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Biomolecules 2018, 8(3), 78; https://doi.org/10.3390/biom8030078

Multi-Spectroscopic and Theoretical Analysis on the Interaction between Human Serum Albumin and a Capsaicin Derivative—RPF101

1
Institute of Chemistry, Universidade Federal Rural do Rio de Janeiro, BR-465 Km 7, 23970-000 Seropédica-RJ, Brazil
2
SENAI Innovation Institute for Green Chemistry, Rua Morais e Silva N° 53, Maracanã, 20271030 Rio de Janeiro-RJ, Brazil
3
Department of Pharmacy, University of São Paulo, Prof. Lineu Prestes Avenue, 580, Bl.13, 05508-900 Butanta, São Paulo-SP, Brazil
4
National Institute of Metrology, Quality and Technology, 25250-020 Duque de Caxias-RJ, Brazil
*
Author to whom correspondence should be addressed.
Received: 14 July 2018 / Revised: 13 August 2018 / Accepted: 16 August 2018 / Published: 23 August 2018
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Abstract

The interaction between the main carrier of endogenous and exogenous compounds in the human bloodstream (human serum albumin, HSA) and a potential anticancer compound (the capsaicin analogue RPF101) was investigated by spectroscopic techniques (circular dichroism, steady-state, time-resolved, and synchronous fluorescence), zeta potential, and computational method (molecular docking). Steady-state and time-resolved fluorescence experiments indicated an association in the ground state between HSA:RPF101. The interaction is moderate, spontaneous (ΔG° < 0), and entropically driven (ΔS° = 0.573 ± 0.069 kJ/molK). This association does not perturb significantly the potential surface of the protein, as well as the secondary structure of the albumin and the microenvironment around tyrosine and tryptophan residues. Competitive binding studies indicated Sudlow’s site I as the main protein pocket and molecular docking results suggested hydrogen bonding and hydrophobic interactions as the main binding forces. View Full-Text
Keywords: human serum albumin; RPF101; capsaicin; multi-spectroscopy; molecular docking human serum albumin; RPF101; capsaicin; multi-spectroscopy; molecular docking
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

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Chaves, O.A.; Tavares, M.T.; Cunha, M.R.; Parise-Filho, R.; Sant’Anna, C.M.R.; Netto-Ferreira, J.C. Multi-Spectroscopic and Theoretical Analysis on the Interaction between Human Serum Albumin and a Capsaicin Derivative—RPF101. Biomolecules 2018, 8, 78.

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