.; Medicherla, K.M.; Suravajhala, P. Lnc-EPB41-Protein Interactions Associated with Congenital Pouch Colon. Biomolecules 2018, 8, 95.
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